Monday, May 28, 2018

Bacteria and the body

Bacteria and the body

Welcome to the Bacteria-on-your-skin learning project.
This article over at Physorg.com noted that there are many more bacteria growing on the skin of healthy individuals than we previously knew. Included in the article are distributions of different bacteria on people's skin.
Estimating values from the article, it appears, on average: 50% of bacteria are "actinobacteria" then with a wide varibability between subjects: 25% of bacteria are Firmicutes and another 25% are proteobacteria. On one subject, 24% of his or her bacteria was "other".
What are these different types of bacteria that probably grow on skin then?

Firmicutes[edit]

According to Wikipedia:Firmicutes:
The Firmicutes are a division of bacteria, most of which have Gram-positive cell wall structure. A few, the Mollicutes or mycoplasmas, lack cell walls altogether and so do not respond to Gram staining, but still lack the second membrane found in other Gram-negative forms. Others, such as Megasphaera, Pectinatus, Selenomonas, and Zymoph Actinobacteria. They have round cells, called cocci (singular coccus), or rod-shaped forms.
Many Firmicutes produce endospores, which are resistant to desiccation and can survive extreme conditions. They are found in various environments, and some notable pathogens. Those in one family, the heliobacteria, produce energy through photosynthesis.
The group is typically divided into the Clostridia, which are anaerobic, the Bacilli, which are obligate or facultative aerobes, and the Mollicutes. On molecular trees the first two groups show up as paraphyletic or polyphyletic, as do their main genera, Clostridium and Bacillus. It is likely these groups will undergo revision.
Firmicutes play an important role in beer, wine, and cider spoilage.
Researcher Jeffrey Gordon and his colleagues found that obese humans and mice had a lower percentage of a family of bacteria called Bacteroidetes and more Firmicutes. But they are not sure if Firmicutes cause obesity or if people who are obese grow more of that type of bacteria.
While there are currently more than 274 genera within the Firmicutes phylum, Notable genera of Firmicutes include... Wow. Not having advanced training in biology, that is way over my head. Let's break it down...

Gram-positive bacteria[edit]

Gram-positive bacteria are classified as bacteria that retain a crystal violet dye during the Gram stain process. Gram-positive bacteria will appear blue or violet under a microscope, whereas Gram-negative bacteria will appear red or pink. The difference in classification is largely based on a difference in the bacteria's cell wall structure. Okay, so what?
In the original bacterial phyla, the Gram-positive forms made up the phylum Firmicutes, the name now used for the largest group. It includes many well-known genera such as Bacillus, Listeria, Staphylococcus, Streptococcus, Enterococcus, and Clostridium. It has also been expanded to include the Mollicutes, bacteria like Mycoplasma that lack cell walls and so cannot be stained by Gram, but are derived from such forms.
The actinobacteria are another major group of Gram-positive bacteria; they and the Firmicutes are referred to as the high and low G+C groups based on the guanine and cytosine content of their DNA. If the second membrane is a derived condition, the two may have been basal among the bacteria; otherwise they are probably a relatively recent monophyletic group. They have been considered as possible ancestors for the archaeans and eukaryotes, both because they are unusual in lacking the second membrane and because of various biochemical similarities such as the presence of sterols.
The Deinococcus-Thermus bacteria also have Gram-positive stains, although they are structurally similar to Gram-negative bacteria.
Both Gram-positive and Gram-negative bacteria may have a membrane called an S-layer. In Gram-negative bacteria, the S-layer is directly attached to the outer membrane. In Gram-positive bacteria, the S-layer is attached to the peptidoglycan layer. Unique to Gram-positive bacteria is the presence of teichoic acids in the cell wall. Some particular teichoic acids, lipoteichoic acids, have a lipid component and can assist in anchoring peptidoglycan, as the lipid component is embedded in the membrane.
That still doesn't tell me a whole lot of useful information. That makes me wonder if learning about Firmicutes could give me any knowledge that can enhance my health, happiness or wellbeing.

Endospore[edit]

An endospore is a dormant, tough, non-reproductive structure produced by a small number of bacteria from the Firmicute family. The primary function of most endospores is to ensure the survival of a bacterium through periods of environmental stress. They are therefore resistant to ultraviolet and gamma radiation, desiccation, lysozyme, temperature, starvation, and chemical disinfectants. Endospores are commonly found in soil and water, where they may survive for long periods of time. Some bacteria produce exospores or cysts instead.
So this is what about 25% of the bacteria on my skin produce. I am morbidly fascinated.

Actinobacteria[edit]

50% of the bacteria on my skin might be actinobacteria... From wikipedia:Actinobacteria
The Actinobacteria or Actinomycetes are a group of Gram-positive bacteria. Most are found in the soil, and they include some of the most common soil life, playing an important role in decomposition of organic materials, such as cellulose and chitin. This replenishes the supply of nutrients in the soil and is an important part of humus formation. Other Actinobacteria inhabit plants and animals, including a few pathogens, such as Mycobacterium.
Some Actinobacteria form branching filaments, which somewhat resemble the mycelia of the unrelated fungi, among which they were originally classified under the older name Actinomycetes. Most members are aerobic, but a few, such as Actinomyces israelii, can grow under anaerobic conditions. Unlike the Firmicutes, the other main group of Gram-positive bacteria, they have DNA with a high guanine and cytosine concentration.
Interesting things to note: some produce spores, and some decompose organic material.

Proteobacteria[edit]

Perhaps 25% of the bacteria on our skin may be proteobacteria.
Wikipedia:Proteobacteria says: The Proteobacteria are a major group of bacteria. They include a wide variety of pathogens, such as Escherichia, Salmonella, Vibrio, Helicobacter, and many other notable genera. Others are free-living, and include many of the bacteria responsible for nitrogen fixation. The group is defined primarily in terms of ribosomal RNA (rRNA) sequences, and is named for the Greek god Proteus (also the name of a bacterial genus within the Proteobacteria), who could change his shape, because of the great diversity of forms found in it.
All Proteobacteria are Gram-negative, with an outer membrane mainly composed of lipopolysaccharides. Many move about using flagella, but some are non-motile or rely on bacterial gliding. The last include the myxobacteria, a unique group of bacteria that can aggregate to form multicellular fruiting bodies. There is also a wide variety in the types of metabolism. Most members are facultatively or obligately anaerobic and heterotrophic, but there are numerous exceptions. A variety of genera, which are not closely related, convert energy from light through photosynthesis. These are called purple bacteria, referring to their mostly reddish pigmentation.

Gram-negative[edit]

Gram-negative bacteria are those that do not retain crystal violet dye in the Gram staining protocol. Gram-positive bacteria will retain the dark blue dye after an alcohol wash, whereas Gram-negative bacteria do not. In a Gram stain test, a counterstain is added after the crystal violet, which colors all Gram-negative bacteria a red or pink color. The test itself is useful in classifying two distinctly different types of bacteria based on structural differences in their cell walls.
Many species of Gram-negative bacteria are pathogenic, meaning they can cause disease in a host organism. This pathogenic capability is usually associated with certain components of Gram-negative cell walls, in particular the lipopolysaccharide (also known as LPS or endotoxin) layer.

Interesting things to note[edit]

Many proteobacteria are pathogenic, gram negative, and RNA.

Conclusions and what I have learned[edit]

It is interesting to know that the various bacteria on my body have different classifications. I assumed this was the case considering my previous schooling but had no idea what they were or the different characteristics of each.
It would be interesting to know what the hygenic habits are of the participants of this study. When was their last shower? How clean do they keep their rooms. What sort of environments do they frequent. It seems using an ecological systems approach more data/insights could be gleamed about the methods of creating the most healthy environments. While all these individuals are "healthy" perhaps some of them have a sore throat more often than others or perhaps some have colds more often than the others.
This learning project is an experiment in Wikiversity for my own satisfaction and learning. Please, if you are interested, add to it, edit it, improve it etc.

Stomach Bug Appears to Protect Kids from Asthma

Article ID: 542505
Released: 10-Jul-2008 9:00 AM EDT
Source Newsroom: NYU Langone Health
  • H. pylori
Newswise — A long-time microbial inhabitant of the human stomach may protect children from developing asthma, according to a new study among more than 7,000 subjects led by NYU Langone Medical Center researchers. Helicobacter pylori, a bacterium that has co-existed with humans for at least 50,000 years, may lead to peptic ulcers and stomach cancer. Yet, kids between the ages of 3 and 13 are nearly 59 percent less likely to have asthma if they carry the bug, the researchers report. The study appears in the July 15, 2008, online issue of The Journal of Infectious Diseases.
"Our findings suggest that absence of H. pylori may be one explanation for the increased risk of childhood asthma," says Yu Chen, Ph.D., assistant professor of epidemiology at New York University School of Medicine and a co-author of the study. "Among teens and children ages 3 to 19 years, carriers of H. pylori were 25 percent less likely to have asthma."
The impact was even more potent among children ages 3 to 13: they were 59 percent less likely to have asthma if they carried the bacterium, the researchers report. H. pylori carriers in teens and children were also 40 percent less likely to have hay fever and associated allergies such as eczema or rash.
These results, which follow on from similar findings in adults published by the same authors last year, are based on an analysis of data gathered from 7,412 participants in the fourth National Health and Nutrition Survey (NHANES IV) conducted from 1999 to 2000 by the National Center for Health Statistics. Dr. Chen collaborated on the survey with Martin J. Blaser, M.D., the Frederick H. King Professor of Internal Medicine, chair of the department of medicine, and professor of microbiology at NYU Langone Medical Center. Dr. Blaser has studied H. pylori for more than two decades.
Asthma has been rising steadily for the past half-century. Meanwhile H. pylori, once nearly universal in humans, has been slowly disappearing from developed countries over the past century due to increased antibiotic use, which kills off the bacteria, and cleaner water and homes, explains Dr. Blaser. Data from NHANES IV showed that only 5.4 percent of children born in the 1990s were positive for H. pylori, and that 11.3 percent of the participants under 10 had received an antibiotic in the month prior to the survey.
The rise in asthma over the past decades, Dr. Blaser says, could stem from the fact that a stomach harboring H. pylori has a different immunological status from one lacking the bug. When H. pylori is present, the stomach is lined with immune cells called regulatory T cells that control the body's response to invaders. Without these cells, a child can be more sensitive to allergens.
"Our hypothesis is that if you have Helicobacter you have a greater population of regulatory T-cells that are setting a higher threshold for sensitization," Dr. Blaser explains. "For example, if a child doesn't have Helicobacter and has contact with two or three cockroaches, he may get sensitized to them. But if Helicobacter is directing the immune response, then even if a child comes into contact with many cockroaches he may not get sensitized because his immune system is more tolerant."
In other words, the presence of the bacteria in the stomach may influence how a child's immune system develops: if a child does not encounter Helicobacter early on, the immune system may not learn how to regulate a response to allergens. Therefore, the child may be more likely to mount the kinds of inflammatory responses that trigger asthma.
"There's a growing body of data that says that early life use of antibiotics increases risk of asthma, and parents and doctors are using antibiotics like water," Dr. Blaser says. "The reality is that Helicobacter is disappearing extremely rapidly. In the NHANES IV study, less than six percent of U.S. children had Helicobacter, and probably two generations ago it was 70 percent. So, this is a huge change in human micro-ecology. The disappearance of an organism that's been in the stomach forever and is dominant is likely to have consequences. The consequences may be both good—less likelihood of gastric cancer and ulcers later in life—and bad: more asthma early in life."
ABOUT NYU LANGONE MEDICAL CENTER
One of the world's premier academic medical institutions for more than 167 years, NYU Langone Medical Center continues to be a leader in patient care, physician education and scientific research. NYU Medical Center is internationally renowned for excellence in areas such as cardiovascular disease, pediatrics, skin care, neurosurgery, urology, cancer care, rehabilitation, plastic surgery, minimally invasive surgery, transplant surgery, infertility, women's health and day surgery.

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