NAD+ in Aging: Role of Nicotinamide Riboside and Nicotinamide Mononucleotide

NAD+ in Aging: Role of Nicotinamide Riboside and Nicotinamide Mononucleotide 506,433 viewsPremiered Jan 28, 2020 FoundMyFitness 439K subscribers In this episode, Rhonda explains what NAD+, why it is so important for aging and why it declines with age. She discusses some of the popular NAD+ boosters like nicotinamide riboside and nicotinamide mononucleotide, what animal and human data says and some concluding thoughts. NAD+ is crucial for our survival. Small amounts of it can be obtained in the diet, but the body recycles it to get most of what it needs. Unfortunately, day to day living plus normal aging can cause NAD+ levels to drop. This causes metabolic and mitochondrial dysfunction, as well as many of the other problems and conditions associated with aging like elevated DNA damage. NAD+ levels can go up when we exercise or fast, but NAD+ precursors like nicotinamide riboside and nicotinamide mononucleotide can also increase NAD+ levels in a dose-dependent manner. However, most of the data on NAD+ boosters comes from animal studies. Whether these boosters are effective – and safe – long-term in humans is still a big question. ▶︎ Get the episode's show notes, timeline, and more https://www.foundmyfitness.com/episod... Chapters 00:00:00 - Introduction 00:01:01 - Aging 00:02:47 - Overview 00:07:35 - Depletion 00:09:19 - Supplementation 00:11:26 - Animal studies 00:13:47 - Cancer 00:15:57 - Dosage 00:25:44 - Human data 00:28:25 - Storage 00:29:00 - Recap ▶︎ Detailed overview of NAD+ https://www.foundmyfitness.com/topics... ▶︎ Detailed overview of nicotinamide riboside https://www.foundmyfitness.com/topics... ▶︎ Detailed overview of nicotinamide mononucleotide https://www.foundmyfitness.com/topics... ▶︎ Did you enjoy this podcast? If you want to support me in getting more of this free information out to the public, please consider becoming an FMF premium member! In doing so, you'll also get access to exclusive member benefits, such as a private podcast feed offering early access and occasional bonus episodes, monthly live Q&As with me, a members-only library that includes presentation slides with all of the supporting figures, graphics, references, and quotes. Also included are Rhonda's private notes on topics discussed in the podcast, bi-monthly member-exclusive Science News Digest email, and more. Thank you for your consideration! Learn more here: https://www.foundmyfitness.com/premium ▶︎ Gain valuable insights by running your raw genetic data through the FoundMyFitness report https://www.foundmyfitness.com/geneti... Chapters 1,276 Comments rongmaw lin Add a comment... FoundMyFitness Pinned by FoundMyFitness FoundMyFitness 2 years ago (edited) 00:00:00 - Intro. 00:00:48 - Nicotinamide riboside supplementation in humans has been shown to raise NAD+ levels in white blood cells. 00:02:47 - Overview of NAD+ 00:05:12 - NAD+ plays an important role in mitigating DNA damage. 00:05:30 - Excessive DNA damage can decrease NAD+ levels by 20-30%. 00:05:42 - Lymphoblastoid cells from centenarians show increased PARP-1 activity. 00:05:55 - Between different species of mammals, higher PARP-1 activity is strongly associated with a longer lifespan. 00:06:36 - Lower organism evidence: A second copy of SIR2, a yeast sirtuin, gave rise to a 30% extension of lifespan. 00:06:43 - NAD+ is required for the activation of sirtuins, which epigenetically regulate a variety of stress response-related processes. 00:07:03 - Energy stress, such as from fasting, exercise, or caloric restriction, increase the NAD+:NADH ratio. 00:07:37 - NAD+ becomes depleted in the brain, heart, and skeletal muscle as we age. 00:08:13 - Decline in NAD+ levels have been associated with many broad categories of clinical disease such as neurodegenerative, cardiovascular, infectious, musculoskeletal. 00:08:48 - NAD+ supplementation has been shown to increase lifespan in models of aging in yeast, worms, and rodents. 00:09:48 - In contrast to de novo synthesis, the predominant source of NAD+ in the body is via the salvage pathway, which is subject to feedback inhibition by nicotinamide. 00:11:56 - Animal studies have shown nicotinamide riboside supplementation is able to increase insulin sensitivity, increase endurance and strength, reverse mitochondrial damage, increase mitochondrial biogenesis, and reverse muscular atrophy. 00:12:30 - Nicotinamide riboside has shown positive effects on the brain in animal, such as increased neurogenesis, decreased cognitive deterioration, decreased amyloid-beta production, and increased synaptic plasticity. 00:12:50 - Nicotinamide mononucleotide has been shown in animals to counter an obesogenic diet, improve markers of metabolic health, improve heart function, and improve cognition and memory. 00:13:10 - A 12-month dietary study of nicotinamide mononucleotide suppressed age-associated body weight gain, enhanced energy metabolism, promoted physical activity, improved insulin sensitivity and plasma lipid profile, and ameliorated eye function and other pathophysiologies in mice. 00:13:47 - NMN has been shown to increase cancer progression in a specialized senescent cell-dependent pancreatic cancer model in mice. 00:15:57 - Plausibility of dosing: comparing animal and human doses. 00:18:05 - Tissue-specific metabolism and bioavailability of NR and NMN. 00:24:32 - Oral administration of NAD produces NR and NMN in the intestine. 00:24:19 - Intravenous administration of NAD, rather than its precursors, shows interesting results: an increase of NAD content and sirtuin expression in the hypothalamic region of the brain. 00:26:15 - Human study #1: 250mg of NR + 50 mg pterostilbene increased blood NAD+ levels by 40%, while a double dose (500 mg NR + 100 mg pterostilbene) increased blood NAD+ levels by 90%. Additionally, there was a decrease in blood pressure, and ALT (a marker for liver health). 00:27:12 - Human study #2: 500mg NR twice daily for six weeks increased NAD+ levels in white blood cells by 60%. 00:29:08 - Concluding thoughts and recap. 261 Chuck A Chuck A 2 years ago Congratulations Rhonda you have smashed the record for total mentions of "Nicotinamide and Nicotinamide Mononucleotide" without stuttering, giggling or entering a comatose state. Kudos! 191 DAVID EVANS DAVID EVANS 2 years ago (edited) Rarely do we find in the study of nutrition, detail like presented in this video, yet at the same time Rhonda is able to get to her final conclusion without leaving everyone in the weeds. Extremely well done. Combined with Dr. Nicholas Pericone and Dr. Jason Fung, we are starting to get a handle on what is necessary to make critical changes in our life for optimal health... 10 pablopolski pablopolski 2 years ago Concise, comprehensive, and articulate. I don't even need to speed it up to get through the information. Well done. 377 FoundMyFitness Chris Benton Chris Benton 2 years ago Excellent lecture, well put together, a good balance of pros and cons, useful diagrams and a clear summary at the end. Perfect presentation technique. 112 FoundMyFitness Scott Joshua Scott Joshua 2 years ago What a herculean effort and a testament to your ability to grind it out for hours on end. You’re incredible Rhonda. 16 FoundMyFitness Rich Rozmarn Rich Rozmarn 2 years ago Great presentation, thank you so much. I take NAD with R-Lipoic acid, D-Ribose and Resveratrol for a pre workout. I work out 3-4 times a week and feel as good as when i was 40, now 60. 53 Tony Westbrook Tony Westbrook 2 years ago I learn so much here by your presentation and documentation. Thank you Rhonda for taking, what must be a lot of time to put all of this documentation together! 28 FoundMyFitness Dr. Nancy McClure-Galli Dr. Nancy McClure-Galli 2 years ago So great to have the micro cellular pathways broken down this way. Also, the analysis of both animal and human studies. Thank you so much for the effortless way you explain potentially mind boggling processes. Helps so much in the decision making process. No Limit No Limit 2 years ago A very professional very well presented lecture . English is my 4th. language and I did not have any real problem to understand it and learn from it. Doc, I really appreciate your effort to create this video. Thank you. 8 FoundMyFitness John Curtis John Curtis 2 years ago Looks like more studies on humans are needed since the animal studies seem so promising. I've been taking nicotinamide riboside (250mg) and pterostbene (50mg) for over 5 years now. It's the only supplement I take. I'm 66 years of age, 5'10" tall and weigh ~155lbs. I feel in perfect health despite a few creaks and hiccups in my giddy-up (heh). I'd also like to think my diet, while moderate, is well-rounded in a healthy sense. Lots of fruits, vegetables and a modicum of meat. I'm not much into junk foods and the like. I sleep well at night, averaging ~8hrs, and function well all day long. My energy levels remain constant pretty much all day, though I'm not going to claim I have the same level of endurance I had at 18-20 years of age. I bike and walk for exercise and pleasure. I suffer none of the maladies I see beginning to manifest in friends and colleagues in my age cohort. And I still have my marbles. Or.....at least I think I do. Friends may disagree. ;-) Now is this due to the supplement? Possibly. Physically I feel like my body is functioning at a nice "steady state" level though the vicissitudes of time do appear when I look in the mirror. There's nothing to be done, overall, about the ever constant, albeit slow, aging that it shows. Gray hair, wrinkles and the like. That's just life. That said I feel like I'm motoring along nicely. But in the interest of full disclosure I'm unclear if this is entirely due to the supplement I'm taking. A portion of this might be due to it; but I've a 90 year old father who still runs +20 miles a week, every week. As he has done since I was a toddler. And my mother is still alive and kicking, though a bit more frail. Both still have their minds, too. So the supplement might be noteworthy, but I suspect so, too, is my genetic coding. So that needs to be considered when evaluating the merits of my health. In any case I see nothing wrong with supplementing the diet with it. I take no vitamins and eat a moderate, rounded diet. It would be nice to see the results of more human-oriented studies of nicotinamide and the like. I think there's merit to it keeping one healthy for as long as one lives. And regardless of how long you live staying healthy is the whole point, isn't it? John~ American Net'Zen profitfever profitfever 2 years ago So glad you covered this. Been listening to lifespan on audio book and he's one of the leading scientists on this research. 8 Rob Lovegreen Rob Lovegreen 2 years ago Because of you, I started loving myself with broccoli sprouts. ❤️ I grow them too. Sulforaphane for the win! Thank you 🥦 60 SpockBoy SpockBoy 2 years ago (edited) Thank you so much for bringing us this information Doctor. Using your great intelligence for altruistic purposes is both rare and wonderful. 3 Ramon Mensink Ramon Mensink 1 year ago Great video Rhonda! I was wondering if it makes sense to take a NR/NMN supplement while also ingesting carbs/protein/fat. As you also explain in the video, the NAD/NADH ratio in the cell increases when fasting/CR/exercising. Then such a NAD boosting supplement would need to be taken in a fasted state wouldn't it? Or is the pathway of either NR/NMN supplement somehow separated from the metabolic pathway when a cell is in a fasted state? 2 David David 2 years ago Great video Rhonda, thank you for all you're doing and the quality information with such strong scientific detail. 2 Hank Nelson Hank Nelson 2 years ago The wiggle room for fat lose I find is from a developed weight training program that has been done over 1 year. The other is the reduction of carbohydrates and sugar. Even if I take an excess of calories my body's stubborn predisposition actually burns more calories to maintain homeostasis. JosetteBadger JosetteBadger 2 weeks ago You remind me of my favorite med school professor- one of my favorite instructors of all time Thanks for the clear and concise video! KenG KenG 8 months ago Excellent analysis and much appreciated! I love your presentation speed and style! 1 Cat Man Cat Man 1 year ago I've been taking a low doseage of NMN for a couple of weeks now. I started with 100mg in the morning and 50mg at night on the first day, then 50mg the next morning. I hadn't been expecting any results for at least a week or so. About 2 hours after taking that second morning dose I went out shopping and was astonished to find my legs wanting to walk faster and faster, as if under their own volition. I've not walked like that in almost 7 years. I've had a number of strokes and suffer numerous symptoms, manly to do with inhibitted activity levels. My mind is foggy most of the time and the NMN has not done anything for that, but I certainly feel the difference in energy levels, it's really amazing. I also found that after a few days I had to stop the NMN for a couple of days as it seemed to be making me feel more groggy, so I now take it 2 days on, 2 days off. The brand I'm using is an American Brand called RevGenetics, and it's called Advanced NMN, in capsule form. I know it's also available in powder form too. It's quite expensive even in capsule form and would cost the earth to take it regularly at the doseages recommended in this video, but I can assure anyone that the effects at the doseage I am on are truly amazing. 5 Karla Jean Karla Jean 2 years ago 5 months later and I'm still coming back to relisten to this video. Very informative and so well put together. 2 Noveleffect Noveleffect 2 years ago I really appreciate the chemical reaction explained to me along with the reaction diagrams. So rare to find anything this thorough 4 TRANSFORMATION CENTRAL TRANSFORMATION CENTRAL 2 months ago Hi Rhonda, Have you considered an update on your analysis of NAD boosters? Enjoyed your take on them and certainly interested in your opinion! 2 Morrocco Method Int'l Morrocco Method Int'l 2 years ago So helpful + informative for learning about aging. Thank you for this video! 2 quigonreturns again quigonreturns again 6 months ago Hello! Would love to know your thoughts on methyl group depletion as it relates to Niacinamide supplementation. I have been taking it at 500mg/day, with no ill effects. However, I am cautious about the long term affects of supplementation. Thanks! And love your content! Dustin Phillips Dustin Phillips 2 years ago Thank you for the video. I have been taking Tru Nigen (NR) for about two months at 350 mgs. What I have noticed is a slight increase in energy, and focus. I feel like I have also lost some body fat. However, I stay in Ketosis and use intermittent fasting 4 days a week. However, I plan to stop taking it once my bottle runs out because of the information presented in this video. I don't see the benefits outweighing the potential downsides. Thanks again. 11 Anita Venkataramana Anita Venkataramana 1 month ago How Lucky we are to have Dr. Patrick in our lives. She is amazing. Knowledge just oozes out of her.. June Allison June Allison 1 year ago Dr. Rhonda this is the best explanation I've heard on this topic. So thorough and well thought out. Thank you❤ 2 Ivan Valdez, PhD Ivan Valdez, PhD 1 year ago Fantastic job, Dr. Patrick. Do you think that sublingual NR/NMN would be effective at increasing NAD+ levels in different tissues And not just blood, similar to Intravenous administration, by bypassing the liver? Andrew Moore Andrew Moore 2 years ago Superb. So much information explained so efficiently in 30mins. I feel very well informed now, thank you! 1 Karen Andrea Brown Karen Andrea Brown 1 year ago I started taking NMN and trans-resveratrol because of David Sinclair on Rhonda Patrick's podcast! All my health markers have gone up since! I didn't notice those effects when I was taking elysium's NR. Wolfram Luchner Wolfram Luchner 2 years ago Exemplary summary. I love how dense with information your videos are. Quite a rarity on youtube! Keep up the good work and thank you for your high quality content! 7 FoundMyFitness Ja Wi Ja Wi 2 years ago Good work! You certainly did the research and can explain it with great accuracy. Thank you. Vedran Simic Vedran Simic 2 years ago Very interesting. From your video's from Satchin Panda I learned the benefits of time restricted eating regaring insulin sensitivity, sleep, cardiovascular aspect etcetera. Do you have any information whether NAD+ increases also with time restricted feeding and which window of eating you should use? Or is there only information about fasting and increasing NAD+ and if that is the case how long you should fast for increasing NAD+. 1 uncle Joe uncle Joe 2 years ago Exciting developments. Great to know scientists are working so hard on our behalf in this crazy world. In the meantime I'll continue my exercise, sauna, and clean diet. Thanks! 1 Joseph Khadige Joseph Khadige 1 year ago Great presentation Doc. Thank you. Please excuse my ignorance in this matter but to make sure I am understanding it accurately, if a cancer patient is regularly fasting and exercising (which leads to increase of NAD levels) would that increase the spread and developing of the cancer cells? Kuo-Ying Wang Kuo-Ying Wang 8 months ago Excellent and brilliant work. Thank you very much indeed. The literature reviewers are amazingly detailed and strikes the key points of the findings from the researches. Harvey Holloway Harvey Holloway 2 years ago Great video. I am 70 and a health fanatic, and still in perfect health. I have a massive coordinated program that did not include this. Now I will be going out today and buying Niacin. I am going to start immediately. Have a wonderful day. ☺😎🐰 4 gururaj bsavakar gururaj bsavakar 2 years ago Congratulations! Firstly for the , pleasant, patient presentation, with amazing revelations of test results of NAD, decline with age, using boosters of NAD, & Alternate pathways which work usually but with exceptions!!! Extremely A Great MASTERLY STUDY/ & presentation of all pros- cons of all eventualities . With a final recap!! Thanks for this useful educative share! Many regards! 5 FoundMyFitness Tom Provan Tom Provan 2 years ago This is definitely the best most informative and comprehensive piece of information so far delivered on this topic as far as I am concerned thank you for this video B D B D 11 months ago (edited) Excellent!! Conclusion: Fasting, less calories, regular exercise increases NAD level👍 We all need to wait for using supplement at this point till more data available! 1 Roger Bowman's Fitness & Wellness Coaching Roger Bowman's Fitness & Wellness Coaching 5 months ago Stellar presentation as always, thank you Dr. Patrick! Bakhtiyar Neyman Bakhtiyar Neyman 2 years ago Instantly one of my favorites videos on Youtube. Thank you so much, Rhonda! 2 Cuenta de Youtube Cuenta de Youtube 2 years ago Best unbiased recap of NAD+, NR and NMN. Thank you! Suscribed! 3 alfred Vincent alfred Vincent 2 years ago Great concise presentation as always. Thank you. 2 Cris Clourt Cris Clourt 2 years ago Thanks Rhonda for your amazing research! Do you know what type of trans-resveratrol is best? Does it have to be organic etc...? 2 Peter S Peter S 2 years ago Do you think that people supplementing with NAD+ or NMN should also take TMG to avoid methyl depletion? Another youtuber recommended that and I think a lot of people are doing that so it would be great to hear your thoughts on it. Larry Tate Larry Tate 2 years ago Dr. Patrick thank you for sharing your knowledge. Could you please consider doing a video lecture on CBD and it’s benefits and possible negatives? Thank you Steve Wright Steve Wright 2 years ago Many thanks for this detailed walk through! I think correctly identified this is all at an early stage of discovery - perhaps usefully highlighting the key importance of NAD in the cell energy economy, but (to my mind) now pointing to the need to reduce NAD depletion. A whole food plant based (WFPB) diet being inherently low inflammation - perhaps best indicated by White Blood Cell count: https://nutritionfacts.org/video/what-is-the-ideal-white-blood-cell-count/ Also points to a need to remove senescent cells regularly - probably by a number of 5 day water fasts over the year. Lastly - not a bad shout to pay attention to Vit B3 status - if this is insufficient NAD production is likely to be throttled... Thanks Rhonda for this review - appreciated! a2audrey a2audrey 2 years ago I’ve been taking 750mg of NMN + 500mg resveratrol for 6 months. I’m 61 and in good, but far from great shape. My muscle stamina and overall energy are way higher than when I began. I also have noticeable reduction in inflammation in my leg that was damaged in an accident and where it had been chronic for many years. The reduction in inflammation alone keeps me on it, but feeling a lot better is great too. 42 Daniel Gutierrez Daniel Gutierrez 1 month ago Rhonda, fantastic video and quick important question: what about taking NADH as an oral supplement? Would that damage the NAD+/NADH ratio? Producing negative "Old People Ratio" effects? Hemi Dave Hemi Dave 1 year ago Agreed....They need more study on NR and NMN to see if existing cancer cells will also "benefit" from it. You have natural options to raise NAD: Fasting, Low Calorie Intake and Exercise. pravoslavnik pravoslavnik 2 years ago Just found your channel/videos. Your presentations are "spot-on," as the modern expression has it. Loaded with information. Love your vids. I shall be watching them all. PLEASE keep up the good work ! 2 FoundMyFitness Konstantin Yavrichev Konstantin Yavrichev 1 year ago Congratulations 🎊 - It's pretty rare to be able to see such good quality presentation for so complex topic and compressed in so condensed time 1 Ken Otwell Ken Otwell 2 years ago So what's causing the nicotinamide feedback loop and how do we increase the NAD+ setpoint so that more nicotinamide is converted? That seems to be the key to increased usable NAD+ in the body. jackwelshh100 jackwelshh100 2 years ago (edited) Very interesting episode. Fantastic work! One question comes to mind: We know that resveratrol is a SIRT-1 activator but are there identified compounds that increase PARP-1 activation (for us with non-supercentenarian genes)? I supplement with NR and resvetral. If there's a PARP-1 specific activator compound, it might be an interesting addition to the mix. 4 Trey IM2 Trey IM2 2 years ago I had literally finished my bottle of NAD3 the day before this vid dropped. Took my last TMG (DEFINITELY take this with it for methylation. I also take an activated B complex for additional methylation regularly) this morning (skipped yesterday, and I think I took an extra NAD3 by accident so it wasn't 1:1). I had been on this for 4 months straight after taking a month or so off with a 2 or 3 month straight intake prior. I don't know if it improved anything because I take a ton of supplements and herbs to improve my overall health. I plan to take a month or 2 9ff before getting back on NAD3 Christina Lawhorn Christina Lawhorn 2 years ago ** Due to a serious case of mold poisoning I have been on NAD+ IV's for 6 months... they literally saved my life.. I have never had oral NMN or NR. However I would highly recommend anyone with a health issue try NAD+ IV's. There are numerous accounts of people turning their life around due to NAD IV's not precursors. Best wishes to each of you! 1 Aldous Orwell Aldous Orwell 2 years ago Beautiful! Very comprehensive! Question: does DNA repair have a circadian rhythm? If so, would it be better to take NR in the evenings? Thank you! :-) B Szlado B Szlado 2 years ago Great summary of the topic! I was desperately trying to find actual human published data about NAD+ decreasing with age, but other then the two studies you mentioned, couldn't find much. In those two studies though, sertuin activity actually didn't decrease; there was significant difference between men and women, and the NAD+ decrease was extremely small (vs. the 50% David Sinclair commonly mentions), and in fact looking at the graph, it was quite variable, with some 30 year olds having the same level as 60+.. .so I really wonder if the marked "decrease" in NAD+ everybody goes on and on about is actually a comparison of an active 20 year old vs. a sedentary, unhealthy 50-60 year old; ie. isn't the life style more important than the horrendously expensive NMN and NR supplements? 2 Gregory Colvin-Garcia Gregory Colvin-Garcia 1 year ago Rhonda, I've heard Dr. Sinclair say he takes 1gram of NMN daily but in reading his book and listening to multiple interviews I'm not sure on the best time metabolically to dose and if there is a "minimum effective" single dose. Currently, I take 250 mg, 3 to 4 times per day sublingually but it occurred to me that each one of these single doses may be below the threshold of effectiveness... 2 C H C H 2 years ago (edited) Regularly growing broccoli sprouts for a couple months now, I put them in my daily smoothies for myself and my family thanks to Dr. Rhonda Patrick. Thank you for all this free info, truly is life changing 36 FoundMyFitness Ashlee Wicklund Ashlee Wicklund 2 years ago Is it possible to share a video of your general diet? Also how do you deal with extended family when they offer food at any occasion, birthdays, holidays, or just family meals. I also have a kid who is more influenced by my families bad eating habits but I can’t necessarily control everything that she eats when I’m gone? Your thoughts on the matter would be greatly appreciated! Lastly, is there a balance to this way of life? If I’m going strong for with eating well and then eat some chocolate does all my hard work go to waste or is that okay to do once in awhile? Rick Kowalchuk Rick Kowalchuk 2 years ago It seems to be a key component involved in aging and possibly reversing the process! Dr. Steve Hruby, D.C. Dr. Steve Hruby, D.C. 2 years ago Thank you Dr Patrick this is above and beyond anything that is out there. 1 FoundMyFitness SmartCookiesDontCrumble SmartCookiesDontCrumble 2 years ago Thank you so much for giving this teaching on this topic. Valuable information!🥰 2 David Alzate David Alzate 11 months ago I guess in the section on depletion, it's important to include the studies of CD38, CD157, and PARP. These molecules are impacting the depletion of NAD+. Equs Narnd Equs Narnd 2 years ago I'd like to see you do a video on the role of Niacin (Nicotinic acid) on supplying the molecules for the manufacture of NAD+ 11 Dave Soko Dave Soko 1 year ago Can you boost your NAD levels by taking Niacin or do you have to use the other forms such as nicotinamide riboside or are they the same thing? 1 John Hunt John Hunt 2 years ago Thank you . Please do not stop helping people the way you do. 1 Jeff G Jeff G 2 years ago Great video. Now I'm wondering how long it will be until we have solid data on positive effects as well as dosage sizes and methods to experience those effects. Years? How many? carpe diem jonah carpe diem jonah 2 years ago (edited) So what are the best relative food sources of NMN? And the best relative food sources of Nicotinamide Riboside? Are some of these foods significantly higher in their capacity to boost NAD levels? Hoping to get it aboard the food supply so there is the safety of letting the body metabolize as needed. Entropy Wins Entropy Wins 2 years ago I'd be interested in a video on pterostilbene and thoughts on supplementing that. Optimize with Science Optimize with Science 2 years ago It is a fascinating topic and I love how scientific Dr. Patrick approaches it! 41 FoundMyFitness Launch Medical Media Launch Medical Media 2 years ago This is a wonderful video. We will probably be sending our patients this video when they ask about NAD+ from now on! NADIOL™ NADIOL™ 11 months ago Amazing content as always Rhonda. Josh Woodle Josh Woodle 2 years ago (edited) Dr. Rhonda, since Dr. Jed's video, I've been eating just the broccoli seeds. Is there any way you could do a video on consuming broccoli seeds vs. sprouts? I burr grind them and sprinkle over food. Very interested in this! Ron RG Ron RG 2 years ago Dr. Rhonda amazingly insightful analysis of the data! Love the topics and videos you make . Thank you so much 1 Thomas 3108 Thomas 3108 2 years ago Hello, I have one problem taking NMN. Hope you can help with your knowledge 😃🙏 I take 0,5 - 1 gramm Resveratrol in Oliveoil, 0,5 gramm of NMN and 1 gramm of TMG. I started taking TMG as I noticed, that after about 3 days I feel a certain pressure on my heart or let's say on the left side of the breast and I feel like having higher blood pressure. Then I stop the life extension treatment and everything is fine again. When I take only Resveratrol or only TMG this phenomenon doesn't appear. I thought with taking TMG this would stop, but that's not the case. After starting again the same reaction appears. I bought NMN even from Prohealth. The same thing. The dose is really not that high, so I am wondering as the studies don't have such results. I am 45, 1,75 m and about 83 kg. So what's your opinion on that? Thanks in advance, Thomas 1 Isabella Holden Isabella Holden 2 years ago Amazing video. Thank you for the brilliantly explained content.🇿🇦😎🙏 5 FoundMyFitness Waskel E. Wabbit Waskel E. Wabbit 1 year ago (edited) I have been taking 300 mg a day of NAD+ for almost a month. Took about three weeks before I started noticing a difference. It is very subtle but that might be a good thing. There's a fine line between feeling like doing something or not. I have had chronic fatigue for all of my adult life. I am 68 now. Been looking for the magic pill for that long. Getting ready to order my second bottle and I would recommend to anyone with low energy levels or just to possibly live longer. No one I've talked to has heard of this stuff. Would like to get some friends to try it to see if it helps them too. 1 Casey Connor Casey Connor 2 years ago Fantastic as usual! Thanks for the great information! Hey, if you get a chance to circle back on the subject of eating fresh ground broccoli seeds at some point that would be great. Many of us like the taste of them! Or don't notice it when added to food! Erucic acid might be a consideration? Thanks! 2 AJ Hege AJ Hege 2 years ago Appreciate the info! What are your findings about NADH (NAD bonded with a hydrogen ion)? I hear it has a better delivery system from a doctor on the Ben Greenfield podcast. Robert C Robert C 1 year ago Is NADH taken sub-lingually an effective NAD booster ? What is your opinion regarding the risk of cancer from taking it ? As for dose, is 20mg a good dose for an 85kg adult male ? Thank you in advance for your advice. Murray Woods Murray Woods 2 years ago TY Dr P. Two questions. 1. Can shilajit increase bioavailabilty of NAD boosters ? 2. How much trans pterostilbene are we talking about ? I believe there is a little bit of that in the blueberry extract contained in my daily Lef Mix powder supplement. I take 200mg of NAD/dy. Dr. Michael Moeller Dr. Michael Moeller 2 years ago I personally get NAD IVs@24:30 and use them with my patients and notice an increase in energy and improved cognition. 31 Dan O'Hanlon Dan O'Hanlon 1 year ago Very helpful — comprehensive — much appreciated! 2 Erin Greer Erin Greer 2 years ago Thanks for so much important information. I hope more studies in humans are coming. mintsaturn mintsaturn 7 months ago This is interesting to me bc I saw our local drip bar does NAD+ infusions. My mom gave me some nicotinamide adenine dinucleotide vitamins and was like "Oh this is the same stuff" Obviously it's not. Peter Davila Peter Davila 4 months ago (edited) I'm 66 years old. I started taking 1g a day of Apigenin a few months ago. Apigenin is one of the ways to raise NAD+. It is amazing how much energy I have now. It feels like the energy that I remember having in my 30s. In addition, in conversation with my neighbors, they have commented that I seem to look younger every year, instead of older. I also take Metformin, even though I do not have diabetes. I also do a lot of intermittent, 3 day water fasts. I'm planning to start on 1g of Fisetin a day for 5 straight days every month in the next few weeks. My diet is free of meat. I do eat sardines, salmon and flounder. Lots of nuts (Walnuts, Cashews), seeds (Flax, Hemp, Chia). Dark Chocolate (80% cocoa). I do drink lots of coffee. I don't drink cow milk but almond milk instead. I don't drink alcohol. Live Long Enough to Live Foreever. 1 Serafino Anselmo Serafino Anselmo 7 months ago How long does niacin (b3) take to lower your calcium score? Days, weeks or months? Anh Nguyen Anh Nguyen 2 years ago Thank you for such an educational clip . Please keep it up 3 rzomg rzomg 2 years ago Amazing skills to flood my brain with huge amounts of information. I know how its gonna feel when AI's are smarter than us all its the same feeling I get when I listen to Dr.Rhonda 2 Ed Mistarka Ed Mistarka 2 years ago Rhonda can you do a review of these two different approaches to longevity, one using 6 herbs , most that work synergistically and by different pathways, and also work synergistically with spermidine and resveratrol. The other is a Horvath publication from last September that utilizes recombinant growth hormone combined with metformin and DHEA to try and awaken dormant thymus glands in the elderly, and discovered, by surprise, major improvements in prostate cancer markers, ( like LMR ) and also it did wake up the thymus gland from dormancy. The 2019 Canadian publication "Pairwise combinations of chemical compounds that delay yeast chronological aging through different signaling pathways display synergistic effects on the extent of aging delay": http://www.oncotarget.com/index.php?journal=oncotarget&page=arti... The Horvath publication on jump starting the thymus gland "Reversal of epigenetic aging and immunosenescent trends in humans" ( https://onlinelibrary.wiley.com/doi/full/10.1111/acel.13028 ) Grant Ross Grant Ross 1 year ago I had NAD infusion today-actually trialing it before offering my patients. Very interesting comment on Nicotinamide and its feedback loop relation to Sirtuin. I would be curious to hear comments on how this might relate to the use of nicotinamide in non-melanoma skin cancer prevention. Kunverji Hirani Kunverji Hirani 1 year ago Hi Rhonda :-) So practically, what do you recommend? Take NMN first thing in the morning? - then how long should you wait before having breakfast? Or can you have breakfast straight away after taking NMN? Many thanks :-) Vijai shankar Srivastava Vijai shankar Srivastava 5 months ago Highli informative video based on different experiments on NMN AND NR. It’s doses n application well covered. mooky wilson mooky wilson 2 years ago DOC!!!! your the best! I wish there were more docs like you. 4 Plaebian Plaebian 1 year ago I think you can't make more concise and comprehensible than this 1 Ryan Mckenna Ryan Mckenna 1 year ago A Great presentation and very informative. This research sounds promising. 1 N C N C 1 year ago Have there been any recent studies on whether or not NR can or cannot cause tumors or cancer? Tom Wallace Tom Wallace 2 years ago Heard the podcast, but this was much easier to digest. big question for me is how much does intermittent fasting, time restricted eating, long-term fasting and exercise increase the NAD+ in your cells? Does, say a 18 or 20 hour fasting window to the trick?. Leslie Porter Leslie Porter 3 months ago That was a very thorough explanation Dr. Patrick. Thank you. firehorse_44 alpha-omega firehorse_44 alpha-omega 2 years ago Excellent lecture. Thank you for this presentation Dr. Cheers 10 Eric Storvick Eric Storvick 2 years ago Any thought on blocking NAD degradation w/ ubiquinol and/or apigenin (dried parsley)? Gunasheelan Suresh Gunasheelan Suresh 2 years ago Hello Rhonda....Your work is much appreciated...Please do a video on how to overcome psoriasis... Thanks Hit Hit 1 year ago Hey Rhonda, does taking 1g of resveratrol trans matter if you aren't taking NMN And does resveratrol work with NAD+? From my understanding NMN gets converted to NAD+ with resveratrol. Mr.GreenGold Mr.GreenGold 2 years ago Rhonda please talk about Inositol Hexanicotine and L-Tryptophan's role in raising NAD+ levels. Chad Aitken Chad Aitken 2 years ago Thanks for the effort on the video. Can anyone get the Parp-1 activity level test? Do we know what happens in the Salvage pathway recycle that lowers levels as we age? I seen reports that % gains is not good enough in older people since it’s so low? Jason Humm Jason Humm 2 years ago I've been taking 1g of NR daily for 2 years. I find that I don't get jet lag or hangovers anymore. My contribution to this fascinating science. 16 Spencer Davis Spencer Davis 2 years ago thanks Dr Rhonda. great video. have you tried blue infusion of NAD+ yet? some people try it and claim it works feeling better with more energy but some of them also say it is very uncomfortable. I will probably try it sometime just want to know more about it. Also, what are your thoughts on clinics like Dr Neal Riroridin having the stem cell transfusions such given to Mel Gibson's father which seemed to have a positive effect. It would be a great interview for you. thanks Oh and btw you look beautiful and super vibrant! Paulie Walnuts Paulie Walnuts 2 years ago Surprised you’re not doing NMN with all the positive research out there. I’ve been on NMN/resveratrol protocol since April 4, 2019 and feel superb. Also just recently went vegan just for fun for a month and because my body felt terrific, extended another month and May just stick with it. Dropped 15 pounds w vegan in one month. I do between 250-500MG per day of NMN. 1 dikeledi mogobe dikeledi mogobe 1 year ago Hi Dr. I started NMN regime last month and yesterday I started seeing some small images like a very small moving object and yet there was none. Maybe you can assist me as what could be causing this. I take 1g NMN, 1g Resveratrol , 800g Fisetin,200g Hyaluronic Acid , 1g TMG, Vit K2, Vit B12, Vit C Zinc and selenium Jack Brown Jack Brown 2 years ago Great video. Would you be as kind as to consider making a review of studies on NADH supplementation ? 1 Peter Peter 1 year ago Thank you. I'd like to know more about the supposed SIRT1 inhibition by nicotinamide. Seems critical to get this untangled as it is a key precursor to NAD+. Many nicotinamide supplements that are 10 to 40 times RDA. What's that about? Conversion? Noemí Romero Noemí Romero 2 years ago You are amazing!!! This material is so useful! 12 FoundMyFitness Stephen Hurst Stephen Hurst 2 years ago Thank you so much for the thorough long-form video, all of this information is very important. 1 Rick McGhee Rick McGhee 1 year ago Holy crap, this was amazing. This 30 minute video took me 2 hours to get through. Had to pause and read and then pause and research. Thank you!!! 1 Richard Wheeler Richard Wheeler 2 years ago Best presentation on the NAD/NMN potential and current status I have seen anywhere. N C N C 2 years ago I've been doing lots of reading and this is by far the most in depth breakdown I've seen on NR/NMN yet. While I am really impressed with anecdotal evidence of NAD supplements, I am erring on the side of caution. Personally, I'm going to hold off on purchasing supplements until more clinical trials come out so we know proper dosages and whether or not this even carries over from mice to humans. Anyone else feel the same way? If it turns out this is safe, easily replicates in humans etc then I will consider supplementing. 2 valar valar 1 year ago NAD+ is a miracle drug and no mistake. The hype is real. It has had a profound effect on my mental and physical health at the age of 41, after many years of drug abuse, persistent old injuries, and the general exhaustion brought on by 25 years of OCD and sky-high anxiety. 2 Mathew Andresen Mathew Andresen 2 years ago Very interesting as always. I do note though that Dr David Sinclair seems to be having some pretty good results with his personal supplementation of 1g a day of NMN 10 carpe diem jonah carpe diem jonah 2 years ago (edited) In your past videos, you have also brought out the benefit of dry sauna. In those videos the benefits gained from exercise, calorie restriction, and fasting were also seen in dry sauna use. So, does dry sauna have a beneficial effect upon NAD levels? The hormesis effect? 1 Nathan Nathan 2 years ago Great video, thank you for quality information! 2 Misses Witch Misses Witch 1 year ago I do wonder how it effects those on HRT who take hormones for other medical conditions. Would the NAD+ negate its effect? I say this because I tried NAD+ and had many side effects, Showing the symptoms I had before I was on the HRT medication. Bill B Bill B 2 years ago Awesome vid. Is there a dose at which oral nicotinamide can boost NAD recycling while not suppressing sirtuin activation? Lauren Luzod Lauren Luzod 2 years ago Dr Rhonda, the section regarding the possible formation and progression of tumor cells in rats from the study you sited was alarming to me because I am currently taking 1 gram per day of NMN... As well I am sure many of your other followers are taking NMN. Do you have any further comment on this. or can anyone else elaborate on this from others studies.. as this study I believe was about 1 year ago. HappyTirade HappyTirade 2 years ago What a masterpiece! Thank you! 3 FoundMyFitness japrogramer japrogramer 2 years ago I've always suspected NAD as a fuel source for cancer since it promotes glycolysis .. is there a quick way to measure nad like a blood meter for glucose? TheGrasspond TheGrasspond 2 years ago You are absolutely awesome!! This was very helpful. Thank you 1 R R 2 years ago Hii rhonda.. very intellectual and useful content for improving health...but now I am going to say..there is an important science in India named Ayurveda...which had told the method of diet and regimen 5000yrs ago...it came from Vedas of indian culture one of the oldest books written in the world...the maharshis ( saints )in India have gained the knowledge of Ayurveda which includes the yoga and rasayanas ( rejuvenation therapies ) long time ago from lord dhanvantari...regarded as father of Ayurveda... Ok.. now the point of telling this information is that I found that u have excellent knowledge over genetics and anti aging mechanism.. U have to notice a point that Ayurveda had told healthy ( anti aging ) diet and regimen 5000yrs ago without any equipment or machinery... Now the world have understood the science behind the yoga and it's benefit...but actually it's a small part of Ayurveda but a lot more to know about the science of living ( Ayurveda ) My name is Rahul.. from india...my request is u to gain knowledge over Ayurvedic science ... and I am warning u if u miss to know this science u r missing a crucial knowledge over science of life... This is my humble request to make a knowledge and implement for better health and longevity.. Thanks. Sam W Sam W 10 months ago This is an excellent summary of the literature. Thankyou Super Heroes Super Heroes 2 years ago I started taking NR from Niagen using their 300mg packets 2 days ago and I’m 18. I’m so excited for what this can do! Soon I’m going to be surfing with a good friend and now I’m ecstatic that I might be able to live a lot longer and surf with him even longer too 1 Mr. Reberdo Mr. Reberdo 2 years ago Best video I’ve seen on the subject. Thank you!! 4 Mike Breler Mike Breler 1 year ago I don't like the idea of intravenous injecting myself but I would have no hesitation with subcutaneous which I have done with B12. Is there any data on tissue absorption of NMN or NAD, of say 500mg, dissolved in saline with humans considering that it is bypassing the digestive tract? Thanks Ro Bo Ro Bo 2 years ago Wow talk about informative video thank you , I’ve been taking a product which contains Nicotinamide 250 mg twice a day with botanical mix alpha lipoic acid vitamin c and zinc which apparently works on the salvage pathway you have described. The company claim that Despite all the hype surrounding the use of precursors such as NR and NMN to boost NAD+, our cells actually make nearly all of the NAD+ they need using NAM ,as you described in your presentation. They go on to say That “as we get older, levels of the NAMPT enzyme are known to decline and the salvage pathway becomes inefficient at converting NAM into NAD+. In fact this is known to be one of the main reasons that cellular NAD+ declines as we get older. This means that if we want to make sure that NAM doesn’t build up in the cell, then we must make sure that NAMPT and the salvage pathway are working optimally, like they were in young cells. Rather than simply trying to fix falling NAD+ levels by putting more raw material into the cell (using precursors) the product is designed to fix the underlying reasons why NAD+ is actually declining, namely the failing salvage pathway. I am however concerned of the effect of Nicotinamide on sirt2, they claim however that Nicotinamide does not effect sirtuins if it doesn’t hang around for a long time when the salvage pathway is working properly . I just wondered what your thoughts are on this. Again many thanks for your very detailed video 2 Bart Bart 2 years ago Thanks for the good video! I was wondering where is the source for the statement that NAD is lower in older human muscle compared to young. Does this data exist? 2 Aston Fallen Aston Fallen 2 years ago Thanx Rhonda, for an excellent presentation ! I've heard about this mainly from David Sinclair - I am following closely, and I look forward to more input from you ! kineticx kineticx 1 year ago Awesome, best NAD video so far, you saved days of research in such information dense video, awesome work justin han justin han 2 years ago Very good and objective presentation from Dr. Patrick about NMN supplement, unlike Dr. Sinclair, who seems just want to promote and present NMN information in a selective way. 3 David Schwartz David Schwartz 2 years ago Been looking into this a bit recently. Thanks for the new upload, Rhonda! 👍 1 Maya Maya 11 months ago Was there any information on if you took a supplement(nicotinamide riboside),when would be beneficial(timing)? Morning, evening, after a workout, with or without food, not with certain foods or medications? Don Castella Don Castella 2 years ago I would like to hear a discussion of Nicotinomide Riboside and its effect on Glutathione levels. Is Tri Methyl Glycine supplement needed with NR? Beaker Beaker 6 months ago well been taking 300mg /day of NR. I have spinal osteoarthritis and wake up with horribly sore spine every morning. Days after starting this, I woke up with zero back pain and have been pain free since. I don't think it was placebo because I thought it would take 4-6 weeks of supping and I never thought it might help my morning pain, just maybe help me age more gracefully. So, for me.. long term risk could be a possibility, but I will take this risk to wake up pain free everyday. CompSource Inc. CompSource Inc. 2 years ago Wow amazing job and the best, most concise source of information I've ever seen for NAD oh, great job! 1 Creighton Freeman Creighton Freeman 2 years ago It sounds like senolytics should be taken with NAD+ boosters to mitigate the potential for renegade senescent pancreatic cells. David Sinclair also mentioned that Niacinamide inhibits Sirtuins, but since it is also converted to NAD+ that didn't make sense to me. The saturated conversion pathway may explain that, but then the question becomes at what dosage of Niacinamide does the pathway reach saturation? At least one publication by Hwang and Song, ("Nicotinamide is an inhibitor of SIRT1 in vitro, but can be a stimulator in cells." Cell Mol Life Sci. 2017 Sep;74(18) ) suggests that since Niacinamide is rapidly converted to NAD+ in cells it only temporarily inhibits Sirtuins followed by an increase in NAD+ and increase in Sirtuin activity. If this interpretation is correct it opens the possibility of using a much less expensive compound as an NAD+ booster and Sirtuin activator. Do you have any additional information or thoughts on the potential of Niacinamide as an NAD+ booster? 5 Sirt1 Sirt1 2 years ago Can you supplement with NADH directly? If so, how much mg is considered safe and effective? Luc Marcoux Luc Marcoux 1 year ago Wow, thank you for that full explanation. I have cut sugars, am into intermittent fasting, with 4 prolong 5day fast a year, and now looking into Mr Sinclair's research. I don't look anywhere my age, I'm told. Exercise and eating right is crucial. I am a nut at getting my DNA and any acetylation or methylation repairs done. Doing the autophagy route for this right now. I was thinking of these surpluses, but I think, I'll stick with my diet and exercise routine. Thank again, great explanation, backed with studies. I'm loving you already! 1 Arturo Gutierrez Arturo Gutierrez 2 years ago Excellent presentation, thanks a lot! 1 Ancient Gains Ancient Gains 2 years ago Is there a means of testing NAD+ levels to confirm if oral NMN supplementation is effective? Mitesh Patel Mitesh Patel 2 years ago I think you missed Niacin, which is more potent NAD booster than NAM, NR or NMN. I think the trammel study compared all 4 for their role as NAD boosters. B K B K 2 years ago Amazing video, thanks for the in depth overview! I've been following NMN for a while now, and this is the best video I've seen to date, covering every aspect, concisely. Absolutely amazing. Now if only supplimenting NMN wasn't so cost prohibitive. 😅 I'm up for being a long term study if anyone needs a test case. 🤔 I've got about two years worth of detailed food logs, heart rate, sleep logs, weigh ins, blood work, exercise, and the likes, that I'm farming up anyway as part of my commitment to getting fit. I wouldn't mind sharing with actual doctors and scientists. 😋 🤷‍♂️ Either way, thanks for the awesome info! GLHFDD! ✌️😈 7 MrVeteranhawk MrVeteranhawk 9 months ago I did 10 push ups for every time she said NR or NMN, I don't have to workout until the year 2023 now..lol. But great presentation, very helpful for my age at 52. I've been doing a lot of research on taking supplements to increase NAD+ 1 Shaji George Shaji George 2 years ago Hi Dr Patrick: Very interesting presentation. In light of the data that you showed that there is a real possibility that NMN and NR are converted into NAM and recycled in the salvage pathway to make NAD, why not consume Nicotinic acid? The recent paper in Cell metabolism showed that NA supplementation in humans with mitochondrial myopathy in a small clinical study showed 2.3 fold increase in muscle NAD in the patients (not in healthy controls) and an 8 fold increase in blood NAD in these patients (5 fold in controls) at 1 g/day over 10 month (escalating dose from 250 mg to 1 g to reduce flushing even though they used a slow-release version of NA). This is much more of an NAD boost than NR or NMN which probably supports the theory (from the metabolic flux study you mentioned) that NMN and NR are converted into NAM and cycled through the salvage pathway thereby negating the advantage of NR and NMN. However, the authors studied only muscle and blood, so it is not clear if NAD levels in other tissues also increased. Other studies have shown that IV administration of NAD can increase NAD levels in brain. So if blood NMN can be raised 5 or 8 fold, then it can cross into the brain (If I remember right, there is some data to show NAD crossing BB barrier although I am if more studies will show it through the NAM route). NA is very cheap and has good safety profile (having been used in high doses as an anti-cholesterol agent in the past). Slow release NA can ameliorate some of the flushing even though some suggest based that the slow release form may cause liver toxicity (very limited data). Would like to hear your thoughts on using NA instead of NR or NMN. CLAY STONE CLAY STONE 1 year ago Thank you Rhonda I will watch this again you explain NAD+, NR and NMN excellent easy to follow. I will come back to this video on referencing one of the best out there. God Bless you. Djavan Carvalho Djavan Carvalho 2 years ago I’ve been taking 500mg of NMN for at least 8 months idk if this dosage affects my body but at least makes me dream for days, and sleep really well, as well steady energy trough out my day. Joshua Joshua 1 year ago (edited) You satisfied my curiosity about what NAD+ is, as I first saw the NAD+ supplement in my health food store. A company called Life Extension produces it capsules, and I have already been taking their CoQ10 and Curcumin Extract. I'm going to buy it next time. This is a link to their NAD+ product. I do not represent the company. https://www.lifeextension.com/vitamins-supplements/item01904/nad-cell-regenerator?msclkid=7d88b03d6e7d1b66d163ef8957eecfce&utm_source=bing&utm_medium=cpc&utm_campaign=LEF-SHOP-EN-BRAND-US-Bing&utm_term=4583382945806338&utm_content=All+Products+Brand Formerlyknownasnone Formerlyknownasnone 9 months ago (edited) Thank you for what you do. When you say NAD can be made from things in the diet like tryptophan and niacin equivalents what do you mean? Serine? Other amino acids? Tom Jones Tom Jones 1 year ago I like to watch informational videos like this while doing my hour of piano warm ups. Nope....can't do it on this one. Need to give this video my 100% attention. Thanks Dr. Patrick. 1 Billy Huggins Billy Huggins 2 years ago Thank you, Dr. Rhonda Patrick! Great information! 2 Paula Feener Paula Feener 2 years ago Can you please talk about methylation in association with NR supplementation and how to mitigate these effects ? Sunshine HappyFunTimes Sunshine HappyFunTimes 2 years ago Thank you for being honest and thorough. You are the best Leoji67 Leoji67 2 years ago Are there any studies that look at pre and post fasting levels? Is this the key factor in the relationship between fasting and longevity? 3 Joey Koningsbruggen Joey Koningsbruggen 2 years ago Thank you! This is wonderfully insightful. 2 Hans Hags Hans Hags 2 years ago Thanks Dr. Rhonda Patrick, great video on this rather new topic, also enjoyed your interview with Dr. David Sinclair some months ago, great job... I'm trying out 500mg NMN daily, along with 500mg Resveratrol and 500mg Metformin (recovering Type 2 diabetic ;-) Off all other meds incl. high doses of insulin, on Keto last 8 months (108 kg down from 126 kg), and doing OMAD in a very satisfactory fashion ;-) I'm thinking to follow Dr. David Sinclairs suggestions for better health and life longevity, but agree, it's most likely very difficult to make any long term conclusion yet on health benefits, but I'm hopefull and optimistic moving forward (I'm 51 years old) ... also looking into probiotics now, and fasting, as well as following the Ketogenic lifestyle. So, I'm thinking, shouldn't we all just embrace NMN and/or NR for now, and to get started to boost NAD levels best possible ;-) ... and untill Dr. David Sinclair comes along with something better ;-) Again, thanks for informative video! 1 Falcon77 Falcon77 2 years ago Awesome job Rhonda, great info. I’ve been on NR 300mg and pure resveratrol for 8 months now Misel982001 Misel982001 1 year ago Excellent video. Well done girl. echoman echoman echoman echoman 2 years ago The best all-in-one-video on Youtube that explains NAD+ Peter Wu Peter Wu 2 years ago I'm exactly 180lb, thank you for this vid! 3 JustASimpleGuy JustASimpleGuy 2 years ago (edited) I experimented with both NMN & NR for several months, however the potential risks and unknowns taking the high dosages needed to facilitate the stated anti-aging goals just don't seem worth it. Not to mention I did notice a slight performance decrease in resistance training after several weeks on both. I'll stick with resveratrol and a few critical mineral supplements, low carb diet of whole foods rich in healthy fats, anti-oxidants, sterols, etc... prepared at home and free of sugar, weekly 24-36 hour fasting, resistance, aerobic and HIIT training, meditation and a good amount of outdoor activities in nature, the wilder the better. ⵣ=ⵣ -ⵣ-=-ⵏⵉ ⵣ=ⵣ -ⵣ-=-ⵏⵉ 2 years ago thanks for sharing ..after taking it for about month , so far I noticed improvements in endurance (running, sparring) . and as a guitarist it helped me increase my picking hand fingers endurance when playing long trains of trills and tremolos...the bottom line, for me it works for endurance and energy . Mister Sir Mister Sir 1 year ago (edited) Excess nicotinamide needs to be methylated in order to be catabolized and excreted, which increases the body's demand for choline or other methyl donors (10:48 bottom right). FYI If I'm not mistaken you need 28mg choline to methylate 100mg niacin. John Schlick John Schlick 2 years ago I really would love to know the reference for changing mg/kg from rodent to human equivalent... Can anyone point me at a paper that describes how they calculated this factor? (I've looked for it a couple of times and never found anything.) armadillotoe armadillotoe 2 years ago (edited) I really wish you had been my instructor in my Biochemistry class. You would have had my complete attention. You have a gift for teaching. I have broccoli sprout powder, and broccoli sprout seeds on order. Thank you for the work you have done, and for making it available to everyone who is interested. native Texan native Texan 2 years ago (edited) Seems Fasting, calorie restriction and exercising is about the only thing that works for the moment. No magic pill. Great information 👍 72 William Tomkiel William Tomkiel 2 years ago TY for a valuable comprehensive explanation combined with 'perspectives" from Dr. David Sterling and his self dosages from 1 of his videos, 5 grams/day of NAD+, iirc maybe a more intelligent eating. sleeping, stress reducing life style is a better answer than "more is better' JohnnyBoooy 01 JohnnyBoooy 01 2 years ago Could Piperine in black pepper allow a great bioavailability thus getting NAD+ further into the body? Mike Breler Mike Breler 2 years ago At your young age and optimally functioning brain, you can probably wait another 5 to 10 years for more definitive data before worrying about your NAD levels. Since I'm an older mouse, I'll take my chances that at about 500mg of NMN per day may end up as expensive urine. 2 ÆRO ŠPECIALIST ÆRO ŠPECIALIST 11 months ago Absolutely GENIUS. Thanks ❤️ Sean Romanowski Sean Romanowski 2 years ago Thanks doc! Big inspiration to me MOONOOM MOONOOM 2 years ago Please Rhonda, the Floxing community needs you to make a video about Fluoroquinolone Toxicity! 16 scott crabtree scott crabtree 8 months ago Thank You for all this outstanding information. Peggy Halsey Peggy Halsey 2 years ago Off topic but would you consider making a very short video on food and supplements you suggest in pregnancy. Less than 5 minutes and applicable to lower education levels? ACURA4LIFE ACURA4LIFE 2 years ago Part 2 on the doses please Rui-9-CS Rui-9-CS 2 years ago I have a quick question, I am taking Niacin in the morning, and don't bother by the flush, but my mother is very sensitive to the flushing, so I bought her a bottle of Inositol nicotinate (non-flushing niacin), I was wondering does Inositol nicotinate raise NAD level+ level as well? Jon Hylow Jon Hylow 1 year ago If I intend to take 100 mg NAD + per day, how much TMG powder should I add to my diet ? or should I consume the raw version of NAD instead ? G G 2 years ago We took 125mg NAD through pregnancy and lactation with breaks every 2 weeks for a few days. Our daughter is exceptional, mentally and physically. She heals from infections and skin cuts like a mutant. In addition to NAD we took a host of supplements I deemed safe by NCBI study reviews 8 DDFaye74 DDFaye74 1 year ago This is just about the most thorough and intelligent PP presentation I've ever seen. She is one smart lady! Wow. Brogan Josh Brogan Josh 1 year ago What is the difference between Nicotinamide riboside vs Nicotinamide Mononucleotide? William Read William Read 2 years ago (edited) It increases the growth of brain cancer and glioblastoma tumors in some studies I read. 1 ColdHawk ColdHawk 2 years ago NAD+ sounds like it will lead to the next great fitness FAD+ 1 Carroll Hoagland Carroll Hoagland 2 years ago (edited) NAD is part of a complex system for growth and still having how it works to benefit health. Another good discussion by the CarnivoreMD, Paul Saladino and David Sinclair (Sirtuins) ... a Carnivore and a Plant based diet proponent .. but highly technical, to include NAD- https://www.youtube.com/watch?v=qJV-SdaRHvA Ken Otwell Ken Otwell 2 years ago Great data - but leaves me with one big question - how much does exercise increase NAD+ and how does that compare with supplementation? Also - is there any synergy between exercise and supplementation? 65 ManOfEck 1812 ManOfEck 1812 2 years ago (edited) In the early 2000's they dug up a famous Roman Gladiator. I don't remember his name. They found that his bones were bigger and he was drastically more healthy than we are today. They dug up other Gladiators as well. They found out that, collectively, their bones, at some point during training became more dense. The carrier Gladiators bones had rings in them, like a tree. The study found it was diet and training. My 2 cents 🍺 1 am am 6 months ago I've seen some research suggesting a link between NMN and enhancement of abnormal cell replication. David A David A 2 years ago how about absorption and bio-availability of liposomal form of NAD+? and also when NAD boosters get converted into NAM, does the latter deactivate sirts? or it only does when NAM(or niacin) is taken orally? Thanks J K J K 2 years ago Excellent Analysis - Thank you amber skies amber skies 5 months ago I have been saying this... longevity is our choice! If we want to sustain our health, we have to do something about it! This will start by knowing what supplies our body needs. Start by eating a balanced diet, quit vices, exercise regularly and take an NMN supplement. Mine is a 500mg per day, NMN by Vitruvin. I just knew all of this lifespan information through Dr. Sinclair's book. It says there's no point increasing lifespan without. The goal should be to live longer while staying young and healthy. Sinclair believes that in the foreseeable future, it's possible for humans to live beyond 120 or even 150, without the frailty, diseases and disability we now associate with old age. See your result and tell the same experience. Michelle Guerrero Michelle Guerrero 2 years ago Dr. talks to us like we could really get it....I do!!! 15 FoundMyFitness Kim Chow Kim Chow 2 years ago good video my conclusion: take some if u r desperately needed cause boosters might disrupt the negative feedback loop Yoshinori Ohsumi: What is autophagy? A dynamic cellular recycling process 409,504 viewsDec 5, 2017 MoleCluesTV 8.35K subscribers Nobel laureate Yoshinori Ohsumi's lecture at the Molecular Frontiers Symposium at the Tokyo Institute of Technology, Japan, Oct 2017. The topic of the symposium was "Science for Tomorrow". Check our YouTube channel for more exciting science videos! For more information, visit www.molecularfrontiers.org 513 Comments rongmaw lin Add a comment... G Mo G Mo 3 years ago Finally a Nobel prize that went to someone who truly wants to help people bravo! 450 jeannette stuckelschwaiger jeannette stuckelschwaiger 1 year ago An outstanding scientist who didn't speaks english easily. He is highly educated and profoundly human. He has nothing to sell to anyone. May God bless him. 109 lovepeacebliss lovepeacebliss 3 years ago Hilarious how mostly everyone is complaining about his presentation. He won nobel prize for his discovery, not his speaking nor english skills. 1K Frank From Upstate NY Frank From Upstate NY 3 years ago Yoshinori sir,....thanks for your dedication,.....extremely hard work....discipline and sticktoitiveness. May God continue to bless you whilst on the "Path"... 139 Al C Al C 3 years ago The difference between knowing and understanding is huge. What a great quote by him. 82 Rick Stokes Rick Stokes 2 years ago A true researcher that started with a simple curiosity of those things not many people were interested in and now may be unlocking many fields of health and medicine with far reaching implications. Bravo Professor 44 Karen Almeria Karen Almeria 2 years ago Sir, for me your study of Autophagy is already a free medicine for many people and thus it became so popular and being quoted by many doctors as well. You are doing excellent and thank you so much we had a person like you in our generation. May God bless you more with longevity. 23 Thyag Sundaramoorthy Thyag Sundaramoorthy 3 years ago (edited) This groundbreaking, life-changing research on autophagy is being explained by the man himself, and all we have is random nobodys from the internet telling he needs to work on his speaking skills. 195 LIFE&LEMONS When life gives u lemons, make videos! LIFE&LEMONS When life gives u lemons, make videos! 1 year ago O am deeply honored to have even watched this video of a remarkable man and his discovery. I don't mind how he communicates. I'm just in awe! 16 pedroalvares cabral pedroalvares cabral 1 year ago Este prêmio Nobel para mim é o divisor de águas para a Saúde humana, parabéns para o Senhor Yoshinori Ohsumi!! 4 Purse & Foremost Purse & Foremost 3 years ago Sir, thank you for sharing your knowledge and expertise on this field. Congratulations for having won a Nobel prize. 26 ItsMeMeow ItsMeMeow 1 year ago Alhamdulilah...this month is Ramadhan,fasting month for Muslim than i found this video...watching this made me more to practice fasting properly...tq Profesor... 18 Edward H. Banksy Edward H. Banksy 3 years ago Just one complaint. 46 minutes of him is just too short. I want more! 115 Saraswathi S Saraswathi S 1 year ago Excellent research 👏👏👏👍hats off Sir ...Congratulations 💐you deserve many more awards...GOD bless you 🙏 7 Parbonee Dey Parbonee Dey 4 years ago His little contribution has brought a great change in our understanding. It was li'l difficult for me to understand his work previously, but now, since I did, I thank him for his contribution in biological science. I'm one of his fans now and will tend to figure out if it can further help humanity to fight diseases. 24 ZHIWEI QIN ZHIWEI QIN 4 years ago What an amazing discovery by this professor 14 Ryan Vacation Ryan Vacation 4 years ago Thank you for sharing with us your wisdom and knowledge 64 Hebasanch Hebasanch 2 years ago This is a great and useful discovery, thank you very much Yoshinori! Thanks a lot for sharing, MoleCluesTV! 10 Jayaprakash Rao Jayaprakash Rao 11 months ago Exceptional, dedicated Scientist...truly worked for good health of mankind...A great blessing for humanity....This world certainly requires the likes of you, Sir... 2 Rain Maker Rain Maker 2 months ago I appreciate your lecture, great information and discovery, yes, you deserved that Nobel prize!!! Jeff Harmed Jeff Harmed 2 months ago Wonderful research and a deserved Nobel prize. Anne Gallagher Anne Gallagher 3 years ago Thank you. Great lecture. I've been intermittent fasting only 2-weeks. Good to hear the science behind it. The graph 24, 36, ... 72 hours was informative. 16 G. Anna Smith G. Anna Smith 6 months ago (edited) Brilliant doctor 👏 Million thanks and massive congratulations Sir Ohsumi 👏 janette PT janette PT 8 months ago Just be thankful that he speaks english. Majority of Japanese highly educated, founders and CEOs do not speak english because they love their language. More than that, be thankful for his discovery because it might be key for life’s longevity for all of us. 2 Ahmet Kartal Ahmet Kartal 9 months ago Saygıdeğer şahsınız karşısında bütün insanlık size teşekkür borçludur. Sizin gibi insanlar çok değerli. Saygılarımımla.. Sander G Sander G 4 months ago Great contribution to humanity. Thanks for your valuable research and discovery. May God always guide you Anogoya Dagaati Anogoya Dagaati 2 years ago We should be ashamed of ourselves - plants and forests are the most resilient things in nature and from year to year they VISIBLY display autophagy - it took this beautiful Japanese man to point all humanity to the obvious. Survival depends, indeed requires long periods of deprivation. Everything* in nature is necessary! 22 Spritural Science Spritural Science 2 years ago This research will change the medical word...this research deserve minimum 10 Nobel prizes 13 António Manuel Vale António Manuel Vale 3 years ago So humble and so brilliant presentation. Greetings and special thanks from a portuguese admirer. 14 Benjamin Fowler Benjamin Fowler 3 years ago Congrats for such fantastic research and your contributions to medicine. Fasting has changed my life. 22 Amarat Mamu Amarat Mamu 1 year ago I can not believe that the camera person took about 5 min to realized that there was also a presentation with added information helpful to the presentation. We can hear him and being able to see the slides is a great complement to his talk. 2 a buddha a buddha 3 years ago Thanks. Nothing like getting your information straight from the horse's mouth... even if one must continue learning to fully appreciate what one has been offered. 8 King0fHArtford King0fHArtford 3 years ago i love this dude, incredible work. thank you mr Ohsumi. 16 A Ga A Ga 11 months ago Please accept our heartiest congratulations on your Nobel prize and our happiness at your winning it. My happiness and gratitude from National Taiwan University, Life Sciences, when I had the great chance of getting a seminar class lecture from you Sir. You spoke in detail for an hour about the discovery of the role of Atg molecules in cellular autophagy. Thank you, ba anni. 5 Orhideja Orhideja 2 years ago Браво, пост код православаца је Божји дар, тако се ми православци рециклирамо лечимо и приближавамо исчишћени Богу, жив и здрав био Yoshinori Bog ti dobrog dao...Amin... 7 Thomas Traynor Thomas Traynor 9 months ago I am learning even at age 68 yrs old. Autophagy! . A genius! 1 Der Hansl Der Hansl 4 months ago Just put on captions people, let this wonderful man speak about what we need to hear! Terry Thompson Terry Thompson 1 year ago Brilliant thought provoking research. 2 2710daisy 2710daisy 3 years ago Well deserved Nobel prize. 1 Noureddine Ali Noureddine Ali 3 years ago Respect to this man . 3 geeta nair geeta nair 4 months ago (edited) Arigato Yoshinori san....Your contibution to the world outstanding....Every nick and corner of the world trying to using this method.......once again thank you. lickan trollico lickan trollico 3 weeks ago (edited) Thank You, Sir...You solve a big part of the 'big puzzle'. Thank You. All the best. No Body No Body 3 years ago Love his message at the end. Very pleasant man, and pleasant presentation 19 William Rivas William Rivas 1 year ago Great presentation, thanks Doc! 2 Cinzia Mandati LB Cinzia Mandati LB 5 months ago Thank you Dr Oshinori! We need more like you! We also need to understand that we are destroying our planet in order to have all this food available to us, that at the end, we don’t need. 2 Faik Nasser Faik Nasser 4 months ago Great scientist with focus mind, hard working, humble he brings memories to me when I was young I worked under great Japanese scientist Professor Fusao Hirata at then pharmaceutical sciences Wayne state university I was working on Apoptosis Aaron Boster MD Aaron Boster MD 11 months ago outstanding presentation. thank you for sharing 1 Peter Lampropoulos Peter Lampropoulos 1 year ago He changed my life in a week and just getting better 10 MrRathel MrRathel 4 years ago Thanks for sharing with us this video. excellent presentation. 4 Soupy Sails Soupy Sails 3 years ago We are all watching a current day Einstein who’s research will heal and save many generations to come. 305 Vani The Voice Vani The Voice 3 years ago Sir , Namste !! Thank you for many showing light in the dark. Sir, thanks for proving technically which is following by many many cultures from many many centuries 6 Vivian House Vivian House 11 months ago Don't pay attention to his language, his knowledge and his Nobel achievement was very Amazing, anyway he is Japanese and can speak well English in his age is spectacular, APPRECIATED 3 ジェルリn ジェルリn 3 years ago A genius man. 9 Sachin Tamil Sachin Tamil 1 year ago This genius didn't waste his precious time in learning english instead he chose to learn science. 3 Uroš Nemec Uroš Nemec 2 years ago Last 6 monts I am eating at most time from 12 to 18 am. I am eating almost everithing healty foods than before, less shugar and much more litlle fishes in between lunch and dinner. I have been lost a lot of fat, and 7-8kg. I am feeling much much better than before, have more energy and mind is cleaner. Workout/training with my bodyweight is now 25-35min 4x in week. Before this 18/6 ritual i have been in fitnes for 4x ca 1,5hours weekly and almost never have so good results as now. Thank you sir to beeing, many times is so simple, but the industy/marketing told us that we should eat whole day 5-6x bla bla. 3 ColleenKaralee ColleenKaralee 5 months ago ​ That small beginning sparked massive further research into autophagy in human bodies. The NCBI and PubMed websites have search functions where one can find numerous articles into autophagy. Also Professor Valter Longo is doing applied research, starting with mice and rats and applying to humans, and he has come out with his own version of fasting diet to induce autophagy. Prof. Longo also featured in the BBC program, "Eat, Fast, and Live Longer". It is an eye opener. Empirical data for humans is gathering. 1 Kenny Starfighter Kenny Starfighter 2 years ago (edited) Thank you Yoshinori Ohsumi for proving fasting in science. It seems like we knew the benefits of fasting way back in time. Both tthe bible and the quran speaks about fasting. Our body loves to have rest from food intake it seems. 28 Makhan Singh Johal Makhan Singh Johal 6 months ago Amazing discovery 👍 Sir Lean -BEAUTY SUBLIMINALS - Sir Lean -BEAUTY SUBLIMINALS - 2 years ago really inspiring 2 Fahma sherat Thirukkovelluru Fahma sherat Thirukkovelluru 2 years ago Outstanding discovery 1 sonomasyrah sonomasyrah 3 years ago Great Talk. Love the information. 5 M0lecular Ep1phany M0lecular Ep1phany 3 years ago This is kinda like listening to Newton talk guys... this discovery is so profound. 5 Samir Goswami Samir Goswami 1 year ago This has been in practice from ages in our hindu culture...Proud to be a Hindu now 6 else better off get it naturally seems to me improving the salv