Exploration of the Powerhouse: Working Towards a Deeper Understanding of...

Exploration of the Powerhouse: Working Towards a Deeper Understanding of Mitochondria Function Hope College 4.95K subscribers Subscribe Like Share Download Clip Save 462 views Apr 21, 2022 Dr. Kristin Dittenhafer-Reed (Chemistry) Exploration of the Powerhouse: Working Towards a Deeper Understanding of Mitochondria Function Mitochondria, often referred to as the powerhouse of the cell, are essential for cellular energy production and proper cell function. My interest in understanding mitochondrial function began as an undergraduate and evolved through my research career. I will describe ongoing projects in my lab focused on understanding the molecular mechanisms that act within the mitochondria to control energy production. I'll also discuss my own understanding of my work as vocational Christian scholarship and discuss the important role that mentoring students plays in this work. * * To sign up for an in-person tour, visit https://hope.edu/admissions/visit/ To learn more about our academics, check out https://hope.edu/academics/ Follow Hope College on Instagram / hopecollege Key moments View all Transcript Follow along using the transcript. Show transcript Transcript Search in video 0:10 perfect thank you andy and thanks to both andy and lindsay for their leadership of the continuum 0:16 scholars program this summer as well as all of the other faculty who played a part in this as the 0:22 continuum scholars it was great we had really fruitful conversations and time 0:27 to reflect on things that i hadn't before during my time at hope at least not with others 0:32 so i will be talking a little bit today about my work with the mitochondria i love this image that's on the slide 0:39 right here all the green lines there are mitochondria within a cell and they kind of form this like energetic life force 0:45 that we'll talk about a little bit more as we go on so today what i hope to talk about uh 0:52 what are the mitochondria and why am i excited about studying mitochondrial function so i'll give you a little taste 0:59 of that then i'll tell a short snippet of ongoing research in my lab that's 1:05 focused on the regulation of mitochondrial dna transcription and then finally 1:10 end with a discussion of my faith and my scholarship so first what are mitochondria and so as 1:18 we've already covered mitochondria are the powerhouse of the cell and so again from kind of any introductory biology 1:25 class even as far back as probably middle school we learn about the different compartments of a cell and i 1:31 can remember like putting cell models together and putting little pieces in parts with styrofoam or something 1:36 and i always kind of love the mitochondria we think of it as this little bean shaped thing that you see on that mug 1:42 remember this mug i'm going to talk about this mug later in the talk but we'll go from there 1:48 so what are mitochondria we can also answer this question star wars has a great uh answer to what 1:54 our mitochondria so i'm going to show a quick clip here that will help us to understand what mitochondria are 2:03 master what are midichlorians midichlorians are a microscopic life 2:09 form that resides within all living cells they live inside me inside yourselves 2:15 yes and we are symbians with them symbionts life forms living together for 2:22 mutual advantage without the midichlorians life could not exist and we would have no knowledge of 2:28 the force they continually speak to us telling us the will of the force 2:35 the force the force is what gives the jedi is power 2:40 it's an energy field created by all living things it surrounds us midi chlorians are a 2:46 microscopic but this is going to be a problem if this keeps happening so let's see here 2:55 is that better okay maybe it was just twisted a little bit okay so the quote there is midi 3:01 chlorians are a microscopic life form that reside inside of our living cells without the midichlorians life could not 3:07 exist and they also explain the force okay so that's all i have to say today that's why i study mitochondria um 3:14 no i'm joking right so this is a funny little clip that i'm not the only mitochondrial biochemist or biologist 3:19 who likes to show this thing it's kind of a joke but there's a lot of things that are kind of true in here right like 3:24 life couldn't exist without mitochondria they are sort of a microscopic life form that reside inside our cells 3:31 through this process of symbiosis and so i'm going to hit on all of those things as we go forward um but let's dig in a 3:38 little bit deeper what are the mitochondria so the powerhouse what does that mean that mitochondria are the 3:44 powerhouse of the cell what mitochondria are required to generate most of the this molecule called adenosine 3:50 triphosphate or atp we'll ignore the chemical structure and just focus kind of on these three circles which are the 3:56 phosphates of that molecule and we can think of atp as running around the cell as like a charged 4:02 battery and it helps reactions occur that normally energetically wouldn't be 4:08 favorable so the hydrolysis of adp atp or the breakdown to adp now we have two 4:15 phosphates here is often coupled with a large number of different metabolic reactions that again could not occur 4:22 unless we use atp with it now where did the mitochondria come back in will the mitochondria come in in that 4:29 they regenerate this atp so they take the dead battery adp that's not really 4:34 useful for anything and they synthesize a ton of atp and so without this a number of 4:42 metabolic reactions couldn't happen life as we know it in our cells couldn't occur because we wouldn't have atp 4:49 and we need the mitochondria to generate this energy currency which is why they're known as the powerhouse of the 4:55 cell just some fun facts here about 110 pounds of atp are converted to 5:02 adp daily this is a ton right that doesn't mean 110 pound person is all atp 5:08 what's happening here is that each atp equivalent is recycled more than 500 5:14 times each day okay so the mitochondria have to be there to recycle adp to atp 5:21 and this process is occurring rapidly and all of the time and so that's why 5:26 the mitochondria get uh their name as the powerhouse of itself 5:33 there's some other fun facts about the mitochondria if i haven't sold you on them yet with star wars in the powerhouse 5:38 there are 10 million billion mitochondria in a human that is approximately 5:45 10 of our body weight mitochondrial proteins i'm a protein biochemist i get excited about studying 5:52 the machines of our cells the proteins within our cells that do kind of all of this work that we 5:58 need them to do account for about seven to ten percent of the entire human proteome 6:04 and then finally uh the mitochondria are central to metabolism of all sorts of dietary fuels so the mitochondria don't 6:12 just make atp they do a whole ton of other things beyond even just these 6:18 beyond just metabolism and when i think about my interest in the mitochondria and how i got to studying the 6:24 mitochondria i think it begins with metabolism and this interest in metabolism i remember being a kid and 6:30 and really being interested in maps and puzzles and logic and how does this all work together and when we think about 6:37 metabolism so this is a metabolic map but it's not intended for you to be able to read the pieces but just that you can 6:43 see right all of these different chemical conversions so each kind of line here is some chemical reaction 6:49 that's occurring within our cell that's involved in metabolism and i think it's beautiful that all of 6:55 these things happen in our cells it's regulated it's complex and trying 7:00 to understand how all of this works together is something that really interests me and the mitochondria which 7:06 if we look just kind of at this dark gray box all of these metabolic reactions are occurring within the mitochondria they're really central hubs 7:13 to cellular metabolism additionally another reason why i'm 7:20 excited about the mitochondria is this idea about their importance in human health and disease i think i've heard a 7:27 number of colleagues share a similar story that they entered into undergrad thinking that they were going to go into 7:32 medical school they were interested in science and they wanted to help people and med school was the way that you would do that and i was there i was 7:39 there probably until the end of my junior year i was pretty convinced i was going to med school 7:44 and some things happened i said oh no that's not what i want to do and so i i transitioned into realizing 7:52 you could use science as a researcher to also impact human health and understand human 7:58 disease so again i used to say to students tell me a disease and i'll tell you how the 8:03 mitochondria is implicated now that's hubris right it's overstatement but there's a little bit of truth that as 8:10 mitochondria began to fail we see these types of things in aging if we think of them as engines and as your engine 8:16 becomes less efficient right you release heat that's bad for a car similarly that's bad for a person as we get less 8:23 efficient mitochondrial function so mitochondrial dysfunction has been implicated in diseases like cancer 8:29 neurodegenerative disorders specific genetic kind of mutations within the 8:34 mitochondrial genome that i'll talk about that then cause disease and so 8:41 metabolism gets me excited about the mitochondria and their roles in disease also gets me excited about the 8:46 mitochondria so the goal of my lab at hope is to understand basic biochemistry of 8:52 mitochondrial function in order to enable a deeper understanding of mitochondrial dysfunction that occurs in 8:58 human disease so we don't come at it from a disease perspective i don't say our lab studies cancer i say our lab 9:04 wants to understand better how the mitochondria work in hopes that someone else might be able to use that 9:10 information in the context of a human disease and everything that we do is focused on 9:17 the mitochondrial genome or mitochondrial dna which is shown in this picture so again we see our 9:23 little bean of a mitochondria and within each mitochondria there are number of copies of mitochondrial dna 9:30 and where you might be most familiar with mitochondrial dna is through lineage tracing because it's thought to be maternally inherited and so some 9:37 people can you so you can trace mitochondrial dna throughout kind of um 9:44 maternal lineage lineages perhaps even to some mitochondrial eve shall we say 9:50 but i'm excited about mitochondrial dna for for some other reasons that i'll tell you about so mitochondrial dna again the small 9:57 circular genome recall we have genetic information now in two places in the mitochondria as well as in the nucleus 10:03 right where we think of the bulk of our dna before i get too far i know that this 10:09 audience we have people from all different places so i want to go through some words that i'm going to use and 10:14 make sure we all have the same kind of background so a central dogma of biology 10:20 we start with dna so either mitochondrial dna or dna in our nucleus we can think of this dna as like the 10:27 blueprint to an entire house through the process of transcription that's a word i'll use a lot in this 10:33 talk because we study transcription through the process of transcription we make what are called messenger rnas or 10:39 mrnas so that's like saying hey we want to build the door of the house 10:44 we don't need all of the instructions in the dna we just need the instructions for the door only so we pull the 10:50 instructions from the door only and make a message of that through the process of translation we go 10:57 from mrna and we synthesize our proteins so much like we could take the blueprint 11:02 for the door and then we could build the door okay so that's kind of these words that i'll use and talk about 11:11 so what do we study in the lab what what are we thinking about so we start up here with a mammalian cell 11:17 nucleus and mitochondria of course there are other bits and pieces of that cell but we're focused right now on just the 11:23 nucleus and the mitochondria and your cell could be likened to a city where all these different parts have to work 11:29 together in order for appropriate function to occur 11:34 let's focus first on the mitochondrial genome the mitochondrial genome codes just for 13 proteins and those 13 11:41 proteins are required for cellular energy production much like a power plant would be required for energy 11:48 production and distribution in a city now if we compare that to the nuclear 11:54 genome the nuclear genome carries the genetic information for over 20 000 proteins so this is orders of magnitude 12:00 greater when we compare the mitochondrial genome to the nuclear genome and this directs cell function 12:07 right much like city hall directs the city function city hall might be governed by a city 12:12 council the nuclear genome is controlled by transcription factors among many other things 12:19 now what i find most interesting when we think about the nuclear genome in the mitochondrial genome 12:25 is that there are a large number of proteins 1500 to 2000 proteins of which 12:31 the genetic information is carried within the nucleus but those proteins must be transcribed and translated into 12:37 proteins and then shipped into the mitochondria in order for them to to carry out their function 12:44 some of these proteins include transcription factors for mitochondrial dna so these things that control 12:51 the transcription dna to mrna also the protein machinery that's 12:56 required for transcription of mitochondrial dna so without the nucleus the mitochondria 13:02 cannot transcribe its own mitochondrial dna it's useless the mitochondria dna is 13:07 useless inside of the mitochondria unless the nucleus is there to help it and i think that that's really 13:13 interesting and then finally we talked about the powerhouse and the synthesis of atp 13:19 so there's 90 protein puzzle pieces that are required in order to generate atp directly 13:25 13 of those are encoded by the mitochondrial genome the remainder are encoded by the nuclear 13:31 genome so the mitochondria on its own cannot even make atp it relies on other 13:37 protein subunits that are that the information is carried in the nuclear dna in order for that to work 13:44 so this sets up a situation where these two genomes must communicate with each other in order to match kind of 13:50 energetic needs the cell says hey i need more atp the mitochondria can't go off and do its own thing without kind of the 13:57 nucleus being there to potentially help as well so the big questions in my lab 14:03 are how do the mitochondria nucleus communicate to connor to coordinate their transcriptional response to 14:09 changes in cellular energy demands so again if this idea we need more energy we need more atp kind of long term well 14:17 we need to boost the transcriptional machinery potentially which requires a nucleus we might need to boost 14:24 the number of components of the energy production machinery again relying on the nucleus how are these signals 14:30 relayed between the two compartments of the cell that's the big picture question that kind of drives my research and my 14:37 interest the second question is how is mitochondrial dna transcription 14:43 regulated in human cells and the third question which kind of is 14:48 tries to address questions one and two are that a number of proteins about 60 are known to associate with 14:54 mitochondrial dna but their roles in transcription and transcriptional regulation are unknown so my hope during 15:01 my career is to take a stab maybe not at all 60 but to start 15:06 plugging away at these proteins known to associate with mitochondrial dna and say are they doing anything important 15:13 in both protecting potentially the genome or regulating transcription of the genome 15:21 so what's our hypothesis what are we studying in the lab directly so our hypothesis is that things called protein 15:28 post-translational modifications regulate mitochondrial dna transcription so i presented this 15:34 this just to you a few slides ago the central dogma there's another layer of complexity on top of that central dogma 15:40 and those are these things called post-translational modifications where we can decorate the amino acids the 15:47 chemicals that are linked together in order to form proteins we can decorate them with different chemical 15:52 modifications and that changes the function of that protein so for example we could add a mail slot 15:58 to the door or we could put windows on the door we could take the doorknob away right so we've changed the function by 16:04 changing the chemical makeup of that protein so what do these looks like look like i 16:10 got to show a few structures here so one of the post-translational modifications we think about is phosphorylation 16:16 so phosphorylation occurs on hydroxyl groups or alcohol groups on three 16:22 different amino acids within our proteins and we can see chemically right that this is different so we've gone 16:28 from neutral to negative charge bigger kind of molecule on there so we can envision how sticking that in 16:35 potentially to a protein that binds to mitochondrial dna we've now stuck this negative charge in there and made it a 16:41 little bigger that could disrupt some interaction or cause an impact importantly these are reversible which 16:47 is kind of cool right so a protein puts this modification on a protein can take it off so it can act like a switch an on 16:54 off switch for protein function another modification that we think about is this modification called acetylation 17:02 and so again we're focused on the change that occurs to this nh3 group it's often positively 17:09 charged we've lost this pot of positive charge and again kind of changed the structure of that amino acid 17:18 it turns out that mitochondrial proteins have a ton of amino acids that are modified by these different types of 17:24 chemical groups so about 2500 acetylated lysine so a lysine is the amino acid and 17:30 i'll use the word site to refer to these modified places on proteins a 17:36 thousand phosphorylation sites and they're involved in all sorts of different processes within the 17:41 mitochondria and so my work back in grad school kind of started with trying to explore these different modification 17:47 sites that really kind of launched me into this interest in mitochondrial dna because i saw lots of modifications on 17:54 proteins that were involved in mitochondrial dna transcription and maintenance 18:01 further about 5 maybe less have a characterized biochemical function and so while we're 18:07 really interested in the lab and understanding mitochondrial dna transcription this is also helping to understand kind 18:14 of the bigger picture of why are these modifications within the mito mitochondria 18:22 so i'll tell you about three of our favorite proteins that we're focused on so this is a protein crystal structure 18:28 i think they're quite beautiful i like to look at them another reason why i like protein biochemistry uh what we're 18:34 looking at here in the orange and the blue and the green that's dna and these three proteins pull rmt which 18:41 is the polymerase it's the enzyme that actually synthesizes the mrna 18:47 in yellow here we see a transcription factor this transcription factor is important to get the polymerase at the 18:54 right spot on the mitochondrial dna and then the red protein here is tfb2m 18:59 or transcription factor b2 and it's super important and again kind of this appropriate alignment as well as 19:06 opening the dna up that's what promoter melting means we have to open the dna up in order for the polymerase to get in 19:12 there and start doing its job so a lot of our work is focused on 19:18 tfb2m although each of these proteins have documented 19:23 modification sites and this is either from literature from some work that we've done in the lab with the mass 19:29 spectrometer that we have that can kind of identify where on the protein these modifications exist 19:37 if you look closely you see kind of these space filling balls on tfb2m those are three modification sites that we've 19:43 studied and sought to understand their function i'm going to change colors here a little 19:48 bit i like this image a little bit better in order to see these sites but s197 that means serine 197 so one of 19:56 those amino acids in that protein which is known to be phosphorylated and then we have threonine 313 and 20:03 threonine 184 so three amino acids all known to be phosphorylated nobody knew 20:08 what they were doing if they played any important role in protein function 20:14 this s197 we looked at so i often have students look at these pictures and i say make a hypothesis 20:20 tell me what you think could be going on if the chemical structure of that amino acid changes 20:26 and so this really great kind of thing to look at and kind of investigative work we can do before even doing any wet 20:32 lab experiments in the lab so how do we study this 20:38 what we do is we use e coli we trick e coli into becoming little protein synthesis machines so much like an 20:46 organic chemist can synthesize an organic molecule and purify it we can have e coli synthesize a big organic 20:53 molecule a big protein and we can purify it away that's great but we have to somehow 21:00 mimic this phosphorylated group so here's just one example here we see the amino acid serine with it in its 21:06 phosphorylated form unfortunately we can't easily just make a protein 21:12 in the lab that's phosphorylated at a single site so we can't do that we have to mimic that phosphorylation in some 21:19 other way so we can mimic it by changing the amino acid at the position to either 21:25 an aspartate or a glutamic acid and you can see now that those 21:30 kind of look a little more similar than just an oh group okay and so we use 21:36 these what we call modification mimics to purify libraries of proteins that are 21:44 modified and then we go ahead and we study those in a couple of different ways so once we purify our proteins of 21:50 interest we can look at transcription inside of a test tube so we can actually say how well does this protein 21:57 transcribe dna and we can also assess its ability to bind to mitochondrial dna 22:03 with the idea that if it can't bind to dna it probably can't do its job in transcription as well 22:11 and so what we found from this work when we made these phosphorylation mimics of this protein is that when tf2m was 22:19 phosphorylated it didn't bind to mitochondrial dna as well and we've got a loss of mitochondrial dna 22:25 transcription so we pull tfb2m away and we no longer can transcribe dna 22:32 i have one data slide so this is it so i'm going to show you so you got to bear with me okay um so here's here's just 22:38 some of the data that support that picture that i showed you on the last slide so we have a template of a dna we give 22:46 it some mitochondrial dna in a test tube we give the system mitochondrial dna we give it our tfb2m protein the polymerase 22:53 and we say are you doing transcription or are you not doing transcription and so we can do these experiments we'll 23:00 just focus here on some of the things i have in boxes so what this is called is called a 23:05 runoff experiment and so that means can the polymerase start at the initiation 23:11 site for transcription and can it run off the template that we give it and so if we see dark dots it means yes 23:18 indeed it did run off and so for example wild type wt here we see two dark dots 23:24 um that means it did really well for serine 197 we see two dark dots that 23:29 means it did really well for these two other mutants phosphorylation mimics we see no dots right so it didn't do as 23:36 well um and so those are the data that kind of tell us that when we're looking at 23:41 t184e this phosphorylation site or the phosphorylation site at threonine 313 we 23:48 don't get productive transcription we can back up these claims 23:55 with some binding data so there's a lot going on here i'm not going to talk about it all i want you to focus on just a few things here so 24:02 if the protein doesn't bind to mitochondrial dna we would say that transcription likely will not occur and 24:08 indeed that's what we found in this study where transcription is not occurring and we 24:14 can look at how well each of the proteins binds to mitochondrial dna using a method called fluorescence 24:20 polarization the higher the number the worse the binding so we see high number here 455 24:26 nanomolar high number here compared to our wild type protein and so all of this kind of 24:31 nicely works together to say that we don't have binding and we don't have transcription 24:39 so what's next well what's next i showed you those other two kind of protein friends that are absolutely required for 24:44 transcription they have a bunch of modification sites we're kind of systematically working through those 24:49 proteins doing very similar experiments to what we showed here to say the core transcriptional machinery those three 24:55 proteins what are post-translational modifications doing what's next so mitochondrial dna again 25:03 binds to all these proteins right and i said i'd love to know what all 60 of these proteins are doing in 25:08 transcription so we know that there's proteins that are involved in packaging mitochondrial 25:13 dna they're called kind of nucleoid proteins it involves the core transcriptional 25:19 machinery which we're already studying and a number of really cool metabolic enzymes and so we're moving on we're not 25:26 moving on but moving into uh studying some metabolic enzymes as well 25:32 as we finish kind of understanding the transcriptional machinery the core transcriptional machinery 25:38 with the idea that these metabolic proteins are acting as sensors or rheostats of metabolic state and then 25:44 conferring that information to the mitochondrial genome to either enhance transcription or to decrease 25:50 transcription 26:00 okay i won't even touch it all right so that kind of ends uh that ends a little research portion we'll move on into the 26:07 third portion here maybe we will maybe we won't we'll see 26:13 i don't know if we have a bad hdmi or what 26:21 okay well it could be we're just going to leave it because it's working so so we're returning to the mug right okay 26:27 and andy kind of talked to you a little bit about this in the in the vocational biography that he uh presented at the beginning but i 26:34 entered the continuum program thinking man i really want to find this explicit connection between my faith and my 26:40 scholarship and i really wanted that i looked to lots of great people at hope doing research and can see kind of those 26:47 explicit connections and thought that's what it means to be a christian scholar there must be some explicit connection 26:52 that i haven't yet found for my work so i was frustrated the first two days of continuum because i'm like this isn't 26:58 helping i'm not finding anything and then we read something and a student 27:03 stopped by and gave me this mug with a nice little note and this student wasn't the best student 27:08 it was actually a student who took biochem one twice um with me so you i don't often think of getting 27:14 gifts from students who've had to take my class twice uh and this student the second time i was just a cheerleader i 27:21 wouldn't say that i went out of my way to do anything extra except to really acknowledge when they 27:26 were succeeding um and i think that that helped that student and the student did 27:32 fine and then ended up electing to take biochem 2 with me and so that really like spoke a lot and 27:38 then the student came back a year later and gave me this mug and told me they're off to a master's program 27:43 and stuff like that and kind of that along with some of the readings kind of came up at the same 27:49 time and i thought and maybe it doesn't have to be explicit and maybe through mentoring and the 27:54 relationships that i have with students and my colleagues i can demonstrate my faith in this way which is kind of 28:01 defined as purely vocational christian scholarship and so i think that through mentoring i 28:07 can really demonstrate my faith i can interact with people in ways that kind 28:13 of reveal to them things about my christianity and it's not always in 28:19 an explicit manner so again mentoring these are some of the awesome students i've had the privilege 28:26 of mentoring during my six years at hope in my lab these are the students that i 28:31 form the closest relationships with and get to have all sorts of interesting conversations with whether it's about 28:37 science or life or whatever and i feel very fortunate that these students are almost an extension 28:44 of my family and i still keep connected with my very first research student i was invited to her wedding in the fall 28:51 um and so those are types of relationships that i think being at a place like hope being a christian 28:56 scholar um a lot kind of afford these types of long-term connections 29:02 with students and i hope that through my mentoring i'm also kind of getting them to the places uh where they hope to be 29:10 so mentoring is certainly one aspect of of my vocation and my work as a 29:16 christian scholar but i do think that in the background i have questions 29:22 that um [Music] that kind of are related to origin of life questions and the mitochondria play 29:28 a very critical role in life right if we think back to the star wars video um 29:34 right they are kind of required for life as we know it and so i think as a 29:40 christian i'm very interested in this idea of both kind of science and faith and how they work together as and i 29:46 think that stems back to the origin of life right like where did this happen how did it happen um these 29:52 types of questions and so i'll talk a little more science here for a second so here we see an image of a 29:59 cell very very right early in evolutionary time some event occurred 30:06 in which a bacterial cell a proteobacterium was engulfed by some 30:12 sort of early eukaryotic cell so our cells are called eukaryotic cells human cells our eukaryotic cells 30:18 so this proteobacterium kind of was co-opted or engulfed by this early 30:24 eukaryotic cell and over the course of evolutionary time we get to the cellular 30:30 ancestor of kind of our cells without this event without kind of this symbiotic event of 30:38 the engulfment of this early mitochondrial precursor life as we know it probably would not exist right 30:45 because we need the atp that these things provide in order for the cell to continue to 30:51 grow and to become more complex et cetera et cetera and so i think in the back of my mind 30:57 this is really interesting and something that i like to think about even though i don't explicitly study 31:03 this connection and i'll just take this a little bit of step further to try to explain so again 31:09 at one point we had this ancient bacterial-like cell and over the course 31:14 of evolution we got to our mature mitochondria that we know of today 31:20 now over this evolutionary time scale lots of things happened and so some of the things that happened 31:26 was that information was transferred to the nucleus so at one point this was free living and it 31:33 did all the things that it needed to do on its own but i already emphasized the point to you today that the mitochondria 31:40 in our cells now cannot function independent of the nucleus it relies on this information within the nuclear 31:46 genome in order to function so these proteins that are encoded by 31:52 the nucleus and carried there but again throughout evolutionary time scales we're talking billions of years 31:59 it also the mitochondria also retained this small genome why 32:04 why did it want to retain this small genome and that to me is absolutely fascinating that the mitochondria has 32:11 this this genome that encodes only 13 proteins but it gave everything else up 32:16 to the nucleus if i knew how to study that i would but i don't but i think that that idea is kind of 32:23 really interesting and again brings in this perspective of faith and origin of life 32:28 that kind of drives an interest in the mitochondria and so i'll just end with a few quotes 32:33 the more i study nature the more i stand amazed at the work of the creator and so i hope that today i've given you a 32:39 little bit of a sense of that through my work and through my scholarship um i stand kind of amazed at that all of 32:46 this works how does it work right how does metabolism all work it's so complex 32:52 um how did this mitochondria come to be and why did these things get kept in the 32:57 mitochondria where other things got shipped away and so this is a quote by louis pasteur 33:03 which another kind of interesting connection here louis pasteur was one of the first scientists who 33:08 figured out that if there was oxygen around things grew better and so that's all about the mitochondria mitochondria 33:15 rely on oxygen to generate atp and i'll end just kind of with a few 33:20 more things about my thoughts on science and faith and so um there are people who are much more 33:26 articulate about this than i am and so i'm stealing from them for this presentation but someone so science and faith is 33:33 always this thing right and we can think about that when we think about origin questions we think about when we think 33:38 about evolution questions um and it seems like sometimes those things are at odds with each other 33:44 and i left probably undergrad thinking those two things were at odds with each other and i didn't know how to reconcile 33:50 it for myself i still don't but i'm kind of comfortable with that but i remember turning to some books and 33:57 one book that really helped me kind of put this put these pieces together about like i 34:03 can be a scientist and i can also be faithful francis collins you may know him he 34:09 just up until last month was the director of the nih he certainly has become much more in the public scene 34:15 with covid and things like that he was the leader of the human genome project the project that sequenced all of the 34:21 dna within a human and he writes in this book the god of the bible is also the god of the genome 34:26 he can be worshiped in the cathedral or in the laboratory his creation is majestic awesome intricate and beautiful 34:33 and it cannot be at war with itself and so i resonate uh strongly uh with this 34:39 kind of quote and just kind of stand in awe of what's happening in our cells at an atomic level 34:46 um he further says in another quote it's a miracle that it speaking of the universe has order fine-tuning that 34:53 allows the possibility of complexity and the laws that follow precise mathematical formulas contemplating this 34:58 an open-minded observer is almost forced to conclude that there must be a mind behind all of this 35:05 a profound truth that lies outside of scientific explanation and so i've used 35:10 the puzzle analogy already and for me i think there's lots of puzzle pieces that we as scientists can put together 35:16 they're probably always going to be like that piece that slid way under the couch or went down and you know my daughter 35:22 loses puzzle pieces all the time that we can't put the puzzle all together 35:28 and so to me that says there's something greater um that there's a greater mind behind all 35:34 of this that kind of has helped this to occur so with that i will end um and i'll just 35:40 say a quick acknowledgement um to all of my lab members folks listed there some collaborators 35:46 funding for the projects and then again to the continuum scholars program and to hope college for this experience 36:00 thank you kristen thank you for providing a presentation so wonderfully accessible to those of us 36:10 yes 36:22 um i will sometimes it works 36:33 you should be good 36:42 so i can understand that evolution works very well 36:47 on dna in the nucleus how does it work on dna in the mitochondria 36:55 if it's not doing the whole meiosis thing like it does in the nucleus or does it 37:01 does that question make sense i think so i i don't know i think so 37:07 so you're saying over time evolutionary time our dna has been altered within our 37:13 nucleus what happened in the mitochondria right because because i can if if 37:19 cellular information right in the dna moves to the nucleus then it there can use sexual and other 37:27 mechanisms to let evolution select on it and improve its function 37:34 does that same mechanism occur in the mitochondria is there meiosis and recombination there 37:42 no there aren't though i have never thought about that question that's a very interesting question no those types of things do not occur in the 37:48 mitochondrial genome um and i don't i don't know the answer to 37:54 your question so i i'll i'll try to answer it but it might not be perfect so that's what i'll say um and i don't know 38:01 a ton about this but so the mitochondrial genome again is inherited by the mom through maternally lin 38:06 through the maternal side and it's there are multiple copies of 38:12 mitochondrial dna within even a single mitochondria in development there's what's known as 38:18 the mitochondrial bottleneck where the amount of mitochondrial dna actually like kind of shrinks 38:24 um we'll go with that the amount of mitochondrial dna is 38:29 actually limited and then it's also just from the mom so we only want kind of the best mitochondrial dna to then go on 38:37 in the process of development and so that in some way kind of limits what 38:42 happens at least kind of in sexual reproduction about how the mitochondria are inherited 38:48 and things like that um but no the other events that you're talking about don't happen the mitochondrial dna 38:56 is kind of susceptible to damage that occurs within the mitochondria um but again we try to limit that with 39:02 limiting the amount of mitochondrial dna before kind of things take off that's the best i can do okay i think 39:09 that it certainly makes sense that with all the high energy stuff going on in the mitochondrion 39:15 you want to keep sensitive things outside thank you yeah 39:27 i have a much simpler question okay great but first let me thank you for your 39:32 presentation that clear metaphors were uh really helpful to somebody like me my 39:37 name is dave and i'm in the psych department but long ago in the early 1960s i was a 39:42 chemistry major and i was a biology minor unfortunately i've forgotten most of what i learned although i've stayed with 39:49 me what i've learned is my scientific empirical orientation to life but i don't recall learning about mitochondria 39:56 when did we come to appreciate mitochondria and their importance my goodness 40:02 that's definitely not an easier question i should know the answer to that but i don't know early 1900s i'm looking at leah i don't 40:10 know do you know yeah i don't know how long ago were you in school dave 40:16 i would definitely say people knew about the mitochondria before then 40:26 70s yeah yeah i mean i think the a lot of details 40:31 probably about the mitochondria i'm going to say 60 to 70s is like the um thinking about electron transport chain 40:38 and atp synthesis like those details um but linus pauline is certainly way before that and he kind of was one of 40:44 the first to start to think about these things but yeah that's a good question i don't know those states 40:52 yeah jason 1857 okay 41:03 thanks chris that was really great um my question will belie my biological ignorance because i was even younger 41:09 than david it wasn't longer ago but i was younger i was 13 the last time i had any of this kind of content um 41:15 are there still eukaryotes that don't have mitochondria or like we like that 41:20 it was you know once we have mitochondria all eukaryotes now have them yes correct 41:26 that event occurred early enough in evolution that you know fungi so having them wins out 41:32 over not having them yes 41:42 thank you okay so you said way long ago 41:47 a bacteria was like engulfed by the cell which is now the mitochondria but now 41:52 the mitochondria is like interdependent with the nucleus in order to do anything how much has like the 41:58 mitochondria really changed then like throughout that evolution to the point where now it almost like relies on the 42:04 nucleus to be able to do what it once did on its own yeah that's a really great question and and i don't again i 42:09 don't have a great answer that question because i don't think anyone really knows like what that early proteobacterium 42:16 exactly was maybe people know more than what i'm saying but certainly lots of genetic 42:23 information changed lots of regulation change right the mitochondria actually have probably developed more functions 42:30 than what they had when they were kind of on their own um we're you know we're talking 42:35 billions of years here of things that can happen and occur um so i don't know the explicit you know this pathway now 42:42 the mitochondria has and it didn't but i would envision that there's a lot of things happening with them 42:47 within the mitochondria that didn't happen just in that bug i would say that bug probably made a lot 42:53 of atp right and and then things kind of went from there 43:03 i have a question about i know you study how mitochondrial work and not how they don't work um but i know that in dance 43:10 marathon coming up in a month or so we've got at least one maybe more kids that have a mitochondrial dysfunction 43:16 which means sometimes it doesn't work out right and i'm thinking i know very little 43:21 again ignorance i'm laura i have no academic affiliation i'm staff so i'm 43:27 very ignorant on all of this but how does um if it is coming only from the mother and 43:34 i think of recessive and um genetic traits and you know the very basics that you learn 43:40 in middle school how does a mitochondrial disorder appear is it a 43:47 new mutation within the mitochondrial dna or is it is it how the mitochondrial interact with something else do you know 43:54 anything about disease causing mitochondrial dysfunction yeah so most of the diseases that children are 44:00 afflicted by that we're probably thinking about in this case not in the context of a thing like a cancer a 44:05 neurodegenerative disorder which is kind of a long-term decline in mitochondrial function those 44:10 disorders are oftenly often point mutations single nucleotide mutations 44:16 within the mitochondrial dna that then impact energy production so commonly 44:21 children will have kind of brain issues issues in tissues that use a lot of energy brain heart skeletal muscle 44:29 we see issues with children it you know comes out in children so those mutations can be inherited 44:36 through the mom um or they can be kind of mutations that occur sporadically all 44:42 mutations are random so a random event that occurs and it's in a really bad spot right in that really bad spot then 44:49 causes the disease but it can it can be inherited now there are other diseases 44:54 that are caused by the other things that you mentioned there but the ones that we see in children are how i just explained 45:01 i i have a question and that is maybe kind of tagging off of this what what are the implications of trans 45:08 mitochondrial transcription research going forward in other words as you do this research is it simply to advance 45:15 the biological science are there other implications for disease or yeah that 45:20 kind of thing as you do this research yeah that's a really great question so um 45:25 i think my goal really is let's understand how the mitochondria function better right 45:31 we don't know these pieces of the puzzle let's try to understand them and through understanding the transcription perhaps we can then 45:38 understand further how the cell works right a bigger question of biology 45:44 there are some human disorders that are caused by defective mitochondrial dna 45:49 transcription um kind of just these random energy issues uh 45:54 we looked at a paper this summer with the mitochondrial polymerase having some mutations and issues that led to its 46:01 dysfunction and so there are some one-off cases where we see these types of things in human disease 46:07 and so there are implications there but really our route is to try to understand 46:13 basic biochemistry that helps us understand how a cell functions 46:20 oh one more here can i yes let me let me 46:27 thanks i don't i don't really have a question i just wanted to comment i appreciated your um discussion of how you combine your 46:34 faith with your teaching so i'm not a biochemist but i do teach a a class to 46:39 kinesiology students who don't have a lot of chemistry it's called regulation of human 46:45 metabolism and we learn about all of the pathways and how we're able to provide atp 46:51 for sprinting compared to you know prolonged exercise and and one of the ways that i try to 46:58 um combine my faith with the concepts that we talk about is all the intricate ways that the 47:05 different enzymes and the pathways work together and and ask the students constantly you know why does it make 47:11 sense that it works this way to allow this pathway to work whereas that pathway doesn't work when this one is 47:17 working and i think the students um appreciate that so i 47:22 we don't go into any of the details um with structures or or 47:27 really bonding or anything with the students but i think just thinking of the big picture of how 47:35 god designed our bodies to work the way they do it is a nice way to do it so um and then 47:42 dave i think um when i teach about this it was 1967 that the mitochondria the we 47:48 learned about the electrochemical gradient and how that allows oxygen to be used so maybe that's why 47:55 you didn't learn that so much in the 60s anyways 48:00 thank you dr meyer would you like to have the last question here 48:07 it's kind of a stupid naive question but is there a boundary between biochemistry and microbiology and if so what is it 48:14 and which side of that boundary are you on yeah i think we i think as scientists we start to use 48:20 these terms a little bit loosely but i my definition would be a microbiologist studies 48:26 bacteria and yeast um and things like that whereas as a 48:31 biochemist where biochemistry is probably the biggest umbrella where people take that into different places but as a biochemist one might be a 48:38 protein biochemist like what i am i study protein function molecular interactions functions of molecules 48:44 within our cells those types of things so that's how i would loosely define those two things 48:51 well i want to tell uh you again christian thank you for a fantastic lecture today it was a delight 48:58 to have you be a part of our group this summer i your your thoughtful reflection here on your 49:03 own scholarship your faith was characteristic of conversations we had this summer and i just really appreciate 49:10 the time with you and you sharing with us today can we thank doctor didn't help from read again please thank you Hope College 4.95K subscribers Videos About Instagram Facebook TikTok LinkedIn 0 Comments rongmaw lin Add a comment...

The LIFE of BACH - Full Movie - English Narration & Subtitled

The LIFE of BACH - Full Movie - English Narration & Subtitled Ray Vandenburg 55.2K subscribers Subscribe 1.5K Share Download Clip Save 59,005 views Dec 8, 2024 It is recommended that you watch this updated version from "The Life of Bach". • "THIS IS MY LIFE" - JS BACH - UPDATED... Watch and listen to the most dramatic events in the life of one of our greatest composers, Johann Sebastian Bach. The film highlights his youth and education, his love for making music and composing; his marriages and children, the nice and nasty employers, his struggle against hatred and envy, his brutal end, his funeral, but above all his love and support for his family. The film is English-spoken and subtitled. To maintain authenticity, the German characters speak in their native language, with English subtitles. CORRECTIONS TO THE FILM: === The portrait at 02.50 is now showing Maria Elisabeth Lammerhirt === The photo at 46.08 shows now Gottfried Silbermann === The photo of Georg Böhm is now correct. === Eisenach is a city (not a village) === The profession of Johann Ambrosius Bach is now correct === The microphone on the preacher's desk has been removed === Bach now says "Zippel bassoonist" === No more modern pianos, just harpsichords === The photo of Dietrich Buxtehude is now correct. === Several smaller overall correctios are implemented. The above adjustments have been incorporated into the released film version: • "THIS IS MY LIFE" - JS BACH - UPDATED... I thank everyone who pointed out the errors and omissions. Ray Vandenburg, video editor. Music 5 songs Prelude and Fugue in G Major, BWV 541: II. Fugue Stefano Molardi J.S. Bach: Complete Edition, Vol. 10/10 The Well-Tempered Clavier, Book 1, Prelude & Fugue No. 1 in C Major, BWV 846: I. Prelude Sviatoslav Richter Bach For Relaxation C Major Prelude Bach C Major Prelude Piano Concerto in A major, BWV 1055: I. Allegro Martin Stadtfeld Bach: Piano Concertos Vol. 2 Weinen, klagen, sorgen, zagen, BWV 12: I. Sinfonia Netherlands Bach Collegium & Pieter Jan Leusink J.S. Bach: Weinen, Klagen, Sorgen, Zagen, BWV 12 Music Transcript Follow along using the transcript. Show transcript Ray Vandenburg 55.2K subscribers Videos About 226 Comments rongmaw lin Add a comment... Pinned by Ray Vandenburg @rafikbaladi6555 2 weeks ago This testimony is simply ravishing, moving, inspiring and refreshing for music lovers. And, with J.S Bach, it gives him justice whether with the richness of baroq decor or the warmth of his devotion to his family. 35 Reply Ray Vandenburg · 3 replies @pobinr 10 days ago "Bach is the most stupendous miracle in all music" Wagner 31 Reply @markjacksonturner6462 9 days ago He always wrote, "Soli Deo Gloria" on his music. To God alone be the glory. † 19 Reply 1 reply @stephene.robbins6273 5 days ago "Bach was very productive." Understatement of the century. 9 Reply Ray Vandenburg · 1 reply @viewlesswind 5 days ago Bach had enriched all of us. Thank you, Mr. Bach! 4 Reply @spmoran4703 2 weeks ago My favourite composer . Thank you . 17 Reply @julesmarwell8023 2 weeks ago the whole world owes Germany a great debt for giving us J S Bach. Thank you,. this said by an Proud Anglo 22 Reply 2 replies @stephanebelizaire5063 2 days ago 🌹Vivat Herr Bach ! 🌹 2 Reply @SebastianAnonymous-p2d 9 days ago A Slovenian "joke." When Mozart ascended to heaven he was met by St. Peter who said he was commissioned to take him directly to God. An overwhelmed Mozart could hardly contain his emotions upon being presented to God who astounded him even further by offering the position of Kapellmeister. Mozart said but was unworthy of that position and said surely it must go to Bach. God replied: That is impossible because I am Bach. 22 Reply 2 replies @leonardzane 3 weeks ago You have rendered the multifaceted genius, exhilaration, freshness, and heroism of the greatest composer! Congratulations on your feat! 29 Reply Ray Vandenburg · 4 replies @kenboydart 9 days ago At first I was very skeptical of this format to tell a story but I quickly got hooked. This production is beautifully done and I'm happy to learn more about the great masters work, thank you Ray . 14 Reply Ray Vandenburg · 4 replies @igorrromanov 2 weeks ago One of the greatest... THE GREATEST! THE GOD OF MUSIC! Though I consider GFHandel greatest too... but Bach IS the first and the foremost. 17 Reply @alanc3134 7 days ago Of J.S.Bach’s 20 children, only ten survived to adulthood; 5 boys and 5 girls by my calculations. This is typical of the child mortality rate in those days. However 4 of the boys became renowned composers but it is sad the girls were never provided the opportunity to explore their musical talents. Had females been given the same treatment as males, the baroque/classical repertoire could have been twice as rich! 4 Reply Ray Vandenburg · 1 reply @ariadne.thread 5 days ago For German speakers, the machine translations from English into German are often hilarious, and the language spoken is way too modern, but indeed this format is charming 5 Reply 1 reply @igorrromanov 2 weeks ago Actually, Bach remains were found since then following one particular clue: The year of his death only three citizens of Leipzig were buried in oak coffins. The rest got pine coffins, which, of course, soon disintegrated. All oak coffins were numbered. The remains of the man, quite tall, with strong built and large head correspond to the characteristics of Bach's physical appearance. 11 Reply @bruceweaver1518 2 weeks ago “The most stupendous miracle in all of music!” ——Wagner 9 Reply @dawnsonntag8443 7 days ago Um...in this film, little Sebastian was singing the Gounod arrangement of his work. That is indeed miraculous. 8 Reply @aksuli1 13 hours ago Thank you for the story! I couldn't keep my eyes dry. 1 Reply Ray Vandenburg · 2 replies @GTrotter-ho8ul 2 weeks ago Thank you. This brings new understanding and appreciation to my admiration of Bach and his inspirational music. 5 Reply Ray Vandenburg · 1 reply @mikekeyes6102 2 weeks ago Beats me why a grand piano was often shown which didn't exist at the time; it should of course been a harpsichord. 25 Reply 5 replies @alf5835 8 days ago Very well done. 3 Reply @pauldavies5611 7 days ago This was fun to watch and quite instructive. Also enjoyable was the dialogue in the German language. I would only suggest more substantial discussion of the music. The Well-Tempered Klavier is not mentioned and it would be nice to know a little more about the Masses, the Brandenburg Concertos, the famous Chaconne for violin in d minor. But thank you very much for putting this on for viewing. 5 Reply @CanuckBeaver 1 day ago Excellent program, very professional appropriate to Bach. Everyone was treated with respect, each with their own stories and reasons. 1 Reply Ray Vandenburg · 1 reply @HolyGrailArts 1 day ago Wonderful documentary. Thank you for sharing. J.S.B. whom I consider the supreme Architect of music, other than Beethoven and Georg Friedrich Händel, was once employed at the court of my maternal ancestral relative, King, Friedrich II "The Great" House of Hohenzollern. Kind Regards from the Viceroyalty of New Spain. Álvaro Guevara y Vázquez, Composer BMI. 1 Reply Ray Vandenburg · 1 reply @jkgou1 2 weeks ago Thank you very much I try to understand his works through his life This film is vivid, concise, and very helpful Merry Christmas Happy 2025 6 Reply Ray Vandenburg · 2 replies @johntibbetts7766 5 days ago In the second minute of this film, the narrator states that Bach was born on March 31, 1685. Any source on Bach's life will report that he was born on March 21 of that year, not March 31. It turns out that March 21 is my birthday, and I've always celebrated my birthday toasting Bach, my favorite composer 2 Reply Ray Vandenburg · 1 reply @javierblanco7478 2 weeks ago DIVINO BACH 4 Reply @peterquennellnyc 2 days ago (edited) Thanks Ray! Well done. Clever, entertaining, respectful, and very touching in parts (repetitive nitpickers here; are they AI?!) I was in Bach's cathedral soon after The Wall came down. Bach was never a biggie for the Communists. In the evening, there wasn't a single other person around. In the morning, there was just one young woman, practicing on the vast organ at the back (there are two); memory of her still touches me. There is a Bach gravestone right in the middle of the nave; no flowers on it then, but always covered now. 2 Reply Ray Vandenburg · 1 reply @shanti9040 8 days ago (edited) Awesome...excellent....beautiful ..👏👏👏💐💐💐💐💕...I LOVE..J. S. BACH.🌹❤❤❤❤❤❤❤..thank You ..💐💐💐💐💐💐🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏.. 2 Reply @dietermarczinski5516 3 weeks ago Dank je wel beste Ray. Ik verheug me echt op de Duitse versie. Ik wens je een fijne adventstijd en groet je hartelijk uit Zwitserland die, Dieter.🌷🌷🌷 3 Reply @64storun 17 hours ago You made my day. Thank you very much. Reply Ray Vandenburg · 1 reply @pantelismisikos9023 2 weeks ago Nice work!! 2 Reply @SebastianAnonymous-p2d 8 days ago I'm sure you know that Bach greatly admired Vivaldi for what he regarded as his multi-dimensional frivolity and exuberance that in some ways is found in Bach's secular cantatas (see the fantastic Netherlands Bach Society's rendition of the Kaffeeekantata) and, of course, in his harpsichord concertos particularly BWV 1065 an incredible version under the aegis of Alexandra Korenva). Bach added much stronger counterpoint and depth. I think that Vivaldi always started a work with a brilliant display of fireworks that he found difficult to maintain and Bach was able to extend that in my view. Good luck with the project. 3 Reply Ray Vandenburg · 3 replies @yacovmitchenko1490 8 days ago (edited) The GOAT for me. Sorry Mozart and Beethoven. 1 Reply @WarinPartita6 2 weeks ago (edited) Big​ ​thanks.​❤😂🎉😅😊😂❤ 5 Reply Ray Vandenburg · 1 reply @brads2041 3 days ago Very good job. This must have been a lot of work 2 Reply Ray Vandenburg · 1 reply @donaldturnbull 7 days ago Ignoring the historical flaws like a piano that didn’t exist in Bach’s time it was a decent movie. 3 Reply @markaaronsite 6 days ago bravo. wonderful accomplishment! Music selection, visuals and the story all made for superb entertainment. Papa Bach will always be the Shakespeare of music and our divine muse. 4 Reply @timcrockford1731 3 days ago Bach at Lüneburg from 1700-1702. Johann Sebastian Bach first met Johann Adam Reincken in 1705, received lessons from him from 1705 –1706, and met again in 1720. Fantasia and Fugue in G minor, BWV 542: The Fantasia may have been composed separately during Bach's time in Köthen (1717–23). Probable dates given for the Fugue being improvised in 1720. No autograph manuscript of either the Fantasia or the Fugue survives, and no manuscript of the Fantasia survives from the composer's lifetime. During his 1720 trip to Hamburg Bach is believed to have met Reincken,[3] whose music he had known since his teens. (See Wikipedia) Reply @johanvanham5102 3 weeks ago Bach is not my favorite,but I enjoyed the video very much. Must have been a hell of a job to select so many details of sound and images of Bach's dynamic life in a video not too long nor too short. Guess a process of many many weeks . Saw the German version as well, glad you made an English one as well. Thanks a lot Ray 6 Reply Ray Vandenburg · 2 replies @axiaocy 2 weeks ago Bach did not compose any symphonies, he composed orchestral suites. 7 Reply 1 reply @EdeltraudGurtner 2 days ago Sehr gut gemacht, sehr unterhaltsam. Reply Ray Vandenburg · 1 reply @rineric3214 2 weeks ago Bach did NOT walk around with a sword on his body. He was wearing a WOODEN "sword" that was a stage prop he had just worn in a musical play at a nobleman's mansion. 5 Reply Ray Vandenburg · 2 replies @williamconklin5017 12 days ago If somebody could prove to me that Bach was God, I would believe in God 8 Reply 2 replies @malcolmledger176 2 weeks ago (edited) Amusing how Bach calls Anna Magdelena "honey" at 42:55. And with all the organ music Bach wrote, why keep repeating the same pieces? Your painting accompanying the name of Gottfried Silberman (45:50) is in fact of Frederic the Great of Prussia. 6 Reply @igorrromanov 2 weeks ago NOTE: In place of Buxtehude the picture shows Alessandro Scarlatti. 2 Reply Ray Vandenburg · 1 reply @pines3200 2 weeks ago So many pieces NOT even written by Bach in the first four minutes. Agh! 5 Reply @wamexart 5 days ago Thank you for such a moving and comprehensive rendering of JS Bach life. Using harpsichord for the keyboard parts that were played in the piano, would have contributed to the fidelity of the movie. 1 Reply Ray Vandenburg · 1 reply @Ben777World 11 days ago A delightful work. I was worried about the historical accuracy, but your corrections show you take this seriously. Reply Ray Vandenburg · 4 replies @suzanneozorak1212 2 weeks ago Piano did not exist then 3 Reply @SebastianAnonymous-p2d 10 days ago Whilst Bach played an early version of the pianoforte he never composed with that instrument which he said lacked perfection. He worked with clavichords and harpsichords. A pity this has not bee respected in the film. 1 Reply Ray Vandenburg · 1 reply @peterquennellnyc 2 days ago Ray, I offer you this (my original comment under an excellent Messiah video) for if/when you turn to Handel. This is the astonishing critical-path longshot which resulted in the Messiah. Handel had to be born in Germany, of a father then over 60, had to have his mother secretly buy him a piano, had to have a local count hear his organ playing (at age 11) and sponsor him, had to learn baroque music and Italian in his 4 years in Italy, had to find cities & audiences back in Germany smallish, had to move to London for more business, had to be a favorite of the German-born kings then, had to see his main income from Italian operas fading, had to have an avid Christian come up with the Messiah's unique libretto (phrases all lifted from the bible), had to have a sudden health problem but survive it, had to get fired up enough to write the music for all singers and players in 3 weeks (at age 56), had to need money, had to be invited to Dublin, had to encounter a certain high-profile contralto, had to grasp a charitable angle, had to encounter a crazy-keen first audience there, had to be rebuffed initially in London, and had to be championed years later by the passionate co-founder of a hospital for babies of unmarried mothers (eventually 25,000 of them) in large part funded because he made the Messiah in a real sense theirs. 2 Reply Ray Vandenburg · 2 replies @JohnvanderVeen-c3c 13 days ago Why music examples on piano? Bach composed for the harpsichord. 2 Reply Ray Vandenburg · 1 reply @reneblom2160 12 days ago Huh! Why is a picture of Constance Mozart being shown to depict the mother of J.S. Bach? This is just ridiculous - I'm out of here! 3 Reply @percivalpc 10 days ago I'm sorry, but I'm too terrified at the uncanny valley to watch this. 1 Reply Ray Vandenburg · 1 reply @alanc3134 7 days ago Stunning that both J.S and Mary Magdelena were buried in unmarked graves ; hard to believe but I think the same fate befell Mozart? Reply Ray Vandenburg · 1 reply @SEMPRELISZT 8 days ago There's some good info, but some silly anachronisms, like JSB playing on a grand piano. 2 Reply @BlossomedJewelsOfficial 8 days ago 38:49 Bach didn’t compose cantatas for the prince at that time because only secular works were required of him. He composed most of his cantatas when he was in Leipzig. Reply Ray Vandenburg · 2 replies @Jupper1958 10 days ago Good information content, but don't you find the graphics a bit on the kitschy side? 1 Reply Ray Vandenburg · 1 reply @malcolmledger176 2 weeks ago There were never any "piano concerts" at Zimmermanns coffee house. Never heard of the harpsichord? 2 Reply Ray Vandenburg · 1 reply @Frenchie1116 2 weeks ago Wait, that's Constanze Mozart at 2:50 🤨 5 Reply 1 reply @MrXiaoda 2 days ago The Bible of the classical music. Reply @michaelbookout7561 2 days ago thank you for this excellent presentation both informative and complete in history Reply @marklaverty3221 8 days ago A couple more errors: At 3:33, Bach attends the 'EisenBach Latin School'??????? I don't think so. He never went that far from Eisenach. Moreover, shortly after this statement, we hear the strains of 'Ave Maria' sung by a vocalist, presumably intended to sound like 'little Sebastian singing in a lovely, pure soprano voice'???? Nope. Bach never did/would never compose a song/prayer to Mary. That is a concoction by Gounod, composed more than 150 years after Bach's birth. And Bach was Lutheran, not Roman Catholic. Lutherans don't pray to Mary; she cannot hear nor respond to prayers. 2 Reply Ray Vandenburg · 2 replies @arthuroldale-ki2ev 2 days ago Enjoyed this video but was surprised to see that awful word AWESOME used ! 1 Reply Ray Vandenburg · 1 reply @valtergilenardi2639 7 days ago Amazing Video...Saluti from Lecce South Italy BaroqueCity. 🏖🕍⛪🌅. I am a Great Fan to the Kappellmeister of Lipsia 👑🎼🎹🎻🎺📯❤🎶🎶🎶😊 1 Reply Ray Vandenburg · 1 reply @suzanneozorak1212 2 weeks ago Gounod was born much after—?! 2 Reply @musictheoryforeveryone7938 3 days ago It seems even Artificial Intelligence is taking over the movie making process. It is reducing a lifetime of Bach’s music making and genius to a production that took some computer three seconds to spit out… While the individual scenes are striking, the overall effect is very disjointed and chaotic, lacking a cohesive style and characters consistent from scene to scene. 1 Reply Ray Vandenburg · 1 reply @mathersdavid5113 9 days ago Odd that there is no mention of several key works that define Bach's career in music- Well-tempered Klavier, Art of Fugue, Musical Offering, B minor Mass, St. Matthew Passion and others. His face keeps changing but rarely looks much like him for some reason. There are reliable portraits to aid with this aspect. Reply Ray Vandenburg · 1 reply @joyeux-o8v 5 days ago Would anyone know the title of the Bach composition played at 25:54 onward? It's a pity only a fragment of it is played. It's the tune I love the most in this whole documentary. Thank you for this post! It's beautiful and informative. Reply Ray Vandenburg · 7 replies @rinkinkel 6 days ago If this the best AI has to offer, I'm not worried. Reply Ray Vandenburg · 1 reply @nab626 9 days ago It is indeed a wonderful film. It would add immense value and listening pleasure if you would learn to pronounce the German names in German. I assume this is not an Ai generated voice. 1 Reply Ray Vandenburg · 1 reply @RobertMGoodman 8 days ago I would like to repeat an earlier comment that your painting accompanying the name of Gottfried Silbermann (45:50) is, in fact, that of Frederick the Great of Prussia. Reply Ray Vandenburg · 1 reply @hansaniarchibald6919 7 days ago What is the organ piece being played at 11:00? Reply 2 replies @paultah1 11 days ago (edited) The Genius of Bach, but what happened to his thorough studies nights without end, music of Vivaldi ,Marcello, Buxtehude, Couperin, Rameaux, Teleman, Scarlatti, Handel, before he was ordained as Genius? No mention of that Reply @johnwight6041 8 days ago Piano teacher? 1 Reply @SebastianAnonymous-p2d 9 days ago Thank you for your reply. Could I suggest that if you have not read the books by Christof Woolf about Bach or not seen the film made a long time ago by Jean-Marie Straub that they would supplement your exciting commitment to the life and work of Bach. I will certainly follow your suggestion abut looking at the latest version. Again, there are some important insights in the work of Charles Rosen that you probably have seen/read. Also and there probably no room in your film but in philosophical terms the relation between Bach and Kant is worth thinking about. As you shown there is immense depth and breadth to Bach. Again think you. Reply Ray Vandenburg · 1 reply @kalaimanidhan 8 days ago What software did you use? Reply Ray Vandenburg · 1 reply @dng88 2 weeks ago The death of Maria and no chance to say goodbye leave with us the most haunting violin solo music ever written. Strange not here. 1 Reply Ray Vandenburg · 2 replies @jimbo2629 2 weeks ago Very informative but why revert to the present tense every now and then. He died over 200 years ago. 1 Reply Ray Vandenburg · 1 reply @jsv438 7 days ago Why does it mention Bach being taught "piano"? There was no piano. I believe the narrator meant harpsichord, but that seems like an odd mistake since the word "piano" would have to have been written? I'm just wondering how that error got in there? ~JSV Reply Ray Vandenburg · 3 replies @rickblessing2447 6 days ago The guy they picked to narrate his life pronounces his last name Sebaaastion, like a sheep "baaaa"s. I like to think Johann would have gotten a laugh out of it. Reply 1 reply @volkerhl 13 days ago terrible! so much wrong!! 2 Reply @ansiedeswardt 2 weeks ago Horrible AI illustrations. FAKE. I love Bach but not this. That tokata is probably not even by him. There is Soooo much to choose from . 4 Reply @antoniobarrospelica7982 12 days ago This documentary IS terrible. Embarrassing, really. Childish in sort of bad way. 3 Reply @meijong6453 2 weeks ago Ridiculous and unforgivable to have piano playing. 5 Reply @hiltonmarlton6373 2 weeks ago Was this produced by AI? Full of gaping errors and misleading information. Not to be used for academic purposes. Like a bad TV production from the 1970s. 1 Reply @p.f.3141 9 days ago Born March 21st, not 31st. Reply Ray Vandenburg · 1 reply @pascalpatrick3009 3 days ago Why, for the concerti of Vivaldi, you choose a modern piano????? non sens again. There weren't enough recordings with harpsichordists available? :) Reply Ray Vandenburg · 1 reply @igorrromanov 2 weeks ago The infant death rate is appalling. Even in such an avanced conglomerate of German speaking countries... Reply Ray Vandenburg · 1 reply @jsv438 6 days ago And AGAIN with the piano! This time they have Bach playing a PIANO! There were no pianos, Bach didn't play a piano? With all this history about him, and it seems to be accurate, WHY would they ever show him playing a piano?!! ~JSV Reply Ray Vandenburg · 3 replies @Korea4Me 2 weeks ago OMG, I can't watch this. AI rubbish again and that poor horse at the beginning... 1 Reply @YQ2138 3 weeks ago 🎄❤👏😊dankeschon Reply @nadzienka696 5 days ago Why complicate the story in German? If it weren't for the narration in English, I wouldn't be interesting to me. Reply Ray Vandenburg · 1 reply @skulptor 6 days ago The horse and carriage belong in a horror film.. Reply @whukriede 12 days ago The German they let them speak is ridiculous, total failure. 1 Reply 1 reply @tonysouter8095 9 days ago (edited) "Father Ambrosius was an organist". Huh? No, a trumpeter. Please check before you start production. "Eisenbach"? That's plain stupid: "Eisenach". Why are there typos in the subtitles? Why use piano and not harpsichord, given the trouble you've gone to to recreate scenes in Bach's life? "Leipzigers set out to a PIANO concert"? The piano didn't exist. But there are some good things about this spoitl production. Reply Ray Vandenburg · 1 reply @ilyamurom 14 hours ago IA à tout faire et cela se voit 😢 Mieux vaut mille fois revoir "La petite chronique d'Anna Magdalena Bach" de Straub et Huillet avec acteurs et musiciens humains, pas des fantomes-pixel. Reply @jeannebonar4268 6 days ago to show the hammers of a modern piano is ignorant he had organs clacichords harpsicords the piano was later Reply Ray Vandenburg · 1 reply @stringsandpipes7548 2 weeks ago AI is garbage 17 Reply @TheCapital101 2 weeks ago Pretty lousy 2 Reply @dng88 2 weeks ago Tbh b is a whole family tree. J S … Reply @EPeltzer 1 day ago (edited) This mostly AI generated video has some of the most bizarre and unnatural facial animations I have ever seen. Also it seems that about a dozen completely different looking AI actors portrayed Bach. Musically speaking this is not very enlightening and some of the musical selections are just jarringly wrong, though I am certainly no Bach expert. However, a fair bit of research, thought and effort went into this video and I did learn quite a bit about the man's life and career and millieu. Reply Ray Vandenburg · 1 reply @BaroqueHarpsichord 2 days ago So many mistakes, where even to begin. If this is what ai has to offer then the future of humanity will be dumber than a box of rocks. Reply Ray Vandenburg · 1 reply @rogerevans9666 2 weeks ago @34:51 nice looking woman Reply @zallen5833 1 day ago did you NEED to use ai art Reply Ray Vandenburg · 1 reply @md88kg 1 day ago (edited) Please English speakers, quit pronouncing this revered name as BacK, it's BacH, as in Spanish baJo, ojala, ajo, 😀 Reply @pascalpatrick3009 3 days ago The story with Louis Marchand is false. Marchand was a very great French musician and organist recognized in his country. The music and the name of J.S. Bach were unknown in France!!!! Marchand did not see the point of traveling hundreds of kilometers to face a unknown stranger. To say that he was afraid is nonsense and especially very false. Reply Ray Vandenburg · 2 replies @tomestubbs 1 day ago Metal quill pens were not around at that time. Reply Ray Vandenburg · 1 reply @chrisprior2870 5 days ago Interesting, but highly flawed and too American for my taste. Reply @tonyhauserguitarist4080 11 days ago Weird 1 Reply @white8771 10 days ago I am sure they did not use such horrible Americanisms as gotten and okay. Reply @homolix 9 days ago 4:46...it´s a mistake: it should say: "harpsichord teacher". the piano has not even been developed. there were pianofortes but that instruments were not to popular yet. 1 Reply

Mark Zuckerberg' wife Made HUGE Announcement on Their Marriage

Mark Zuckerberg' wife Made HUGE Announcement on Their Marriage The Prime Expedition 65.3K subscribers 269,365 views Dec 27, 2024 Mark Zuckerberg' wife Made HUGE Announcement on Their Marriage Tech billionaires' marriages are breaking up, and this has been happening more often than we might imagine, and now, it seems like the recent couple who are in troubled waters are none other than Mark Zuckerberg and his wife, Priscilla Chan. Now, if you’re someone who follows their story and their social media posts closely, you’ll think they had the perfect family life. However, recent comments from Priscilla suggest otherwise. So what exactly did Priscilla say? And are the couples really on the verge of splitting up after 21 long years together? Join us as we get into all the tech details Disclaimer: The content presented in our videos is intended solely for entertainment purposes. While we may draw upon facts, rumors, and fiction, viewers should not interpret any part of the content as factual or definitive information. Please enjoy responsibly. How this content was made Altered or synthetic content Sound or visuals were significantly edited or digitally generated. Learn more Transcript Follow along using the transcript. Show transcript Transcript 0:00 people are quick to discuss Mark 0:01 Zuckerberg however the billionaire 0:03 Facebook Founder's wife has an 0:05 eye-opening story of her own here are 0:07 some things you may not know about 0:08 Priscilla Chan and then what year did 0:10 you meet Mark Zuckerberg I met mark four 0:14 weeks into college so you were going to 0:16 leave even though you had met 0:17 him Tech billionaires marriages are 0:20 breaking up and this has been happening 0:22 more often than we might imagine and now 0:26 it seems like the recent couple who are 0:28 in troubled waters are none other than 0:30 Mark Zuckerberg and his wife Priscilla 0:33 Chan now if you're someone who follows 0:36 their story and their social media posts 0:39 closely you'll think they had the 0:41 perfect family life however recent 0:44 comments from Priscilla suggest 0:46 otherwise it is 514 two people who used 0:49 to work in the home of Facebook CEO Mark 0:51 Zuckerberg and his wife risilla are now 0:54 suing the couple so what exactly did 0:56 Priscilla say and are the couples really 0:59 on the verge of splitting up after 21 1:02 long years together join us as we get 1:05 into all the tech details the unexpected 1:09 announcement Mark Zuckerberg the 1:11 Facebook founder has had a lot of 1:13 success associated with his name and his 1:16 Facebook invention has undeniably made 1:18 him a very rich man Mark Zuckerberg to 1:21 be the billionaire Tech Titan who 1:22 founded Facebook but while all of this 1:25 might be true it seems as though there 1:27 is one area in his life that isn't 1:30 performing so well his marital life and 1:33 that must really hurt his wife you know 1:37 the saying a happy wife is a happy home 1:41 it seems as though Zuckerberg's marital 1:43 life is far from happy and the reason 1:46 actually boils down to Zuckerberg's 1:48 over-the-top obsession with the tech 1:50 world and building the next it thing 1:53 that can attain as much success as 1:55 Facebook but it seems as though 1:57 Priscilla has had it up to her throat 2:00 because boy she made some shocking 2:02 revelations that made eyes go wide so 2:06 curious to find out what these shocking 2:08 revelations are stick around on social 2:12 media they might appear to be the 2:14 perfect couple with Zuckerberg showering 2:17 his lovely wife with lavish gifts 2:20 leaving fans gushing but you know what 2:23 they 2:24 say appearances can be deceiving and you 2:27 can never truly know what goes on behind 2:30 closed 2:31 doors now before we get into what 2:34 Priscilla said let's start here so as to 2:37 better understand why she said 2:39 everything she said the couple recently 2:42 celebrated their dating anniversary and 2:45 best believe that Zuckerberg went all 2:47 out for his lovely wife Mark Zuckerberg 2:50 celebrates his anniversary with 2:51 Priscilla Chan Mark Zuckerberg recently 2:54 hit the studio to set the bar higher for 2:56 anniversary gifts in a post on Instagram 2:58 on Wednesday the 40-year-old meta 3:00 founder and CEO announced a surprise for 3:03 his 39-year-old wife Priscilla Chan the 3:06 Facebook founder took a nostalgic trip 3:09 back to his college days sharing an 3:12 Instagram post on November 13th that 3:15 featured Lil John and the Eastside Boys 3:18 and ying-yang twins 2002 hit single Get 3:23 Low the song playing at the party where 3:25 he and Priscilla first met at Harvard 3:28 University in of their anniversary 3:31 Zuckerberg recorded a cover of the track 3:34 and even recruited hip-hop Legend T 3:36 Payne to join him on the 3:39 project the cover released under the 3:41 stage name Z Pan featured Zuckerberg 3:44 singing over an acoustic instrumental 3:47 with Tay adding his signature autotune 3:50 harmonies fans were treated to behind 3:53 the scenes images of the duo recording 3:56 along with throwback photos of 3:57 Zuckerberg and Priscilla during their 3:59 College 4:00 days can't get quite as low anymore but 4:04 more in love and grateful for that love 4:06 than ever Priscilla commented on the 4:09 post and that's not even the only lavish 4:12 gift he has gotten her back in August 4:15 Zuckerberg shared a post featuring a 7- 4:18 foot Roman inspired statue of Priscilla 4:21 commissioned in her honor better CEO 4:24 Mark Zuckerberg is showing his love for 4:26 his wife in turquoise and chrome he 4:29 posted a photo on Instagram behold 4:32 showing the 7 foot sculpture he had 4:35 created in the likeness of his white the 4:38 statue with its flowing metallic surface 4:41 and striking green tones stirred quite 4:44 the reaction 4:46 online bringing back the Roman tradition 4:49 of making sculptures of your wife 4:51 Zuckerberg captioned the post the post 4:54 caused quite a stir on Instagram as one 4:56 user said this is the most billionaire 4:59 thing to do ever the right woman turns a 5:02 boy into a real man another added wife 5:06 guy level Advanced one other quipped I 5:10 was literally about to get the same 5:12 thing for my wife now I have to Pivot 5:15 Priscilla herself even commented the 5:18 more of me the 5:19 better now while the post was met with 5:22 positive comments some had even negative 5:25 comments a couple's therapist named 5:27 Isabelle Morley a clinical psychologist 5:30 in Massachusetts told Business Insider 5:33 that such a lavish gift might be a cry 5:35 for help or even a signal that something 5:38 ugly is brewing in its head now in her 5:41 own words she called the lavish gifts a 5:44 red flag she further said that such 5:46 extravagant gifts can sometimes also be 5:49 an attempt to manipulate the receiver or 5:52 elicit a specific reaction from them was 5:56 it really just to show his deep love and 5:58 gratitude for her her and who she is and 6:01 who she's been in his life Morley said 6:05 or was it to make him look good to other 6:07 people and to get a certain affectionate 6:09 response from her now while we might not 6:12 know why Zuckerberg really got his wife 6:15 that sort of gift many people speculate 6:18 that it could possibly be because he 6:20 referred to Priscilla as human wife 6:23 crazy right so here's what happened back 6:27 in March Priscilla mentioned that her 6:29 husband has been acting quite suspicious 6:32 but not for the reason you might be 6:33 thinking he was using his Oculus far 6:36 more than usual and he started to refer 6:38 to her as his human wife coupled with 6:41 that he spends almost 14 hours on his VR 6:44 headset and in one instance when he took 6:47 off his VR headset he looked confused 6:50 and asked her if she was his computer 6:52 spouse or his flesh 6:54 spouse while Priscilla has had a 6:56 long-standing policy to not ask what her 6:59 billionaire husband did in the metaverse 7:02 she had to admit that she was starting 7:04 to wonder if they were safe with all the 7:06 time he was spending obsessed with his 7:08 metaverse Oculus but calling Priscilla 7:11 his human wife wasn't even the only 7:13 over-the-top thing he did that same 7:16 month he accidentally called their son 7:19 digital spawn and then made him confirm 7:22 he had legs and wasn't just a floating 7:25 torso Priscilla has admitted that she 7:27 didn't like her husband's obsession with 7:29 his VR and this might perhaps be a 7:32 further contributor to The Strain on 7:34 their relationship and him getting 7:36 wealthy people's gift for her following 7:39 the incident the Press did try to get 7:42 Priscilla to comment on the issue but 7:44 she couldn't be reached for comments she 7:47 also couldn't be reached for comments 7:49 after her husband reportedly attempted 7:51 to have intercourse with a meta Quest 3 7:54 headset this is the new meta Quest 7:58 3s High quality mixed reality for 8:02 $299 and was immediately 8:05 electrocuted we cannot imagine the site 8:07 and how horrified Priscilla must have 8:09 been having to see 8:11 that but long before virtual realities 8:14 and much controversial headlines their 8:17 story began in a much simpler 8:20 setting how the couple 8:22 met Mark Zuckerberg may not have left 8:25 Harvard with a degree but his time at 8:27 the prestigious University inter 8:29 introduced him to the love of his life 8:31 Priscilla 8:32 Chan their story began like every 8:35 College Romance when the two crossed 8:37 paths in 2003 at a party hosted by 8:41 Zuckerberg's fraternity Alpha Epsilon 8:45 Pi both were students at the time 8:48 Zuckerberg a sophomore already making 8:50 waves on campus and Chan a Boston native 8:55 in her second year you could say their 8:57 meeting was unconventional because 8:59 because they were both waiting in line 9:00 for the bathroom at that time Zuckerberg 9:04 was Notorious on campus for a prank that 9:06 nearly got him expelled the creation of 9:09 face Mash a website ranking students 9:13 attractiveness Priscilla admitted she 9:15 thought Zuckerberg's Antics might lead 9:18 to his dismissal from Harvard in fact 9:21 when they first met Zuckerberg himself 9:23 believed his days at the University were 9:26 numbered their first interaction as he 9:28 later recounts Ed involved him jokingly 9:31 urging her to go on a date quickly 9:33 before he was 9:35 expelled despite the unusual start their 9:38 connection 9:39 blossomed and on their first date 9:42 Zuckerberg in his characteristic 9:44 candidness told Priscilla that he would 9:47 rather spend time with her than finish 9:49 his take-home 9:51 midterm cute right while her type A 9:55 personality was initially taken aback by 9:58 what Zuckerberg said 10:00 it actually marked the beginning of a 10:01 love and partnership that would endure 10:04 the ups and downs of both their personal 10:06 and professional lives by 2005 10:10 Zuckerberg had officially dropped out of 10:13 Harvard to focus on building Facebook I 10:16 remember that night I launched Facebook 10:18 from that little dorm in Kirkland house 10:21 I went to noes with my friend KX and I 10:25 remember telling him clearly that I was 10:27 excited to help connect the Harvard 10:29 commity Community but one day someone 10:30 would connect the whole world he 10:33 relocated to paloalto California where 10:36 the platform opened its first office 10:39 Priscilla however stayed the course at 10:41 Harvard and graduated in 10:44 2007 Zuckerberg attended her graduation 10:47 to celebrate her achievement soon after 10:51 she moved to California to be closer to 10:53 him enrolling in medical school at the 10:56 University of California San Francisco 10:58 in 20 11:00 2008 despite their demanding schedules 11:03 the couple maintained a balance 11:06 Priscilla imposed a rule for their 11:08 relationship they had to have one date a 11:11 week and spend at least 100 minutes of 11:14 uninterrupted time together away from 11:17 Facebook their shared time was often 11:20 simple but meaningful they enjoyed 11:23 walking in the park playing bachchi and 11:26 engaging in Friendly races during rowing 11:28 outing 11:30 Sundays became a time for exploring 11:32 Asian Cuisine Priscilla supported 11:35 Zuckerberg during some of the most 11:37 stressful periods of his career 11:40 including when he turned down A1 billion 11:42 do buyout offer from Yahoo in 11:46 2006 she described this as the most 11:48 anxious she had ever seen him in 2010 11:53 their relationship took another step 11:55 forward when Priscilla moved into 11:57 Zuckerberg's rented house in Palo Alto 12:00 true to form Zuckerberg made the 12:02 announcement on Facebook humorously 12:05 offering their Surplus household items 12:07 to 12:07 friends that same year the couple 12:10 adopted a dog a pulley named Beast who 12:15 quickly became a part of their growing 12:17 family and by March 2011 they made their 12:21 relationship Facebook official and by 12:25 May of that year they had purchased a 12:27 five-bedroom home in Palo Alto for a 12:29 whooping sum of $7 12:32 million over time Zuckerberg expanded 12:35 the property acquiring the four 12:37 neighboring homes for an additional $43 12:41 million however the couple's wedding in 12:44 May 2012 came as a surprise to friends 12:47 and family Mark Zuckerberg's Facebook 12:50 status is now married he tied the not on 12:53 Saturday with Priscilla Chan his 12:54 girlfriend of 9 years in a surprise 12:57 ceremony in their paloalto California 12:58 back yard the event was disguised as a 13:01 celebration of Chan's graduation from 13:04 Medical School only for guests to 13:07 discover it was actually their wedding 13:09 day the ceremony held in the backyard of 13:12 their Palo Alto home was intimate and 13:16 unpretentious their honeymoon in Rome 13:18 reflected this Simplicity as well the 13:21 newlyweds were spotted enjoying a meal 13:23 at McDonald's during their 13:25 stay after their honeymoon Zuckerberg 13:29 continued to expand their real estate 13:31 portfolio purchasing a townhouse in San 13:34 Francisco's Dolores Heights neighborhood 13:36 in 13:37 2012 the property underwent a $1.6 13:40 million renovation before being sold in 13:44 2022 for $31 13:47 million meanwhile their Investments 13:50 extended Beyond 13:52 California in 2014 the couple acquired 13:55 over 13:56 1,500 acres of land in Kawai Hawaii for 14:01 more than $100 14:02 million further showing their presence 14:05 in the real estate market while Chan 14:08 pursued her medical career completing 14:11 her pediatric residency in 14:13 2015 that same year they announced the 14:16 birth of their first daughter Maxima Max 14:20 Chan Zuckerberg after years of trying 14:23 and enduring the Heartbreak of three 14:26 miscarriages to celebrate Max's arrival 14:29 the couple launched the Chan Zuckerberg 14:31 initiative pledging to donate 99% of 14:35 their Facebook shares to causes like 14:37 education and medical 14:40 research this initiative reflected their 14:42 shared commitment to making a lasting 14:45 impact on the 14:46 world in 2016 Chan and Zuckerberg set an 14:51 ambitious goal to cure manage or prevent 14:54 all diseases within Max's lifetime they 14:58 pledged three billion dollars toward 15:00 this cause through their 15:02 initiative education remained a 15:04 Cornerstone of their 15:06 philanthropy Chan and Zuckerberg opened 15:09 the primary school in 15:11 2015 a nonprofit institution serving 15:14 students from lowincome 15:17 families they also donated $75 million 15:21 to a San Francisco Public hospital it 15:24 was in 2015 that Facebook founder Mark 15:27 Zuckerberg and his wife Priscilla 15:29 donated $75 million to help build San 15:32 Francisco General state-of-the-art 15:34 Trauma Center which was renamed in 15:37 Zuckerberg's honor though the name later 15:40 became a point of controversy due to 15:42 allegations against 15:44 Facebook their family grew again in 15:46 August 2017 with the birth of their 15:49 second daughter August Zuckerberg took 2 15:53 months of paternity leave emphasizing 15:56 the importance of family time the 15:59 couple's lifestyle though undoubtedly 16:01 influenced by their wealth remained 16:03 grounded in meaningful 16:05 Traditions they traveled extensively 16:08 dedicating two weeks each year to 16:10 overseas 16:11 vacations these trips which included 16:14 visits to Dubai Mumbai and China often 16:18 revolved around reconnecting with Chan's 16:21 Heritage Zuckerberg even learned 16:23 Mandarin to strengthen his ties to her 16:26 culture in 2018 their real estate 16:29 portfolio expanded further with the 16:31 purchase of two Waterfront Estates in 16:33 Lake Tahoe for $59 16:36 million the properties boasting 600 ft 16:40 of private Waterfront access exemplified 16:43 their preference for Serene 16:45 Retreats amid their busy lives the 16:48 Zuckerberg's occasionally offered 16:50 glimpses into their home life such as 16:52 making Chala bread with their daughters 16:54 in 16:56 2019 the couple's dedication to 16:58 addressing Global challenges continued 17:00 during the covid-19 pandemic through the 17:04 Chan Zuckerberg initiative they formed a 17:06 task force to enhance testing 17:08 capabilities in the Bay Area 17:10 demonstrating their commitment to Public 17:13 Health in September 2022 the 17:17 Zuckerberg's announced they were 17:18 expecting their third child their 17:21 daughter Aurelia Chan Zuckerberg was 17:24 born in March 2023 completing their 17:27 family yet even as his personal life 17:31 expanded Mark Zuckerberg's professional 17:34 Focus remained firmly fixed on an 17:36 entirely different kind of world one not 17:39 bound by physical 17:42 limitations Mark Zuckerberg's 17:44 metaverse for a while Mark Zuckerberg's 17:47 metaverse dominated Tech news the 17:51 promise of an immersive virtual reality 17:53 world that was so engaging so 17:56 captivating that people would willingly 17:58 spend a por of their lives within it 18:00 seemed almost like a futuristic dream 18:02 come true at first the idea was exciting 18:06 filled with 18:08 potential the metaverse was to be a 18:10 space where users could interact explore 18:13 and exist in a digital universe that 18:16 went beyond the limits of the physical 18:18 world yet despite the initial hype the 18:22 metaverse reality was far from what 18:25 Zuckerberg and meta had 18:26 promised what was once seen as the 18:29 future of the internet quickly descended 18:31 into a cautionary tale of lofty 18:34 Ambitions and unmet 18:37 expectations when Zuckerberg unveiled 18:39 his metaverse he dismissed critics who 18:42 questioned the Project's 18:43 viability many pointed out that tech 18:46 companies were leaning too heavily into 18:48 the digital world potentially at the 18:51 expense of real world 18:53 connections however Zuckerberg's 18:55 dismissiveness of this criticism soon 18:58 appeared to be 18:59 misguided as the project progressed it 19:02 became clear that the promises made 19:04 about the metaverse a seamless immersive 19:08 experience that would revolutionize how 19:10 we interact with the internet were 19:14 overblown at its Inception the metaverse 19:17 was seen as a transformative idea in 19:20 fact Zuckerberg went so far as to 19:22 Rebrand Facebook entirely changing its 19:25 name to meta in October 2021 one to 19:29 reflect this new Focus tonight the 19:31 company formerly known as Facebook is 19:34 now called meta that's the new corporate 19:37 name for the parent company overseeing 19:39 Facebook Instagram and messenger he 19:42 described the metaverse as the future of 19:44 the internet where users could engage in 19:47 activities as if they were truly present 19:50 with one another the marketing was Grand 19:53 users could make eye contact feel like 19:56 you're right in the room together and 19:59 immerse themselves in this new digital 20:02 Dimension Zuckerberg's Vision seemed to 20:05 promise a world where we could do almost 20:07 anything we could imagine and perhaps 20:10 that was its first major 20:12 flaw Zuckerberg's words backed by a 20:15 slick promotional video presented a 20:18 Utopia of connectedness and 20:21 interaction media Outlets like The Verge 20:24 published long glowing profiles of the 20:27 metaverse lauding its potential to 20:29 change the world the tech industry 20:32 seemed eager to buy into the narrative 20:35 casting Zuckerberg's metaverse as the 20:37 next big thing but the reality did not 20:40 match the dream the virtual worlds 20:43 portrayed in the promotional material 20:45 were sleek and futuristic but what users 20:49 actually experienced was often 20:52 disappointing the metaverses flagship 20:54 product Horizon worlds became emblematic 20:58 of its many 20:59 shortcomings to interact in this space 21:02 users needed to wear an Oculus headset a 21:05 bulky cumbersome device that added to 21:08 the barrier of 21:09 Entry while Horizon worlds was presented 21:12 as the gateway to this Brave New World 21:15 the actual experience was a far cry from 21:18 what Zuckerberg had promised the 21:21 graphics were often criticized as 21:23 outdated akin to early 2000's video game 21:27 technology leaving many to question the 21:30 viability of VR as a mainstream 21:33 technology Horizon worlds rather than 21:36 offering a seamless fully realized 21:39 metaverse felt more like a beta product 21:42 that had not yet matured the once 21:45 hopeful 300,000 monthly users a number 21:49 that sounded promising when first 21:51 announced p in comparison to maa's 21:54 billions of users on Facebook and 21:56 Instagram 21:58 Horizon World struggled to maintain user 22:01 engagement with many complaining about 22:04 empty virtual spaces and a lack of 22:07 meaningful 22:08 interaction users would often find 22:11 themselves wandering desolate digital 22:13 environments with the few participants 22:16 who were around often being children 22:19 rather than a broad cross-section of the 22:21 adult population that Zuckerberg 22:24 envisioned a significant challenge the 22:26 metaverse faced was was its identity 22:29 crisis Zuckerberg's Grand Vision was 22:33 ambitious but it was also vague what 22:36 exactly was the metaverse supposed to be 22:40 despite Zuckerberg's attempts to reframe 22:42 the metaverse as a new and improved 22:45 internet there was no clear use case or 22:48 target audience that could drive its 22:50 success the concept of virtual worlds 22:53 where people interact with avatars 22:56 wasn't 22:57 new m massively multiplayer online 23:00 role-playing games like Ultima Online 23:03 and EverQuest had explored similar ideas 23:06 decades earlier with varying degrees of 23:09 success while the metaverse promised to 23:12 build on these ideas it was unclear how 23:15 this new iteration would differentiate 23:17 itself or offer tangible benefits to 23:21 users without a Clear Vision the 23:23 metaverse lacked the foundation needed 23:25 to thrive as a business 23:28 Berg's vague assertions that it would 23:30 span many companies and become the 23:33 successor to the mobile internet left 23:35 investors and analysts scratching their 23:38 heads meta's attempt to push the 23:41 metaverse forward felt more like a 23:43 marketing strategy than a genuine 23:45 product offering despite grandiose 23:48 statements there was little in the way 23:50 of tangible actionable steps toward 23:54 realizing that 23:55 Vision despite the lack of clarity 23:58 businesses across various Industries 24:01 including Walmart and Disney rushed to 24:04 get involved in the 24:05 metaverse companies that had no prior 24:08 connection to the tech World joined the 24:10 metaverse hype train leading to inflated 24:13 projections of its 24:15 potential Wall Street investors eager to 24:18 capitalize on the next big thing began 24:21 to speculate wildly on the metaverse 24:24 future 24:25 success however as meta sunk billions 24:28 into reality Labs its virtual reality 24:31 arm it became increasingly evident that 24:34 the metaverse was not living up to 24:38 expectations the metaverse touted as a 24:41 revolutionary leap for the internet 24:43 started to show signs of strain meta's 24:46 losses in its reality Labs division 24:49 became 24:50 eye-watering by 20203 meta had lost $21 24:54 billion in its pursuit of the metaverse 24:57 despite Zuckerberg's insistence that the 25:00 company was in it for the long haul 25:02 there was little to show for the vast 25:04 sums of money being 25:06 invested As Time passed the tech 25:09 industry's Focus shifted elsewhere a new 25:12 more promising Trend emerged generative 25:16 AI companies like open AI began to 25:19 capture the imagination of investors 25:22 shifting attention away from the 25:24 metaverse and toward more immediate 25:26 practical applications of a I 25:29 technology for meta the fate of the 25:32 metaverse was 25:34 sealed what had once seemed like the 25:36 future of the internet now appeared to 25:38 be a costly 25:40 distraction Zuckerberg's repeated 25:42 assurances that the metaverse was still 25:45 the company's future fell on deaf 25:48 ears in 2023 meta shifted its strategy 25:53 again introducing new features like 25:55 mobile and desktop compatibility for 25:58 Horizon worlds while continuing to 26:01 promote its Oculus headsets and new 26:05 iterations like the quest 26:07 3 despite this there was little optimism 26:11 in the broader Tech Community that the 26:13 metaverse could ever achieve the level 26:15 of mainstream success that Zuckerberg 26:18 had 26:19 envisioned the reality of the metaverse 26:22 was that it was not an immersive Utopia 26:25 it was clunky isolated and failed to 26:28 deliver on its initial 26:30 promises the once glittering vision of a 26:33 virtual world where we would live work 26:36 and play became a cautionary tale for 26:39 the tech 26:40 industry while the whole metaverse 26:42 situation has been a roller coaster for 26:44 Zuckerberg we can't help but wonder if 26:46 his beloved wife would be by his side 26:49 all through this or if their 26:51 relationship will also Fall by the 26:53 wayside like the likes of Bill Gates and 26:56 Jeff Bezos 26:58 while there's no definitive answer to 27:00 this yet only time will tell what's next 27:03 for the babyfaced tech Tycoon and his 27:07 human 27:08 wife what do you think of Zuckerberg's 27:10 metaverse do you think he'll make a huge 27:13 comeback or do you think 27:15 otherwise let us know your thoughts in 27:18 the comments down below we'd love to 27:20 hear from you remember to like share and 27:23 subscribe for more also click the 27:26 following video shown on your screen you 27:29 will enjoy it The Prime Expedition 65.3K subscribers Videos About 191 Comments rongmaw lin Add a comment... @tessietesoro7407 6 days ago Money won't give you the inner peace as a family ; evil will always test you both . 116 Reply 1 reply @alinabrown7924 5 days ago Maybe after she has a third child, she feels depressed and that’s why things didn’t working well. But please! For all your children you guys should have to stay together forever. 54 Reply @thamnguyen6370 5 days ago His wife doesn't need to be as beautiful as a model but a good pediatrician, they are the most perfect couple of Two souls in harmony 90 Reply 5 replies @shiehfanhsu6032 6 days ago What a beautiful purpose driven couple, please stay together for human’s sake! 77 Reply 1 reply @keithlao7853 1 day ago Mark, keeping your original wife is the best choice for your loved family. 6 Reply @buckyvilla5233 3 days ago Stepparents treat their stepchildren WORSE than their biological children, so PLEASE., DON’T DIVORCE 15 Reply @araceliflores9547 5 days ago (edited) PLEASE don’t divorce , work in your relationship. Go to marriage counseling. 92 Reply 5 replies @dabbiana1999 5 days ago Mark is a Taurus sign. We show our love with a lavish gift! 12 Reply @Allen46u5k 1 hour ago YUK YUK 1 Reply @susandy8977 4 days ago Some couples divorce for financial reasons...asset preservation. 12 Reply @rbfarrell1 58 minutes ago Trading in on a newer model is much better than keeping the old one that goes down in value every year. Even if you can afford to have the old one rebuilt,it is still the old one. Reply @carolburnett8372 4 days ago Love and forgiveness is the key, keep on keeping on.........children grow up and need a good environment so that they can bring each of you much, much joy instead of tragedy.....please find pleasure in what God has already given you..........please. 15 Reply @americanknow8232 7 hours ago She financed Facebokk during start up. 1 Reply @ellipticcryptography3490 18 hours ago Human Skills and Capabilities will always prevail over digital fabrications✔️‼️ 2 Reply @miramirez7168 6 days ago (edited) Mark and wife, do not divorce, instead, talk and patch.up ok? Just forgive one another. For your loving wife, childrens, and soon grand children. Please, celebrate new year together. This s the best. Ok? 78 Reply 1 reply @trommelbiel 10 hours ago 21 years is a blessing.God bless them both. May the have the courage to navigate this new terrain. 1 Reply @paulackah5591 2 days ago Oh, God Almighty, PLEASE , SAVE This marriage: ❤❤❤😂😂😂.... 6 Reply @meherunnessa5131 6 days ago Ohh no don’t divorce please 😢 23 Reply @lovemagicandroad 1 day ago Bigger gifts doesn’t mean more love. That’s for sure. 3 Reply @drannoc100 3 days ago The suffering will be the child(ren)… No matter what, the greatness of a mother and a father include their sacrifices for the family. Without that, they aren’t so great. 5 Reply @valeriezaitzieff1462 2 hours ago I think the majority of people prefer to live in the REAL world rather than in a virtual, imaginary world. Reply @maryloudancel7914 5 days ago Chinese wives are loyal. The couple appear bonded. Maybe it is time for the husband to grow out of fantasy, mature and appeal to things of more value when creating and using his God given talents. Walk the path of God rather than creating self into a god. Prayers are needed to help this couple stay together and for husband to see reality away from virtualities. That would be the essence of helping humanity. Step outside of communication and growing money. Appeal to the people’s soul. 21 Reply 1 reply @donporter8432 5 days ago Virtual Reality is too unreal. 4 Reply @ninaalmark7584 5 days ago For me the whole Metaverse idea was bound to be DOA 8 Reply @orawancarlile6192 5 days ago This person who made the clip will get his karma kicking back because they are still happily married with 3 kids. 36 Reply 1 reply @toffguy3297 11 hours ago DO NOT DIVORCE... You guys already rich. forgive each other. Nobody is perfect. 4 Reply @priciliamc1176 8 hours ago You couple’s marriage has been a family role model. It's better to stay together than to get divorced. Your kids need you and they are innocent. Reply @mayphan2872 5 days ago I don't believe this is true???? 15 Reply @garnetrose6162 5 days ago It really isn’t greener on the other side. Water and nourishment keeps it green 5 Reply @Sayitlikeitis-jn6xe 5 hours ago What’s the HUGE announcement?? Reply @NakedEyes-lm3bj 5 days ago No way! Not true 9 Reply @dauanbryant307 3 days ago 21 years is a long time, unhappy is a long time. 2 Reply @trommelbiel 9 hours ago Pray for their marriage to succeed. May God continue to guide them. I don't expect Mike to be fiddling with virtual reality side of this with a wife and children. He should know they are real. Reply @medeleneilagan 1 hour ago Please don't divorce Reply @ahvc6180 2 hours ago Mark, take your loses and keep your family. Reply @mariademartini157 1 day ago It’s not about beauty is about heart, he loves power. Reply @ofeliadavenport8006 10 hours ago Oh no! Don't divorce please.This is not happening Reply @christianhansen3292 3 days ago why a shot flashing DVD when most folks dont buy them no more? even redbox failed recently.. 1 Reply @esthershelley394 5 days ago It's 'Happy Wife, Happy Life' 4 Reply @jenniehu2129 5 days ago Why rich n favor people have to be divorced???? They need to be attended church believe in Jesus read His Words for their solutions that is only way their whole family need Jesus Christ ✝️👍✝️👍 8 Reply 1 reply @AlisiaT-n5d 13 hours ago (edited) she mad because her husband voted for Kamala Reply @kathieli4844 10 hours ago It’s ver hard to keep remaining both family and business at same time in the good terms. Reply @Jeanniekret 5 days ago It seems the saying “be careful what you wish for” seems appropriate for so many high profile people! Look at Whitney Houston, Elvis, Charlie Sheen, and many more! Why is that? You would think that they have reached the pinnacle of their dreams and then somehow fall apart! 2 Reply @kilchasekyra9336 6 hours ago First marriage is always best, please stay married, there are no one good out there for your both. Reply @Belikeabutterfly-v7z 4 days ago Any relationship i saw it survived ... one was kind and one had the capacity of receiving that kindness Reply @ellipticcryptography3490 18 hours ago Not much backbone for Trust in Meta‼️ 1 Reply @ldu1905 4 days ago The video title cheated everyone! So low! 🤮🤮🤮 11 Reply 1 reply @michaellopez7051 5 days ago This online exploration is for me, quite dangerous, all abstract! Please, let’s go back to simple relatable things!!! 16 Reply @conniecooley1307 5 days ago That's just policibility... to bring them attention much needed at this time. 3 Reply @dianeproffitt837 6 days ago Bet she’d much rather have a PRESENT partner/HUSBAND & daddy rather than an over the top extravagance/tackiness. 9 Reply @ageeibc6029 1 day ago It's very sad for the kids. They are victims. Reply @VivianStripling 6 days ago He'll do fine Mr. Zuckerberg will. He always gets us ahead. He'll come out ahead. 🌏🥰 11 Reply @rubychu9498 1 day ago Marry a workaholic man is much better than an alcoholic or a womanizer. Pricilla has power and wisdom to save her marriage. Make life more fun and meaningful for him to leave his working desk is the first step to do. It's a shame to see a such fine man leave. It's a pity to see her trapped with three daughters alone. Reply @ChengCha-x5x 6 days ago They made a good couple, Zack is not a good looking ma, and his wife isn't good looking. They both look regular, but rich.... 74 Reply 10 replies @masseysubra3165 2 days ago Success in America is a doctored event. If you have an idea and if the know corporations like Vanguard, state street, Black rock and other billionaires you can be success. But Americans often consider money as success, success is not money but how one conducts his life transgenerational. These are the Hall mark of Successful people; Hope, Faith, Believe, Love, Peace Patience, unity, Honesty, Harmony, Integrity, Responsibility, Loyalty, Sincerity, Trust, Goodwill, Charity, Friendship etc. etc. These are some of the qualities that build life. When money is the only motive life will fail, America is the fastest failing nation. America must go to older culture and learn what is all about life. Reply @mountcarmel7116 4 days ago Great wife never comments about her husband unless she genuinely believes it revitalizes him. Reply @rozyue1177 6 days ago Is this for REAL ? 10 Reply @emmarod9081 3 days ago Focus is no longer on the Creator of the Universe. It’s not bad though, but “attentiveness” to the Creator diminishes. 1 Reply @dekdeknav9366 4 days ago You fooled us by talking about their divorce that you didn't really say much. 2 Reply @jpoupart23 5 days ago Another new quick woman millionaire?… 5 Reply @vince73 5 days ago Lovely wife ? 😂😂😂 Well, he is not terrific either ! 5 Reply @lovemagicandroad 1 day ago I think he’s crazy about her. I think she’s the dominant in their relationship. Reply @maryclairecruzado4288 2 days ago this means the man is lonely 1 Reply @bicolanasilyzel4529 20 hours ago when God is the center of the family, the result is definitely good. but when there is money and too much ambition in the middle of the family, they will definitely not be happy. human commitment is uncertain but if the Lord GOD promises it will surely come true Reply @daxoder 5 days ago Green statues portray a green alien on a chrome silver spaceship.👽 1 Reply @paminology6622 2 days ago then Mark will date models, true love not exist.. 1 Reply @brucevannguyen 1 day ago I'm poor and my relationship is just as bad because not making enough money. I think not too much money and not to little money is best for relationships. Today time relationship is all about money. I don't care what anyone say. It's all about money. Just too much or too little will be a problem. Reply @deebhai6540 3 days ago (edited) So heavy alimony is coming 😂 1 Reply @lovemagicandroad 1 day ago The statue is creepy. Reply @miramirez7168 6 days ago (edited) Do not follow your friend, be the best couple ok? Merry christmas and happy new year. 8 Reply @janineshideler4051 1 day ago If this Metaverse makes it there will be many divorces across the world... Reply @lawrencekim8664 1 day ago I ALWAYS SUSPECTED ZAC IS A WEIRDO ! 1 Reply @brendayoung6738 3 days ago There is a reason why a woman would walk away from a billionaire no time for family just time for making money and more money and more money .very lonely woman. Reply @janetjones5405 2 days ago I wish Meta verse was educational 1 Reply @neliaortiz3367 5 days ago No we r human being 2 Reply @susanrenken7317 4 days ago He looks at her with love and longing.. 1 Reply @mil546 2 days ago I hope that they won't divorce, sad. Reply @brianhong301 1 day ago HUMAN RELATIONSHIP IS SUPER COMPLEX AFFECTED BY MULTITUDE OF FACTORS !@#$ SUCH JUST THE WAY IT IS !@#$ NO FAULTS OF EITHER PARTY !@#$ ITS ALWAYS THE CHILDREN AFFECTED THE MOST !@#$ Reply @ArielaShines 11 hours ago (edited) Second Life is the still thriving and existing 21 year reaization of a Metaverse. Zuck should get with Rosedale and hash out some ideas instead of insisting he has the better product. Reply @MAY051260 2 days ago They're still together. Why do you wish ill on mark z n pricilla? You dont have a happy family do you? 1 Reply @principalyinglicollege5087 1 day ago He will come back successfully Reply @ninaalmark7584 5 days ago Were they nuts, going to MacDonalds in Italy!!! 1 Reply @WTHH2 5 days ago Took care of everbody's business except his own home business.!?!?!? 8 Reply @nadiaalbert4336 6 days ago That pre nuptial but be iron Clad 🤷🏻‍♀️ 8 Reply @MyHungho 4 days ago Let me think, Spit the income , in case mark getting in jail , only 1/2 lost 1 Reply @SeanPan-it3jm 2 days ago When China kept Facebook out....... Reply @TinaMBaker 2 days ago I don’t believe that they going to get divorced. It might be a IRS purposes to get away to pay a lot. Reply @stevesung2150 1 day ago They are the best matching couple each other. Divorcing doesn’t get you to any better place but REGRET!!!…. Reply @caridology104 4 days ago Asset allocation. 1 Reply @handsomewong2469 1 day ago Divorce him and take the money Reply @Omicronthewiperofyouknow... 19 hours ago She seems to have gain some pounds since they got togheter. That's... Well... He looks much younger than her. Reply @donaldmarshall4159 18 hours ago Liar ...because he s a heavy pocket How can she leave him Reply @SamuelGluck-g3d 1 day ago You both could definitely come together to produce the best Halloween horror movie ever with the Lowest production cost ever since both of you do not require any make-up or fadeaway for your faces. You both are made for it and it'll be the scariest movie ever. Should you listen to my advice, please don't forget my royalties for the recommendation Reply @supernaturaltv-vq3ev 4 days ago Their evil plans will be deleted, Lord is about to Come Back and all the evil doers will be erased from earth as their malevolous plans and laws. Reply @Gerald-r1l 5 days ago Come on! Mark tends to lose his sense of reality. Facebook started as a good idea, but his obsession for making money changed his 'algorithm' to become a menace to young people. Look at these wealthy tech people. Too often their wealth corrupts their sense of empathy and respect for the people who use their services and products. Prime example: Elon Musk. Enough said! 3 Reply @Kopyori13Montano 1 day ago Think your kids do you think life is funny things New generation will become worst of being irresponsible parents.. Reply @sharonolson5782 4 days ago Great, now let him get the hell out of Hawaii! Reply @bo13416 4 days ago She has got a beautiful smile Reply @qake2021 2 days ago 😁😁😁 INTJ is NOT very romantic and devoted. ✌️😁✌️😁✌️ Reply @PassPortExtraordinaire 5 days ago What do you want? Reply @trucmaitran3469 1 day ago This YouTuber try to pull us. He gets more money from YouTube when he gets more views. Reply @neringaetasugomaite8794 17 hours ago BAD IDEA Reply @SeanPan-it3jm 2 days ago TikTok raked the marriage????? Reply @choifayue9848 4 days ago what kind of vid is this? you have no content of annoucement on their marriage as you stated, nada, as far as his metaverse is concerned, it is doom to fail, and will be a burden for him for a long long time PEACE Reply @UKindness4 6 days ago These guys are creepy. Who would wNt any of them unless they were gold diggers. 9 Reply 2 replies @somc4542 4 days ago Ask ourselves whether we want to live in the real world or the metaverse. Reflect deeply on whether Facebook and Instagram truly benefit us mentally and enhance our lives beautifully. Another person I’d like to point out is Elon Musk. Again, we should ask ourselves whether we want to focus on preserving our beautiful Earth or envisioning a future on Mars. Both individuals have something in common. Reply @soanguyen4672 1 day ago Haha, you believe that? Reply @stevev4863 4 days ago He should fire his hairdresser Reply @annemosbergen3951 20 hours ago META Reply @maily8388 2 days ago I don’t believe this story one bit, it’s simply a click baits more or less. Reply @game-mifi 2 days ago shes preping Reply @amandadrew6861 3 days ago No please Reply @Smiley_101 5 days ago I get his vision but I don’t think it going to grab as many audiences or cross over other than it about 1-2 decades behind . However I think there a cool way to get this to be very popular 😂 but I’m no idiot giving my ideas away .. Reply @ratnasurin 1 day ago 28 Dec ‘ 2024. / Reply @ermarios4281 5 days ago Hope there is no third party or domestic violence. Reply 1 reply @supernaturaltv-vq3ev 4 days ago 6 0 9 😱👹👺💀☠ Birds of a feather... Reply @JamesNglee 3 days ago Told you stay away from Mar-a-Lago. 😂 Reply @lawrenceonuorah6019 3 days ago No one owns the earth. The hand has 5 fingers. Which one do you point to your brothers and sisters?. In all 4 is pointing on you. Every stone that goes up must come down. From the current prophet of Umuezumezu Reply @Mushsayer 5 days ago Let her take the half of his money. Reply @charlielua 5 days ago Dont divorce for the sale of children unless their is 3rd. Party Reply @roseoreillysievers6057 3 days ago Your story is too long, cut to the chase Reply @wat8432 5 days ago What a Disaster!!!!🤬 Reply @albundy363 3 days ago Commie’s assess?? Reply @sannuam6383 5 days ago Wow!! It’s Crazy the Asian women wants to divorce the husband it’s really? 1 Reply @emmasolis4130 5 days ago ❤❤ HAVE MERCY HAVE GRACE It seems meta is more on fantasy dream far from reality . IT'S fantasy driven 😮 ❓ 1 Reply @rinacilliers9835 4 days ago Just don't blame me for hid divorce ok Reply @roseaguilar1295 4 days ago Nooo do not separate no not divorce sir @Meta Reply @sunshine201063 5 days ago OMG....!.the slow tedious monologue. Reply @JoyceLynn-q3m 2 days ago A bit too much . Reply @maraniatakapua3953 4 days ago Divorce Reply @jennifersabanal5183 1 day ago Cluckbait Reply @alicjakijewski3 5 days ago PROSTE ZAMAWIANIE KLIJENTELI BEZ PRZERWY NARKOTYKU WYMAGA KONTROLI DLATEGOBYL ZAJETY Reply @ajasisouk1997 3 days ago I got a # of Facebook I have problems with deleted my Facebook Reply @alicjakijewski3 5 days ago DOWUD BOSS MIESZKANIA NIE PALI TYLKO BZYKMPNIE RANO MAASZ REUMATYZM I KAZE CI TERMOMETR POKAZAC, NIEMOZESZ OPTRAC NIC BO ZIMNO I CZYM GDOZI WILGOCIA MOKREPRANIE ALE BIZNES NA LUDZIACH TAK SIE ROBI CI CO IDA DO PRACY NIE POTRZEBUJA ciEPLA ITAKI POSTEP BIZNESOWY IDZIE? Reply @richardlangcay9903 5 days ago Fraud Reply @jaimeesparza8426 15 hours ago text-green-game-overperson-turqouise-waving Reply @tcabocastoriess4 4 days ago bruhhh clickbate Reply @SuzanaMantovaniCerqueira 5 days ago 😮 Reply @annemosbergen3951 20 hours ago I Reply @almapinon4299 5 days ago Please go to Marriage Counselling,don’t follow Jeff Bizos and ELON MUSK ur friends,be the best Couple and Merry Christmas,Happy New Year! divorce is not the answer… 2 Reply @carinatan5202 1 day ago There is no forever if christ is not in your list all people who are rich they dont care if they separated because of there wealth Reply These are the Hall mark of Successful people; Hope, Faith, Believe, Love, Peace Patience, unity, Honesty, Harmony, Integrity, Responsibility, Loyalty, Sincerity, Trust, Goodwill, Charity, Friendship etc. etc. These are some of the qualities that build life. When money is the only motive life will fail, America is the fastest failing nation. America must go to older culture and learn what is all about life.