Evolution of ATP Synthase

Evolution of ATP Synthase 4,634 viewsMar 8, 2018 Jackson Wheat 7.76K subscribers An explanation of the evolution of ATP synthase. I made a slight mistake: when I say “V-ATP synthase,” I mean to say “V-ATPase” because the V-type ATPase doesn’t synthesize ATP. My bad. For Eric Batterson. All pictures are from Google. “Common Ancestry Pt. 6”: https://youtu.be/3ISSVpzV1QE “Cellular Respiration”: https://www.saylor.org/site/wp-conten... “Rotary DNA motors”: https://www.ncbi.nlm.nih.gov/pmc/arti... “ATP synthase, F1 complex, alpha subunit”: http://www.ebi.ac.uk/interpro/entry/I... “ATP Synthase”: http://www.ebi.ac.uk/interpro/potm/20... “Evolution of the F1-ATPase”: http://www.life.illinois.edu/crofts/b... “The ring-shaped hexameric helicases that function at DNA replication forks”: https://www.nature.com/articles/s4159... “The dTTPase mechanism of T7 DNA helicase resembles the binding change mechanism of the F1-ATPase”: https://www.ncbi.nlm.nih.gov/pmc/arti... “Gene duplication as a means for altering H’/ATP ratios during the evolution of F0,Fl ATPases and synthases”: http://onlinelibrary.wiley.com/doi/10... “The evolution of A-, F-, and V-type ATP synthases and ATPases: reversals in function and changes in the H+/ATP coupling ratio”: https://www.sciencedirect.com/science... “Inventing the dynamo machine: The evolution of the F-type and V-type ATPases”: https://www.researchgate.net/publicat... “ATP Synthase”: https://www.youtube.com/watch?v=CN2XO... “Electron transport in bacteria & archaea”: https://www.youtube.com/watch?v=uniFe... “ATP synthase: Structure and Function”: https://www.youtube.com/watch?v=b_cp8... 225 Comments rongmaw lin Add a comment... Rahul Rahul 4 years ago Thank you for making this video. Found your channel, and hence the video, from your comment about ATP synthase on The NonSequitur Show, and it looks like there's a lot of interesting stuff to explore here. And thanks for the references. :) Jackson Wheat James Downard James Downard 4 years ago Neat graphics (especially the moving ones). Given the fact that the ATPase system developed billions of years ago, so only the highly modified current systems are available to look at, its exciting how much of the evolutionary processes in their origin are worked out (and over just the last 20 years or so). 3 Jackson Wheat Jackson Wheat · Nesslig 20 Nesslig 20 4 years ago One minor correction. V-types are ATP*ases* not synthases because they break down ATP, but both ATPases and synthases are related structures nonetheless. 3 Jackson Wheat Jackson Wheat · Tony Reed Tony Reed 4 years ago This was awesome. I shouted you out in today's episode and this video is a perfect example of why I did. 2 Jackson Wheat Jackson Wheat · Dustin Furness Dustin Furness 4 years ago Thanks for telling this video exists. Nice work. Jackson Wheat Bill Willenbrock Bill Willenbrock 2 years ago How could you have a living cell if it has a helicase but not ATP synthase? Where would such a cell get it's energy? Some have theorized that the LUCA used fermentation but where would such a creature get the glucose to produce it's ATP if there are not photosynthetic organisms producing glucose? 4 sombodysdad sombodysdad 3 years ago For a gene duplication to have any affect it needs a new binding site. And according to the paper "Waiting for TWO Mutations" there isn't enough time for that. So forget about that and then mutating the new duplicate for some other function. 5 James Fletcher James Fletcher 3 weeks ago Helicase was in the first cell. Encoded in the first cell's DNA. zune153 zune153 4 years ago Great video JW. Guess who is going to accuse you of citation bluffing. 2 Jackson Wheat Jackson Wheat · Steven Baumann Steven Baumann 4 years ago Excellent bud! Jackson Wheat sombodysdad sombodysdad 1 year ago Anyone who thinks tat blind and mindless processes produced ATP synthase is already lost. Not only does the claim lack evidentiary support, it's untestable. It needs an external docking site to hold to two subunits together. An those docking sites don't have anything to do with the functionality of the subunits. Mike Bellamy Mike Bellamy 2 years ago Every stage in the presumed evolutionary sequence uses terms like "suggests" or "possibly" or "could have" etc.. with zero observational data. The variations in the ATP machinery used to support possible evolutionary explanations are observed in various EXTANT organisms ignoring the obvious fact they are COMPLETE and specifically suited to their different purposes consistent with being designed as such. What is truly amazing is the omission of the failed attempts to date to trace the assumed evolutionary ancestry of each of the 40 or so proteins involved. Instead the whole story begins with the assumed evolution of the major components being mega structures themselves of immense complexity, then imagining highly speculative mutations which we know by Shannon's Law destroy DNA information constructing whole new assemblies. The chance that these structures would evolve into a form precisely configured to fit together as they do to perform a new function is diabolical nonsense and not science. 1 Ad Brouwer Ad Brouwer 4 years ago At 1:03 I thought: "Ah, common designer." Startled myself, and switched back to science mode ☺ Jackson Wheat James Fletcher James Fletcher 3 weeks ago A living cell is more technologically advanced than anything humans have made. The structure of ATP synthase is still not fully understood. But remember evolution explains everything. Rayrard Rayrard 4 years ago Nice video... I was wondering why you chose the particular taxa you did in your intro? 1 Jackson Wheat Jackson Wheat · sombodysdad sombodysdad 3 years ago Thank you for PROVING that ATP synthase is irreducibly complex. 2 Jackson Wheat · daleportorford daleportorford 2 years ago Ancestral F1 and FO, could have, may have. Thank you for convincing me even more that this very complex mechanism was created by an intelligence. I call him God. So much speculation as usual. N factorial N factorial 1 year ago nice video man sombodysdad sombodysdad 3 years ago OK wait. Using gene duplication as a blind and mindless process shows desperation. You need to duplicate the gene and then build a proper binding site for it- proper meaning a binding site that allows for the expression of the gene at the right time and in the correct amounts. There isn't enough time in the universe 6 Rick Mulders Rick Mulders 3 years ago gene duplication and subsequent repurpousing is all over the tree of life. Prime example: Tequila gene in D. melanogaster, sombodysdad sombodysdad 3 years ago ATP synthase is irreducibly complex. This video in no way shows that ATP synthase evolved by means of blind and mindless processes. Once you rely on gene duplication you have left blind and mindless processes behind and are only relying on FAITH. 2 Jackson Wheat · sombodysdad sombodysdad 3 years ago And you expect people to believe that blind and mindless processes did it? Really? Not only do you need to produce the genes that code for the proteins, already outside the realm of blind and mindless processes, but you need to get their products expressed in the right quantities. Then you need to get them all in the same place and finally configured. However the two different subunits don't just attach. They are tethered together via an outside connection that has NOTHING to do with the functionality of either unit. It took planning to get that job done and get it done right. Jackson Wheat · Roedy Green Roedy Green 4 years ago The seems an overly complex molecule just to add a phosphorus. Jackson Wheat · Jungle Jargon Jungle Jargon 4 years ago Object credit givers are illogical and irrational, forever. Energy and biology go from order to disorder. Any rational and logical person would then ask where the ordering of energy and biology came from. You object credit givers are afraid to face the truth that energy/matter cannot make itself programmable or program itself. You ultimately believe that nature is magical rather than consider the obvious, that energy and biology were ordered by a Maker not made of or limited by the ordered energy, time and distance that He fabricated. Infinite regress of ordered and directed working things is always a higher order. That's the reason the Bible refers to the Maker of everything as the Most High. Eventually, you will arrive at the all knowing, all powerful Maker that is not limited or restricted by His fabrication of ordered energy, time and distance that He made. Our time has a beginning and an end. The Maker of time and distance has no end. Infinite regress will show you that, but what do you care about the truth? You are an object credit giver. You are very silly, forever. Your only hope is to stop giving all the glory to mindless unguided objects that can only ever be what they are forced to be and they only ever do what they are significantly sequenced, ordered and made and to do. 1 Jackson Wheat · Agrippa Agrippa 4 years ago Attacking the Holy Grail of Ronnie the Clown. Wonder if he will reply? If he does it will include the word "Liar" within the first sentence 6 Jackson Wheat Jackson Wheat · sombodysdad sombodysdad 3 years ago Mitochondria? Seriously? Only eukaryotes have mitochondria and you cannot get eukaryotes without ATP synthase. 1 Jackson Wheat · Atman Brahman Atman Brahman 6 months ago Just so stories 😂 Issou Amine Issou Amine 3 years ago what ?!!! good imagination especially the last study!!! but is it viable ? and it it is viable; is it plausible? and if it is plausible, did it really happen (some intermediary steps are needed)! waving hands over how a windmill (or a protonmill in this case) might have evolved from other buildings and facilities does not mean it evolved from them!!! after all they share walls and doors and windows...etc! but they don't share the fan and the turbines ...etc! 2 Jan Waska Jan Waska 1 year ago (edited) Where’s the beef? https://en.m.wikipedia.org/wiki/Where%27s_the_beef%3F Treedom Treedom 1 year ago Nick Lane ... watercool5 watercool5 1 year ago intro too long Mike Bellamy Mike Bellamy 2 years ago Not one critical thought here.. Does no one here have even a question.? Where did you people go to school? Start with the definition of "irreducibly complex" at 0:10 "unable to evolve step by step and remain functional at each stage".. correct. So now to "jump right in".. with just one question. ATP is an enzyme which transfers cellular energy.. at 0:55 then "ATP exists in all life on earth which indicates how old it is".. If it is a 'fact' that it exists in all life on earth.. doesn't that mean it is reasonable to conclude there can be no life without ATP? Then he follows at 1:00 with "meaning ATP and ATP Synthase were in the Last Universal Common Ancestor (LUCA) of all life". So the story of their supposed evolution which follows is not based on observation but on the ASSUMPTION that there was in the past a form of 'life' with neither or one without the other.! How is this science? On this point alone Mr Wheat has actually verified by observation the irreducible complexity of ATP Synthase (as he defined it)! They have always been there.. consistent with the biblical creation model. 1 Eric Batterson Eric Batterson 4 years ago The veiwer was me Jackson Wheat Jackson Wheat · blasater blasater 4 years ago (edited) Actually that is not a convincing answer at all. Show the steps...all of them...and show the genetic code changes, show the protein folding involved. I would like to see this setup in assay. That answer was just hand waving. Pointing to a motility motor as a precursor to a ATP production machine? No...wrong sequence. 2 Jackson Wheat · Jesse Bryant Jesse Bryant 4 years ago Sorry, begins with FAITH rather than SCIENCE. "All organisms contain ATP synthase, meaning that both ATP and ATP synthase were likely in the last universal common ancestor of all life." Is starting with a MAJOR assumption good science? (Science being things we can test, observe, and repeat.) Jackson Wheat Transport Physiology: 3D ATP Synthase (ATPase) 99,905 viewsMay 31, 2016 1.9K DISLIKE SHARE DOWNLOAD CLIP SAVE Lance Miller, PhD 20.9K subscribers This lesson explores how ATP is synthesized by the enzyme ATP synthase in the mitochondria. In particular, how NADH provides the necessary energy, how hydrogen ions are transported into the intermediate space, the transfer of electrons, and how the ATPase uses the hydrogen ion gradient to synthesize ATP. 68 Comments rongmaw lin Add a comment... Paulina Belvoncikova Paulina Belvoncikova 2 years ago That magic feeling when you can not understand ATPase after one semester of physiology, but finally get it after 5 min video :D Thanks 20 Hyumifu Hyumifu 1 year ago Oh my goodness, this is such a great video 😭 thank you so much for making it so simple 1 Geeta Rautela Geeta Rautela 3 years ago Your presentation is amazing sir I can understand very easily 2 Alex Israelyan Alex Israelyan 2 years ago Anyone using this as a primary source please note that the video has a number of errors. There is an error about the number of H+ ions pumped. It should be 4H+ from Complex I 4H+ from Complex III (through 2 semicycles that make up a full Q cycle) and 2H+ from Complex IV. Also by the way the Complex I does not at all transfer the 2 electrons to 2 Q in any way, Complex I has 1 binding site for 1 Q and transfers both electrons to 1 Q. And of course in Complex IV 2 electrons do NOT get transfered to 2 Oxygen molecules (the creator doesn’t seem to understand the difference between a molecule of O2 and 2 atoms of O), and of course besides that 4 electrons need to be transfered to an oxygen molecule so that it is reduced to 2 water molecules (the intermediate products wait in complex 2 until 2 more electrons arrive from another ETC cycle). There are 2 b subunits, not 1 as stated in the video. Anil Negi Anil Negi 2 years ago thankyou sir this was by far the best presentation i came through (a msc student who has an exam tommorow ) PRITAM SHAW PRITAM SHAW 2 years ago Best ever animation that is more than enough for this topic for depth knowledge! adyatv adyatv 2 months ago A quantum machine! For what is powering the whole thing is really just 'Protons' for a Hydrogen ion is really just a free proton! Great video, there is another video from HarvardX that shows the same with some really detailed protein graphics 👍 1 Suma Valli Suma Valli 1 month ago I thought it was soo tough to understand this processes before watching ur video .but now i am very clear about it vectorie:) vectorie:) 8 months ago Wonderful thank you so much প্ৰাণ প্ৰতিম প্ৰাণ প্ৰতিম 1 year ago Sir,please make video on biochemistry"s topics like oxidative phosphorylation,electron chain transport chain etch. Dulmini Senadheera Dulmini Senadheera 1 year ago Best explanation ever! Zetsuke4 Zetsuke4 2 years ago Wow this is incredible! 1 Nazish Amin Nazish Amin 3 years ago Thanks a lot sir ,it helped me a lot Basic Science Basic Science 1 year ago Thanks sir best animation. Very helpful video Dinesh Gaikwad Dinesh Gaikwad 1 year ago Amazing to see this amazing machinery is present in some of the most douche people on Earth! 2 Bozorgmanesh Robert Sohrabi Bozorgmanesh Robert Sohrabi 2 years ago I am a beginner learning that hydrogen is the first valuable element in the Periodic Table. Priyasmita Das Priyasmita Das 3 years ago Just the right video that I needed to watch!!! R Harr R Harr 4 years ago 3 free phosphates disliked this video. Thanks for making a good video. 17 Jere616 Jere616 3 years ago Very succinctly and clearly explained. Thank you. Not seen too many this well stated. (BTW, to improve the narration, "comprised" is not followed by "of." It would be, for example, "It comprises 10 C subunits." ) Benjamin Franklin Joseph Benjamin Franklin Joseph 4 years ago Great. Thank you. Peter S Peter S 3 years ago If the mitochondria was once a free cell colonized by the more complex cell, was the matrix once a free cell colonized by the mitochondria? Alisa Yu Alisa Yu 2 years ago Almost cried...thx for making a good video 8 Saloni Pardeshi Saloni Pardeshi 4 years ago Every nice explanation..thank u. 1 akumarisu akumarisu 3 years ago Is it 2 H+ at Complex III and 4 H+ at Complex IV or other way around? I keep seeing 4 H+ at Complex III and 2 H+ at IV on other sources for some reason. 7 Mohammad Abubakar Mohammad Abubakar 2 years ago Wow its awesome sir 2fast2block 2fast2block 2 years ago Another of God's beautiful designs. Thanks. 11 Jose David Forero Jose David Forero 4 years ago Please a question. This machinery is the same in all species? Thank you. obi christiana obi christiana 1 month ago Thanks I understand properly now Alex Nalli Alex Nalli 1 year ago This video highlights the importance of playing with Lego or Playmobil as a child if you want to be a scientist. Chris Capablanca Chris Capablanca 2 years ago And now we also know what happens to this process when we eat the wrong poisonous foods, in the middle of this video the breakdown of the proton gradient. This whole video is important. We seem to now know the cause of most all modern disease. https://www.youtube.com/watch?v=pHnPinYI2Yc 2 shabnam sharma shabnam sharma 3 years ago How many ATP molecules formed in this process. Because total 10 H+ ions are transferred and 3H+ formed 1 ATP molecules....... Tom Bal Tom Bal 2 years ago I have a question, have this "assembly machine" been phisically seen, like from a microscope, or its appearence is just theoric? Like if the scientists hypotize it should be built this way, but they never been able to materially see it? Luke Schneider Luke Schneider 3 years ago I subscribed before even watching the VIDEO!! 😜 GREAT!! 1 Lost Boi Lost Boi 2 years ago Best video on this topic 1 Sindhu M Sindhu M 1 year ago Thank you very much alireza nourozi alireza nourozi 1 year ago awesome 2 satyam agrahari satyam agrahari 3 years ago At 2:15 you said that 2 electrons bind to 2 oxygen MOLECULES but there is only 1 oxygen molecule shown. 2 oxygen molecule have 4 oxygen atom it should combine to 8 H + ions that is confusing 3 Hosoi Archives Hosoi Archives 4 months ago Where do the hydrogen ions come from Halima Noor Halima Noor 4 years ago Thanks alot .. Hosoi Archives Hosoi Archives 4 months ago Where does the nadh come from Evan Hodge Evan Hodge 1 year ago (edited) Is reality this neat though? Hard to believe. Life is messy and stinks. This concept looks like some machine. Sambedana Sambedana 2 years ago But one complete rotation produces 3 atp...according to ur explanation it shows only one... How??? josiah harder josiah harder 2 years ago Why have I heard from several other videos that ATP synthase is powered by proton flow but this video says hydrogen is used? รςคlคຟคງ รςคlคຟคງ 2 years ago Atoms: I just want to be stable Biology: Gooood, Gooooooood Mr Squiggles Mr Squiggles 2 years ago what happened to complex 2? 2 Hosoi Archives Hosoi Archives 4 months ago Why do mitochondria make water? lety rincon lety rincon 2 years ago Dame money AlphaOne2009 AlphaOne2009 2 years ago What? ·