Glycoprote

Jump to navigationJump to searchNot to be confused with peptidoglycan, proteoglycan, or glycopeptide.

N-linked protein glycosylation (N-glycosylation of N-glycans) at Asn residues (Asn-x-Ser/Thr motifs) in glycoproteins.[1]
Glycoproteins are proteins which contain oligosaccharide chains (glycans) covalently attached to amino acid side-chains. The carbohydrate is attached to the protein in a cotranslational or posttranslational modification. This process is known as glycosylation. Secreted extracellular proteins are often glycosylated.
In proteins that have segments extending extracellularly, the extracellular segments are also often glycosylated. Glycoproteins are also often important integral membrane proteins, where they play a role in cell–cell interactions. It is important[according to whom?] to distinguish endoplasmic reticulum-based glycosylation of the secretory system from reversible cytosolic-nuclear glycosylation. Glycoproteins of the cytosol and nucleus can be modified through the reversible addition of a single GlcNAc residue that is considered reciprocal to phosphorylation and the functions of these are likely to be additional regulatory mechanism that controls phosphorylation-based signalling.[2] In contrast, classical secretory glycosylation can be structurally essential. For example, inhibition of asparagine-linked, i.e. N-linked, glycosylation can prevent proper glycoprotein folding and full inhibition can be toxic to an individual cell. In contrast, perturbation of glycan processing (enzymatic removal/addition of carbohydrate residues to the glycan), which occurs in both the endoplasmic reticulum and Golgi apparatus, is dispensable for isolated cells (as evidence by survival with glycosides inhibitors) but can lead to human disease (congenital disorders of glycosylation) and can be lethal in animal models. It is therefore likely that the fine processing of glycans is important for endogenous functionality, such as cell trafficking, but that this is likely to have been secondary to its role in host-pathogen interactions. A famous example of this latter effect is the ABO blood group system.[citation needed]

Types of glycosylation[edit]

There are several types of glycosylation, although the first two are the most common.

Monosaccharides[edit]


Eight sugars commonly found in glycoproteins.
Monosaccharides commonly found in eukaryotic glycoproteins include:[4]:526
The principal sugars found in human glycoproteins[5]
SugarTypeAbbreviation
β-D-GlucoseHexoseGlc
β-D-GalactoseHexoseGal
β-D-MannoseHexoseMan
α-L-FucoseDeoxyhexoseFuc
N-AcetylgalactosamineAminohexoseGalNAc
N-AcetylglucosamineAminohexoseGlcNAc
N-Acetylneuraminic acidAminononulosonic acid
(Sialic acid)		NeuNAc
XylosePentoseXyl
The sugar group(s) can assist in protein folding, improve proteins' stability and are involved in cell signalling.

Examples[edit]

One example of glycoproteins found in the body is mucins, which are secreted in the mucus of the respiratory and digestive tracts. The sugars when attached to mucins give them considerable water-holding capacity and also make them resistant to proteolysis by digestive enzymes.
Glycoproteins are important for white blood cell recognition.[citation needed] Examples of glycoproteins in the immune system are:
  • molecules such as antibodies (immunoglobulins), which interact directly with antigens.
  • molecules of the major histocompatibility complex (or MHC), which are expressed on the surface of cells and interact with T cells as part of the adaptive immune response.
  • sialyl Lewis X antigen on the surface of leukocytes.
H antigen of the ABO blood compatibility antigens. Other examples of glycoproteins include:
  • gonadotropins (luteinizing hormone a follicle-stimulating hormone)
  • glycoprotein IIb/IIIa, an integrin found on platelets that is required for normal platelet aggregation and adherence to the endothelium.
  • components of the zona pellucida, which surrounds the oocyte, and is important for sperm-egg interaction.
  • structural glycoproteins, which occur in connective tissue. These help bind together the fibers, cells, and ground substance of connective tissue. They may also help components of the tissue bind to inorganic substances, such as calcium in bone.
  • Glycoprotein-41 (gp41) and glycoprotein-120 (gp120) are HIV viral coat proteins.
Soluble glycoproteins often show a high viscosity, for example, in egg white and blood plasma.
Variable surface glycoproteins allow the sleeping sickness Trypanosoma parasite to escape the immune response of the host.
The viral spike of the human immunodeficiency virus is heavily glycosylated.[7] Approximately half the mass of the spike is glycosylation and the glycans act to limit antibody recognition as the glycans are assembled by the host cell and so are largely 'self'. Over time, some patients can evolve antibodies to recognise the HIV glycans and almost all so-called 'broadly neutralising antibodies (bnAbs) recognise some glycans. This is possible mainly because the unusually high density of glycans hinders normal glycan maturation and they are therefore trapped in the premature, high-mannose, state.[8][9] This provides a window for immune recognition. In addition, as these glycans are much less variable than the underlying protein, they have emerged as promising targets for vaccine design.[10]

Hormones[edit]

Hormones that are glycoproteins include:

Functions[edit]

Some functions served by glycoproteins[4]:524
FunctionGlycoproteins
Structural moleculeCollagens
Lubricant and protective agentMucins
Transport moleculeTransferrin, ceruloplasmin
Immunologic moleculeImmunoglobulins,[11] histocompatibility antigens
HormoneHuman chorionic gonadotropin (HCG), thyroid-stimulating hormone (TSH)
EnzymeVarious, e.g., alkaline phosphatase, patatin
Cell attachment-recognition siteVarious proteins involved in cell–cell (e.g., sperm–oocyte), virus–cell, bacterium–cell, and hormone–cell interactions
Antifreeze proteinCertain plasma proteins of coldwater fish
Interact with specific carbohydratesLectins, selectins (cell adhesion lectins), antibodies
ReceptorVarious proteins involved in hormone and drug action
Affect folding of certain proteinsCalnexin, calreticulin
Regulation of developmentNotch and its analogs, key proteins in development
Hemostasis (and thrombosis)Specific glycoproteins on the surface membranes of platelets

Analysis[edit]

A variety of methods used in detection, purification, and structural analysis of glycoproteins are[4]:525[11][12]
Some important methods used to study glycoproteins
MethodUse
Periodic acid-Schiff stainDetects glycoproteins as pink bands after electrophoretic separation.
Incubation of cultured cells with glycoproteins as radioactive decay bandsLeads to detection of a radioactive sugar after electrophoretic separation.
Treatment with appropriate endo- or exoglycosidase or phospholipasesResultant shifts in electrophoretic migration help distinguish among proteins with N-glycan, O-glycan, or GPI linkages and also between high mannose and complex N-glycans.
Agarose-lectin column chromatography, lectin affinity chromatographyTo purify glycoproteins or glycopeptides that bind the particular lectin used.
Lectin affinity electrophoresisResultant shifts in electrophoretic migration help distinguish and characterize glycoforms, i.e. variants of a glycoprotein differing in carbohydrate.
Compositional analysis following acid hydrolysisIdentifies sugars that the glycoprotein contains and their stoichiometry.
Mass spectrometryProvides information on molecular mass, composition, sequence, and sometimes branching of a glycan chain. It can also be used for site-specific glycosylation profiling.[11]
NMR spectroscopyTo identify specific sugars, their sequence, linkages, and the anomeric nature of glycosidic chain.
Multi-angle light scatteringIn conjunction with size-exclusion chromatography, UV/Vis absorption and differential refractometry, provides information on molecular mass, protein-carbohydrate ratio, aggregation state, size, and sometimes branching of a glycan chain. In conjunction with composition-gradient analysis, analyzes self- and hetero-association to determine binding affinity and stoichiometry with proteins or carbohydrates in solution without labeling.
Dual Polarisation InterferometryMeasures the mechanisms underlying the biomolecular interactions, including reaction rates, affinities and associated conformational changes.
Methylation (linkage) analysisTo determine linkage between sugars.
Amino acid or cDNA sequencingDetermination of amino acid sequence.

See also[edit]

Notes and references[edit]

  1. ^ Ruddock, L. W.; Molinari, M. (2006). "N-glycan processing in ER quality control". Journal of Cell Science. 119 (21): 4373–4380. doi:10.1242/jcs.03225. PMID 17074831.
  2. ^ Funakoshi Y, Suzuki T (January 2009). "Glycobiology in the cytosol: The bitter side of a sweet world". Biochim. Biophys. Acta. 1790 (2): 81–94. doi:10.1016/j.bbagen.2008.09.009. PMID 18952151.
  3. ^ Stepper, Judith; Shastri, Shilpa; Loo, Trevor S.; Preston, Joanne C.; Novak, Petr; Man, Petr; Moore, Christopher H.; Havlíček, Vladimír; Patchett, Mark L. (18 January 2011). "CysteineS-glycosylation, a new post-translational modification found in glycopeptide bacteriocins". FEBS Letters. 585 (4): 645–650. doi:10.1016/j.febslet.2011.01.023. ISSN 0014-5793. PMID 21251913.
  4. ^ Jump up to:a b c Robert K. Murray, Daryl K. Granner & Victor W. Rodwell: "Harper's Illustrated Biochemistry 27th Ed.", McGraw–Hill, 2006
  5. ^ Glycan classification SIGMA
  6. ^ Theerasilp S, Kurihara Y (August 1988). "Complete purification and characterization of the taste-modifying protein, miraculin, from miracle fruit". J. Biol. Chem. 263 (23): 11536–9. PMID 3403544.
  7. ^ Pritchard, Laura K.; Vasiljevic, Snezana; Ozorowski, Gabriel; Seabright, Gemma E.; Cupo, Albert; Ringe, Rajesh; Kim, Helen J.; Sanders, Rogier W.; Doores, Katie J. (16 June 2015). "Structural Constraints Determine the Glycosylation of HIV-1 Envelope Trimers". Cell Reports. 11 (10): 1604–1613. doi:10.1016/j.celrep.2015.05.017. ISSN 2211-1247. PMC 4555872. PMID 26051934.
  8. ^ Pritchard, Laura K.; Spencer, Daniel I. R.; Royle, Louise; Bonomelli, Camille; Seabright, Gemma E.; Behrens, Anna-Janina; Kulp, Daniel W.; Menis, Sergey; Krumm, Stefanie A. (24 June 2015). "Glycan clustering stabilizes the mannose patch of HIV-1 and preserves vulnerability to broadly neutralizing antibodies". Nature Communications. 6: 7479. Bibcode:2015NatCo...6.7479P. doi:10.1038/ncomms8479. PMC 4500839. PMID 26105115.
  9. ^ Behrens, Anna-Janina; Vasiljevic, Snezana; Pritchard, Laura K.; Harvey, David J.; Andev, Rajinder S.; Krumm, Stefanie A.; Struwe, Weston B.; Cupo, Albert; Kumar, Abhinav (10 March 2016). "Composition and Antigenic Effects of Individual Glycan Sites of a Trimeric HIV-1 Envelope Glycoprotein". Cell Reports. 14 (11): 2695–2706. doi:10.1016/j.celrep.2016.02.058. ISSN 2211-1247. PMC 4805854. PMID 26972002.
  10. ^ Crispin, Max; Doores, Katie J (1 April 2015). "Targeting host-derived glycans on enveloped viruses for antibody-based vaccine design". Current Opinion in Virology. Viral pathogenesis • Preventive and therapeutic vaccines. 11: 63–69. doi:10.1016/j.coviro.2015.02.002. PMC 4827424. PMID 25747313.
  11. ^ Jump up to:a b c Maverakis E, Kim K, Shimoda M, Gershwin M, Patel F, Wilken R, Raychaudhuri S, Ruhaak LR, Lebrilla CB (2015). "Glycans in the immune system and The Altered Glycan Theory of Autoimmunity". J Autoimmun. 57 (6): 1–13. doi:10.1016/j.jaut.2014.12.002. PMC 4340844. PMID 25578468.
  12. ^ Dell A (2001). "Glycoprotein Structure Determination by Mass Spectrometry". Science. 291 (5512): 2351–2356. Bibcode:2001Sci...291.2351D. doi:10.1126/science.1058890. ISSN 0036-8075. PMID 11269315.

External links[edit]