How to study aging with DNA methylation. 1,694 viewsJul 23, 2022 143 DISLIKE SHARE DOWNLOAD CLIP SAVE The Sheekey Science Show 28.1K subscribers What does DNA methylation inform us about the aging process? Does the presence of absence of methyl marks merely reflect the aging process or could they be causal? What is the best way to characterise the epigenetic marks - what about considering variance and pair-wise changes of specific CpG sites? There are many unaddressed questions in the fields of epigenetics and aging - so where are we at? In this video I spoke with Kirsten Seale about her research of the aging DNA methlyome, the approaches she takes and her thoughts on the field going forward. Find me on Twitter - https://twitter.com/EleanorSheekey Find Kirsten on Twitter - https://twitter.com/kirsten_blythe_ & check out her review article here; Seale, K., Horvath, S., Teschendorff, A. et al. Making sense of the ageing methylome. Nat Rev Genet (2022). https://doi.org/10.1038/s41576-022-00... Support the channel through PayPal - https://paypal.me/sheekeyscience?coun... through Patreon - https://www.patreon.com/TheSheekeySci... TIMESTAMPS Intro to epigenetics - 00:00 What is the DNA methylome - 01:15 DMPs vs. VMPs - 02:45 Primary vs. secondary aging - 04:30 Entropy - 06:30 PhD project - map of aging methylome - 08:00 Making sense of the methylome - 11:00 Future potential - 13:45 Advice for students - 14:45 Take homes & network analysis - 16:00 Please note that The Sheekey Science Show is distinct from Eleanor Sheekey's teaching and research roles at the University of Cambridge. The information provided in this show is not medical advice, nor should it be taken or applied as a replacement for medical advice. The Sheekey Science Show and guests assume no liability for the application of the information discussed. Icons in intro; "https://www.freepik.com/free-photos-v... "Background vector created by freepik - www.freepik.com Chapters 12 Comments rongmaw lin Add a comment... The Sheekey Science Show Pinned by The Sheekey Science Show The Sheekey Science Show 2 days ago If you enjoyed our conversation, check out a link to Kirsten's review in the description! 4 In Hoc Signo Vinces In Hoc Signo Vinces 1 day ago Even though DNA methylation based 'aging clock' approaches are in vogue right now, I'd love to see someone produce a method for calculating aging based on the levels of advanced glycation end products in the body, because I believe that approach is one of the keys to slowing down / reversing aging. Even exogenous AGEs were able to negate the longevity benefits of calorie restricted diet (the most studied longevity approach out there) in animal models. 4 Ken Otwell Ken Otwell 1 day ago Fascinating and clear-eyed interview - good job! The Sheekey Science Show Peter Peter 1 day ago (edited) Thanks for this discussion. Mind wandering: I wonder is the protein changes observed (more youthful) in the 2020 Conboy plasma exhange experiments were driven by changes in the epigenome? If so, how this might occur? Conboys saw changes after one month but did not test for longer durations. The Sheekey Science Show Martin Tippins Martin Tippins 22 hours ago I would think that the epigenetic methyl markers would ALTER the proteins produced by the genes. And humans could easily adjust these markers. If its not that simple, why not -lol? Excuse my layman logic (LL). What The Function What The Function 6 hours ago That was a lot of data. Can you do a takeaways video Pat McCormick Pat McCormick 1 day ago Look at what The Salk Institute found when researching Acetyl-Coenzyme A!!! What The Function What The Function 6 hours ago Plot twist: both of these ladies are in their late 60's 1 Kingsize Kingsize 1 day ago 🔥🔥🔥 Alex Berukoff Alex Berukoff 1 day ago awesome The Sheekey Science Show Michael Vaughan Michael Vaughan 1 day ago you will straight up look like that author in 60 years i swear hahah,