human gut bacteria in the occurrence of neuropsychiatric disorders

Interplay Between Human Gut Bacteria Escherichia coli and Lactobacillus mucosae in the Occurrence of Neuropsychiatric Disorders in Mice Jeon-Kyung Kim 1 2, Kyung-Eon Lee 1 2, Sang-Ah Lee 1 2, Hyo-Min Jang 1 2, Dong-Hyun Kim 1 2 Affiliations expand PMID: 32158447 PMCID: PMC7051986 DOI: 10.3389/fimmu.2020.00273 Free PMC article Abstract To understand the roles of human gut bacteria in the occurrence of neuropsychiatric disorders, we isolated inflammatory Escherichia coli K1 and anti-inflammatory Lactobacillus mucosae from healthy human feces and examined their effects on the occurrence of altered microbiota, cognitive decline, and depression in mice. Oral gavage of Escherichia coli K1 caused colitis, cognitive decline, and depression in mice in the elevated plus maze, tail suspension, and forced swimming tasks. However, NK41 treatment reduced K1-induced cognitive decline and anxiety/depression. Furthermore, NK41 treatment increased K1-suppressed brain-derived neurotrophic factor (BDNF) expression and BDNF+/NeuN+ cell population and suppressed K1-induced NF-κB activation and LPS+/Iba1+ and NF-κB+/Iba1+ (microglial) cell populations in the hippocampus. NK41 treatment also suppressed K1-induced TNF-α and LPS levels in the blood and TNF-α expression, myeloperoxidase activity, NF-κB+/CD11c+ and CD11b+/CD11c+ cell populations in the colon. Furthermore, NK41 treatment decreased K1-induced colonic MUC2 expression, gut Proteobacteria population, and fecal LPS levels and modified the bacterial abundance related to polysaccharide breaking and biosynthesis. In conclusion, the overgrowth of inflammatory bacteria such as Escherichia coli in the gastrointestinal tract can cause neuropsychiatric disorders with gut microbiota alteration and the superiority of anti-inflammatory bacteria such as Lactobacillus mucosae can alleviate neuropsychiatric disorders with the attenuation of altered microbiota. Keywords: brain; colon; gut bacteria; inflammation; neuropsychiatric disorder. Copyright © 2020 Kim, Lee, Lee, Jang and Kim. Figures Figure 1 Figure 1 Effects of gut bacteria K1… Figure 2 Figure 2 Effects of K1 and NK41… Figure 3 Figure 3 NK41 suppressed K1-induced altered microbiota… Figure 4 Figure 4 NK41 signiticantly suppressed K1-induced gut… Figure 5 Figure 5 NK41 signiticantly suppressed K1-induced neuropsychiatric… Figure 6 Figure 6 Interplay between Escherichia coli K1… Similar articles A Probiotic Lactobacillus gasseri Alleviates Escherichia coli-Induced Cognitive Impairment and Depression in Mice by Regulating IL-1β Expression and Gut Microbiota. Yun SW, Kim JK, Lee KE, Oh YJ, Choi HJ, Han MJ, Kim DH. Nutrients. 2020 Nov 10;12(11):3441. doi: 10.3390/nu12113441. PMID: 33182607 Free PMC article. Bifidobacteria-Fermented Red Ginseng and Its Constituents Ginsenoside Rd and Protopanaxatriol Alleviate Anxiety/Depression in Mice by the Amelioration of Gut Dysbiosis. Han SK, Joo MK, Kim JK, Jeung W, Kang H, Kim DH. Nutrients. 2020 Mar 26;12(4):901. doi: 10.3390/nu12040901. PMID: 32224881 Free PMC article. Lactobacillus mucosae and Bifidobacterium longum Synergistically Alleviate Immobilization Stress-Induced Anxiety/Depression in Mice by Suppressing Gut Dysbiosis. Han SK, Kim DH. J Microbiol Biotechnol. 2019 Sep 28;29(9):1369-1374. doi: 10.4014/jmb.1907.07044. PMID: 31564078 High-fat diet causes psychiatric disorders in mice by increasing Proteobacteria population. Jeong MY, Jang HM, Kim DH. Neurosci Lett. 2019 Apr 17;698:51-57. doi: 10.1016/j.neulet.2019.01.006. Epub 2019 Jan 4. PMID: 30615977 Immobilization stress-induced Escherichia coli causes anxiety by inducing NF-κB activation through gut microbiota disturbance. Jang HM, Lee KE, Lee HJ, Kim DH. Sci Rep. 2018 Sep 17;8(1):13897. doi: 10.1038/s41598-018-31764-0. PMID: 30224732 Free PMC article. See all similar articles Cited by 3 articles Orally Administered Antibiotics Vancomycin and Ampicillin Cause Cognitive Impairment With Gut Dysbiosis in Mice With Transient Global Forebrain Ischemia. Lee KE, Kim JK, Kim DH. Front Microbiol. 2020 Nov 26;11:564271. doi: 10.3389/fmicb.2020.564271. eCollection 2020. PMID: 33324357 Free PMC article. A Probiotic Lactobacillus gasseri Alleviates Escherichia coli-Induced Cognitive Impairment and Depression in Mice by Regulating IL-1β Expression and Gut Microbiota. Yun SW, Kim JK, Lee KE, Oh YJ, Choi HJ, Han MJ, Kim DH. Nutrients. 2020 Nov 10;12(11):3441. doi: 10.3390/nu12113441. PMID: 33182607 Free PMC article. The extracellular vesicle of gut microbial Paenalcaligenes hominis is a risk factor for vagus nerve-mediated cognitive impairment. Lee KE, Kim JK, Han SK, Lee DY, Lee HJ, Yim SV, Kim DH. Microbiome. 2020 Jul 15;8(1):107. doi: 10.1186/s40168-020-00881-2. PMID: 32669127 Free PMC article. References Xu W, Anderson ME, Kotarba AE, Davis J, Robinson JK, Van Nostrand WE. Cerebral microvascular rather than parenchymal amyloid-β protein pathology promotes early cognitive impairment in transgenic mice. J Alzheimers Dis. (2014) 38:621–32. 10.3233/JAD-130758 - DOI - PMC - PubMed Dinan TG, Cryan JF. Brain-gut-microbiota axis and mental health. Psychosom Med. (2017) 79:920–6. 10.1097/PSY.0000000000000519 - DOI - PubMed Neufeld KM, Kang N, Bienenstock J, Foster JA. Reduced anxiety-like behavior and central neurochemical change in germ-free mice. Neurogastroenterol Motil. (2011) 23:255–64. 10.1111/j.1365-2982.2010.01620.x - DOI - PubMed Silverman MN, Sternberg EM. Glucocorticoid regulation of inflammation and its functional correlates: from HPA axis to glucocorticoid receptor dysfunction. Ann N Y Acad Sci. (2012) 1261:55–63. 10.1111/j.1749-6632.2012.06633.x - DOI - PMC - PubMed Smith SM, Vale WW. The role of the hypothalamic-pituitary-adrenal axis in neuroendocrine responses to stress. Dialogues Clin Neurosci. (2006) 8:383–95. - PMC - PubMed Show all 51 references Publication types Research Support, Non-U.S. Gov't Related information MedGen LinkOut - more resources Full Text Sources Europe PubMed Central Frontiers Media SA PubMed Central Miscellaneous NCI CPTAC Assay Portal